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Walter GL McGraw P Tvedten HW 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1992,21(1):23-27
An enzymatic, kinetic method for determining serum lipase activity was evaluated and compared to a standard manual method for use in dogs. The kinetic method was a commercial kit adapted for use on a tandem access clinical chemistry analyzer and utilized a series of coupled enzymatic reactions based on the hydrolysis of 1,2-diglyceride by lipase. The manual method was the Cherry-Crandall technique based on the titration of base against the acid formed by hydrolysis of an olive oil substrate by lipase. The correlation between the two methods was very good (r = 0.94). The reference range for 56 clinically healthy dogs assayed by the kinetic method was 90 to 527 U/L. Diseases associated with a greater than twofold elevation in serum lipase activity as determined by the kinetic method included pancreatitis, gastritis with liver disease, and oliguric renal failure with metabolic acidosis. In some cases, pancreatitis was seen with other clinical problems, such as gastroenteritis, diabetic ketoacidosis, duodenal mass, disseminated intravascular coagulation, and septic peritonitis. Diseases associated with serum lipase activity within the reference range or elevated less than twofold included gastritis, gastric ulcer, cholestasis, phenobarbital-induced hepatopathy, colitis, copper hepatopathy, abdominal hematoma, apocrine gland adenocarcinoma, and thrombocytopenia with pneumonia. 相似文献
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A potentially significant cause of damage to grassland soils is compaction of unsaturated soil and poaching of saturated or nearly saturated soil by animal hooves. Damage is caused when an applied stress is in excess of the bearing strength of the soil and results in a loss of soil structure, macroporosity and air or water conductivity. Severely damaged soils can cause reduced grassland productivity and make grazing management very difficult for the farmer. The actual amount of soil damage that can occur during grazing is dependent on the grass cover which acts as a protecting layer, the soil water content and the characteristics of the grazing animal (weight and hoof size). Assuming that the farmer is knowledgeable about the characteristics of the grazing animal and grass cover, it would be very useful for short‐term operational farm planning to be able to predict when soil water contents were likely to be in a critical range with respect to potential hoof damage. In this study soil moisture deficits (SMDs) which can be derived from meteorological forecasts are evaluated for predicting when soil water conditions are likely to lead to hoof damage. Two contrasting Irish grassland soils were analysed using a Hounsfield servo‐mechanical vertical testing machine to simulate static (285.4 N) and dynamic (571 N) hoof loads on the soil over a range of estimated SMDs (0, 5, 10 and 20 mm). The deficits were analysed with respect to the soil volumetric water content, compression (displacement) and change in dry bulk density. The SMDs imposed in the laboratory were similar to those under field conditions and thus the methods used in this study are applicable elsewhere. The change in dry bulk density following loading (0.2–0.7 g/cm3) was linearly related to SMD (R2 ranged from 0.90 to 0.99), leading to the conclusion that a forecast of SMD can be used to predict when grassland soils are likely to be at risk of damage from grazing. 相似文献
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近年来,国内出现了不少基于城市老街区改造为商业街区的项目。通过对佛山岭南天地项目中有代表性的广场、街道、建筑等节点进行调研,选取开放空间的3个特色要素——空间形态和尺度,传统装饰元素,植物和水景布置,探析"新天地"模式中开放空间营造的特色手法,包括保持街巷形态和宜人尺度,增强街区公共属性;保护传统建筑构件,利用传统材料、技术;保留古树,并结合植物和水景布置,形成多层次的景观系统等。 相似文献
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Group housing is claimed to possibly provide horses with a species-appropriate movement possibility, and hence, better welfare. Thus, this study analyzed the daily walked distances of 51 horses held in one group in a “HIT Active Stable” (Hinrichs Innovation + Technik) in Northern Germany by using global positioning system (GPS) technology during a 7 ½-month time span. The daily walking distances of the whole group, as well as newcomers, were investigated. The horses traveled an average of 8.43 km/day. Linear mixed models were applied. The observation day had a significant effect on the daily walking distances (P < .01) due to season and the available area per horse. The age as covariate also had a significant effect (P < .01). The breed had no significant effect (P = .96). No significant differences were found in sex (P = .69), which can be explained by the fact that only mares and geldings were investigated, which do not show increasing locomotion caused by sexual behavior as stallions do. On six of the first nine days, new horses moved significantly more compared to the remaining 24 of the 30 observation days directly after individuals’ inclusion. This is probably due to more exploration and rank-fighting behavior. Similar walking distances were seen among the horses on the single observation days because all horses had to travel the same distance to reach resources. Further, it is suspected that not all horses can sufficiently live out their urges to move, especially in winter, when pasture is inaccessible. 相似文献
7.
绩效预算是我国部门预算管理改革的重要内容。国家重点实验室专项经费绩效预算安排机制,是财政科研经费绩效预算的典型示例。文章通过剖析国家重点实验室专项经费绩效考评管理机制,对国家重点实验室绩效预算机制推广应用于其他财政专项的范围、考评指标、考评工作方法、考评结果应用等方面提出了意见建议。 相似文献
8.
王玉华 《农业机械化与电气化》2013,(10):91-92
样品管理是实验室工作中的重要环节,规范化、制度化的样品管理工作可保障实验室管理体系的有效运行。阐述实验室样品管理的总体要求与原则,从样品接收、标识、确认、流转、贮存、处理等环节人手,介绍样品全过程跟踪管理方法,为做好实验室样品管理工作提供参考。 相似文献
9.
Kasumi Sudo Mariko Ochiai Naoyuki Aihara Noriyuki Horiuchi Atsushi Yamamoto Sachiko Matsumoto Koji Oishi 《Journal of toxicologic pathology》2021,34(2):137
Batch safety tests (BSTs) of veterinary vaccines are conducted using small laboratory animals to assure the safety of vaccines according to several criteria, including clinical signs and change in body weight. Although the latter is used as an evaluation index in BSTs, there have been no reports on the internal changes that affect body weight during the test period. Therefore, we analyzed BST via pathological examination of the tested animals. Here, BSTs were performed for 176 batches using mice and 126 batches using of guinea pigs. Most of the gross findings could be classified into four lesion types (nodules, adhesions, ascites, no apparent signs), with only one vaccine inducing lesions that could not be classified into any of these four types. Histopathological examination revealed that the reactions caused by BST were pyogenic and/or granulomatous inflammation. Nodular or adhesive lesions comprised more severe pyogenic granulomatous inflammation than ascites or cases with no apparent gross lesions. These nodular or adhesive lesions were more frequently induced by vaccines that contained an adjuvant than by vaccines that did not contain an adjuvant. The cases with “exceptional” gross findings histologically presented severe necrosis of the hematopoietic system. Additional testing showed that these “exceptional” lesions were induced when a specific type of light liquid paraffin was injected along with other vaccine additives. Our results show that body weight loss and/or lesions during BST were induced by proinflammatory properties of the tested vaccines and that BST is a sensitive method for detecting unexpected effects of vaccine components. 相似文献
10.
Test data generated by ~60 accredited member laboratories of the American Association of Veterinary Laboratory Diagnosticians (AAVLD) is of exceptional quality. These data are captured by 1 of 13 laboratory information management systems (LIMSs) developed specifically for veterinary diagnostic laboratories (VDLs). Beginning ~2000, the National Animal Health Laboratory Network (NAHLN) developed an electronic messaging system for LIMS to automatically send standardized data streams for 14 select agents to a national repository. This messaging enables the U.S. Department of Agriculture to track and respond to high-consequence animal disease outbreaks such as highly pathogenic avian influenza. Because of the lack of standardized data collection in the LIMSs used at VDLs, there is, to date, no means of summarizing VDL large data streams for multi-state and national animal health studies or for providing near-real-time tracking for hundreds of other important animal diseases in the United States that are detected routinely by VDLs. Further, VDLs are the only state and federal resources that can provide early detection and identification of endemic and emerging zoonotic diseases. Zoonotic diseases are estimated to be responsible for 2.5 billion cases of human illness and 2.7 million deaths worldwide every year. The economic and health impact of the SARS-CoV-2 pandemic is self-evident. We review here the history and progress of data management in VDLs and discuss ways of seizing unexplored opportunities to advance data leveraging to better serve animal health, public health, and One Health. 相似文献