全文获取类型
收费全文 | 551篇 |
免费 | 115篇 |
国内免费 | 9篇 |
专业分类
林业 | 429篇 |
农学 | 4篇 |
基础科学 | 7篇 |
16篇 | |
综合类 | 94篇 |
农作物 | 7篇 |
水产渔业 | 7篇 |
畜牧兽医 | 34篇 |
园艺 | 6篇 |
植物保护 | 71篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 30篇 |
2014年 | 81篇 |
2013年 | 18篇 |
2012年 | 28篇 |
2011年 | 19篇 |
2010年 | 44篇 |
2009年 | 59篇 |
2008年 | 48篇 |
2007年 | 44篇 |
2006年 | 63篇 |
2005年 | 104篇 |
2004年 | 57篇 |
2003年 | 29篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1988年 | 2篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
排序方式: 共有675条查询结果,搜索用时 15 毫秒
1.
论电子阅览室的知识导航与信息服务 总被引:3,自引:0,他引:3
尤如春 《农业图书情报学刊》2004,16(11):83-85
本文立足于知识导航与信息服务,对网络环境下电子阅览室的服务内容、服务保障、目前面临的现状进行了论述,并提出了服务对策。 相似文献
2.
In order to develop a rapid, sensitive and specific qPCR assay for detection and quantification of Tomato yellow leaf curl virus (TYLCV), a pair of primers and TaqMan probe were designed according to the conserved sequence of known TYLCV isolates. Combining with MNP technique, a novel MNP-qPCR detection method was established and verified based on specificity, sensitivity and reproducibility tests. The results indicated that the Ct value of plotted standard curve showed good linear relationship(R2 =0.9994)with the log of copy number of template. The established method showed a high specificity for TYLCV detection without crossing reaction with Tomato severe leaf curl virus and Tomato yellow leaf curl Sadinia virus, and was 10-fold more sensitive than routine PCR. Both coefficients of variation were less than 2%, indicating a good reproducibility. We have provided a novel method for detection of TYLCV in plant samples rapidly and quantitatively. 相似文献
3.
从河南省10个地市采集的小麦病根样品中分离得到82株病原分离株, 通过形态特征观察、回接致病性测定、以及分子生物学的方法对所分离菌株进行鉴定。其中47个菌株菌丝分支处都形成典型的“∧”状;子囊壳埋生或半埋生, 子囊棍棒状, 子囊孢子线形, 稍弯曲, 无色, 具有5~10个分隔, 大小为68~94 μm×2~4 μm;对82个分离株rDNA-ITS区进行PCR扩增和序列测定, BLAST序列比对结果表明, 47株菌株与小麦全蚀病菌的ITS序列同源性达97%~99%, 据此确定47株菌株为小麦全蚀病菌。应用小麦全蚀病菌4个变种的特异性引物进行PCR扩增都得到禾顶囊壳小麦变种(870 bp)的特异性片段, 鉴定47株菌株均为禾顶囊壳小麦变种 (Gaeumannomyces graminis var.tritici)。 相似文献
4.
5.
本研究选用39只140~150g SPF级SD大鼠作为动物内毒素血症模型,运用组织学常规HE染色和PAS染色技术,采取定性、定位和定量相结合的方法,通过观察不同试验组大鼠肠黏膜杯状细胞(GC)的数量和分布的动态变化来探讨内毒素(ET)对肠道黏膜免疫系统的影响。结果表明,ET能够破坏肠道黏膜的正常结构,影响肠黏膜的正常功能,同时能够在不同程度上降低GC数量,从而破坏肠道黏膜免疫系统。 相似文献
6.
{{each article.authors author i}} {{if author.authorNameCn && author.authorNameCn != ''}}{{@ author.authorNameCn}}{{if i != article.authors.length - }} {{/if}} {{/if}} {{/each}} 《中国城市林业》2010,8(5):58-59
"头顶云雾脚踩霜,荞麦粑粑萝卜汤"这曾是旧光明村人生活的真实写照。光明村位于漾濞彝族自治县东部,苍山西坡腹地,居住着彝、汉、白、傣、傈僳5个民族,少数民族人口占总人口的65.04%。
相似文献
7.
苄嘧磺隆与尿素混用对陌上菜吸收除草剂的影响 总被引:4,自引:0,他引:4
14C-苄嘧磺隆与尿素混用,处理后1h,陌上菜[Lindernia procumbens (Krock.) Philcox]植株的14C放射性计数达到高峰.然而,14C-苄嘧磺隆单用,陌上菜植株的14C放射性计数随时间延长而缓慢增加,直至48h仍显著低于1h的除草剂与尿素混和的14C放射性计数.处理后24h,14C-苄嘧磺隆在陌上菜植株中的分配以根系最高,茎次之,叶最低.14C-苄嘧磺隆+尿素浸根,促进陌上菜植株对除草剂的吸收,但涂抹叶片时,无论单用还是混用,陌上菜植株均不吸收除草剂. 相似文献
8.
9.
10.
McCain SL Flatland B Schumacher JP Clarke Iii EO Fry MM 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(4):474-479
Background: Advantages of handheld and small bench‐top biochemical analyzers include requirements for smaller sample volume and practicality for use in the field or in practices, but little has been published on the performance of these instruments compared with standard reference methods in analysis of reptilian blood. Objective: The aim of this study was to compare reptilian blood biochemical values obtained using the Abaxis VetScan Classic bench‐top analyzer and a Heska i‐STAT handheld analyzer with values obtained using a Roche Hitachi 911 chemical analyzer. Methods: Reptiles, including 14 bearded dragons (Pogona vitticeps), 4 blue‐tongued skinks (Tiliqua gigas), 8 Burmese star tortoises (Geochelone platynota), 10 Indian star tortoises (Geochelone elegans), 5 red‐tailed boas (Boa constrictor), and 5 Northern pine snakes (Pituophis melanoleucus melanoleucus), were manually restrained, and a single blood sample was obtained and divided for analysis. Results for concentrations of albumin, bile acids, calcium, glucose, phosphates, potassium, sodium, total protein, and uric acid and activities of aspartate aminotransferase and creatine kinase obtained from the VetScan Classic and Hitachi 911 were compared. Results for concentrations of chloride, glucose, potassium, and sodium obtained from the i‐STAT and Hitachi 911 were compared. Results: Compared with results from the Hitachi 911, those from the VetScan Classic and i‐STAT had variable correlations, and constant or proportional bias was found for many analytes. Bile acid data could not be evaluated because results for 44 of 45 samples fell below the lower linearity limit of the VetScan Classic. Conclusions: Although the 2 portable instruments might provide measurements with clinical utility, there were significant differences compared with the reference analyzer, and development of analyzer‐specific reference intervals is recommended. 相似文献