棕仁粕基础营养分析及其在尼罗罗非鱼养殖中的应用研究

本文对比研究了棕仁粕、豆柏、花生粕的基础营养及体外胃蛋白酶消化性,通过在实验饲料中添加2％、6％、10％的棕仁粕制备了四份等氮等能的饲料,初步评价了添加棕仁粕对罗非鱼生长、体组分以及血清学参数的影响.结果表明:棕仁柏作为蛋白水平相对略低,纤维含量较高的植物蛋白替代源,其体外胃蛋白酶消化率与豆粕和花生粕无明显差异,可以作为膳食饲料组分适量的添加到水产动物饲料中,而不会影响罗非鱼的正常生长;在罗非鱼养殖实验中,添加2％棕仁粕的饲料组,罗非鱼的增重率,饲料系数,蛋白利用率、鱼体的蛋白组分以及血清学参数与对照组相比无显著差异(P＞0.05),添加至6％～10％后,罗飞鱼的生长表现开始下降,饲料系数开始上升,鱼体蛋白组分下降(P＜0.05).作为棕仁粕在水产养殖领域的初步探索性应用研究,研究认为棕仁粕是可以用于水产饲料的成分使用的,添加比例不宜超过6％.     

The Influence of Oral Corticosteroids or Declining Allergen Exposure on Serologic Allergy Test Results

Abstract— Twenty-seven proven atopic dogs had a serologic allergy test performed on the day of skin testing and again after either a 21-day course of corticosteroid treatment or 60 days without therapy during which time exposure to botanical aeroallergens was reduced or absent. There was no statistical difference in preand post-corticosteriod treatment results. There were significantly more positive scores recorded in the 60-day follow-up samples in the untreated dogs. Résumé— Vingt-sept chiens ayant une atopie prouvée ont subi des tests sérologiques d'allergie avant et 21 jours après une corticothérapie ou 60 jours sans traitement et sans exposition pollinique. Il n'y avait pas de difference statistiquement significative avant et après le traitement corticoïde. Il y avait beaucoup plus de résultats positifs à 60 jours chez les animaux ne subissant aucun traitement. Zusammenfassung— Bei 27 Hunden mit nachgewiesener Atopie wurde ein serologischer Allergietest am selben Tab wie der Hauttest und erneut entweder nach 21-tägiger Kortikosteroidbehandlung oder nach 60 Tagen ohne Therapie durchgeführt. Bei der zweiten Gruppe wurde die Exposition gegenüber pflanzlichen Aeroallergenen reduziert oder fehlte. Es gab keinen statistisch signifikanten Unterschied bei den Ergebnissen vor und nach Korikosteroidtherapie. Es trat eine statistch signifikant höhere Zahl positiver Reaktionen in den Proben der nicht behandeltentiere nach 60 Tagen aug. Resumen Veinti-siete perros en los que se había diagnosticado previamente atopía, se hizo un test serológico de alergia simultáneamente con tests de inyecciones intradérmicas durante el mismo día, volviendose a repetir a los 21 días despues del tratamiento con corticosteroides, o después de 60 días sin ningun tipo de medicacion, durante en cual exposición a aero-alergenos botánicos fué minima o inexistente. No se observó diferencia de tipo estadístico en animales antes o despues del tratamiento con corticosteroides. En los animales que no recibieron medicación, después de 60 días se observó un aumento significativo de respuestas positivas.     

厦门市同安区猪伪狂犬病流行病学调查

猪伪狂犬病是《国家中长期动物疫病防治规划（2012-2020年）》中优先防治的16种动物疫病之一,到2020年全国所有种猪场猪伪狂犬病都要达到规划设定的考核标准。厦门市同安区为了配合做好猪场净化工作,掌握全区猪群猪伪狂犬病的感染状况,对其进行流行病学调查,估计辖区内猪群猪伪狂犬病的血清学流行率。本研究使用猪伪狂犬病病毒gE-ELISA试剂盒,共检测991份血清样品,并实地调查20家猪场,了解猪场饲养管理和免疫情况。结果显示,同安区猪伪狂犬病血清学场流行率为65.0%,个体真实流行率为47.5%（44.5%~50.4%）。     

河北省猪弓形虫的血清学检测

应用间接血凝试验(IHA)对采自我省11个地区定点屠宰厂,养殖场及散养户的1300份猪血清进行了弓形虫病抗体检测,结果表明,阳性464份,阳性率为35.69%。     

Effect of drug-containing serum of Fushen Jiangzhuo formula on anti-fibrosis factors HGF and BMP-7 in rat renal interstitial fibroblasts with renal tubulointerstitial lesion

AIM: To study the protective effects and mechanism of Fushen Jiangzhuo formula (FSJZ) on the rat renal interstitial fibroblasts with renal tubulointerstitial lesion. METHODS: The serum containing FSJZ and blank control serum were prepared. The rat model of mesangial proliferative glomerulonephritis (MsPGN) was established and extended the time of modeling to 20th weeks for developing tubulointerstitial lesion naturally. The rat renal interstitial fibroblasts at the end of 12, 16, 20 week of modeling were isolated and cultured. The effects of FSJZ on the expression of hepatocyte growth factor (HGF) and bone morphogenetic protein-7 (BMP-7) at mRNA and protein levels in the pathological renal interstitial fibroblasts were determined. RESULTS: The anti-fibrosis factors HGF and BMP-7 were changed in pathological renal interstitial fibroblasts. Renal tubulointerstitial lesion significantly down-regulated the expression of HGF and BMP-7, while the drug containing serum of FSJZ significantly up-regulated the expression of HGF and BMP-7. CONCLUSION: Drug containing serum of FSJZ has protective effect on pathological renal interstitial fibroblasts by regulating the mRNA and protein expression of HGF and BMP-7.     

Effect of serum containing Fushen Jiangzhuo formula on TGF-β1/Smads signal transduction pathway in renal interstitial fibroblast of renal tubulointerstitial lesion rat model

AIM:To study the protective effects of Fushen Jiangzhuo formula (FSJZ) on renal interstitial fibroblasts in the rats with renal tubulointerstitial lesion. METHODS:The serum containing FSJZ and blank serum were prepared. The rat model of mesangial proliferative glomerulonephritis (MsPGN) was established, and the tubulointerstitial lesion model was developed by extending the time of the MsPGN model to 20 weeks. The renal interstitial fibroblasts from the model rats were cultured at 12, 16 and 20 weeks. The effect of FSJZ on the expression of transforming growth factor beta 1 (TGF-β1), Smad3 and Smad7 at mRNA and protein levels in the pathological renal interstitial fibroblasts was determined. RESULTS:The TGF-β1/Smads signal transduction pathway changed in pathological renal interstitial fibroblasts. The renal tubulointerstitial lesion significantly up-regulated the expression of TGF-β1 and Smad3, and down-regulated the expression of Smad7. The FSJZ-containing serum significantly down-regulated the expression of TGF-β1 and Smad3, and up-regulated the expression of Smad7, thus antagonizing the effects caused by renal tubulointerstitial lesion. CONCLUSION: FSJZ-containing serum has protective effects on renal interstitial fibroblasts. The mechanism may be related to the regulatory effects of FSJZ on TGF-β1/Smads signal transduction pathway.     

A serologic survey of bovine rotavirus infection in Italy

Serum neutralization tests conducted by microtiter procedure on 2970 serum samples from calves, heifers and adult cows from representative regions of Italy indicate that rotaviruses, and the infection which they cause, existed in Italy long before the virus was isolated. At the present time the infection is distributed fairly uniformly throughout the cattle population with both sexes and all age groups being equally susceptible.