黄条鰤仔稚幼鱼消化酶活性变化研究

为了解黄条鰤(Seriola aureovittata)早期发育阶段的消化生理特性,测定了黄条鰤胚胎、仔稚幼鱼阶段脂肪酶、淀粉酶、胰蛋白酶和碱性磷酸酶活性变化。结果显示,在黄条鰤仔鱼出膜前胚胎阶段,即能检测到脂肪酶、淀粉酶和碱性磷酸酶活性;初孵仔鱼体内(1 d)初次检测出胰蛋白酶的活性。脂肪酶和碱性磷酸酶比活力在仔鱼孵化后迅速增强(P<0.05),在4 d开口时,2种酶比活力达最高值;淀粉酶比活力在7 d时达最大值;胰蛋白酶比活力在仔鱼阶段缓慢上升,15 d时比活力最大。稚鱼阶段内脏团中脂肪酶、碱性磷酸酶和胰蛋白酶活性基本维持稳定,幼鱼阶段内脏团脂肪酶、碱性磷酸酶和胰蛋白酶活性都呈现上升趋势;稚鱼和幼鱼阶段内脏团中淀粉酶活性下降并基本稳定于较低水平。研究表明,黄条鰤仔稚幼鱼发育过程中,各种消化酶活性变化明显,且与其发育阶段和食性密切相关。在尚未摄食饵料的早期仔鱼体内已存在消化酶,认为其是母源传递而来,不是由外源性饵料所致;幼鱼阶段内脏团脂肪酶、碱性磷酸酶和胰蛋白酶比活力明显提高,这反映出随苗种生长发育,其肠道结构和消化机能逐渐完善,并且对脂肪、蛋白质的需求逐渐增强。     

草原毛虫幼虫的食性及其空间格局

以禾草及嵩草在高寒草甸中的优势度为指标,研究草原毛虫(Gynaephora alpherakii)的食性、空间格局及其与植物群落的相关性。结果表明:草原毛虫的食性具有较强的选择性,其喜食程度排序为莎草科(Cyperaceae)>禾本科(Gramineae)>杂类草,特别对嵩草属(Kobresia Willd.)植物表现出强烈的偏好和倾向性;草原毛虫的分布型受制于其喜食植物的空间格局,在总体上呈聚集分布,但在小尺度下则常呈均匀分布;虫口密度依赖于喜食植物的丰富度和多样性,随着喜食植物的增加而显著上升,随着植物多样性的减少而显著下降;草原毛虫在高寒草甸生态系统中具有平衡物种关系、调控群落结构的功能;采用定向改变草地植物组成的农艺措施实现对草原毛虫数量的人工调控是实施虫害生态防治的有效途径。     

Spawning, early development and first feeding in the gobiid fish Priolepis nocturna

The reproductive behavior, embryonic development and early larvae of Priolepis nocturna are described. Three pairs of P. nocturna began spawning 41 days after acquisition and maintained a 5–10 day spawning cycle lasting beyond several months. Spawning was initiated by the female who signaled her readiness to spawn by displaying to the male. Egg clutch size averaged 1578 ± 51.23 eggs and ranged from 268 to 3121. Egg length averaged 0.82 ± 0.01 mm total length (TL) and ranged from 0.75 to 0.90 mm. Egg width averaged 0.51 ± 0.51 mm total width (TW) and ranged from 0.49 to 0.52 mm. Fertilized eggs were ovoid in shape and attached to the ceiling of provided shelters via adhesive filaments at the proximal end. Hatching rates averaged 97.3 ± 0.51% and ranged from 91.9 to 99.8%. Larvae measuring 1.89 ± 0.04 mm TL hatched 121 ± 0.5 h post fertilization and did not rotate position prior to hatching. Skeletal elements of the chondrocranium were simplistic and dominated by the hyoid, hyomandibulosymplectic cartilage, ethmoid and Meckel's cartilage in first feeding larvae. No elements were added to the cranial architecture by 5 days post hatch (DPH) when larvae measured 2.05 ± 0.04 mm TL. First feeding larvae consumed only dinoflagellates and tintinnids suggesting that feeding was constrained by a poorly developed feeding mechanism. Embryology and larval development are described to 5 DPH.     

Development of wolffish eggs at different temperature regimes

Embryonic development of common wolffish (Anarhichas lupus L.) was studied at constant temperatures 5.0, 7.0, 9.0, 11.0, 13.0 and 15.0°C. Duration of development from egg activation to several morphological stages including 50% hatching was determined. At 5.0–11.0°C, the survival rate of eggs to hatching ranged from 51 to 88% with a tendency to increase at 5.0 and 7.0°C. Morphological anomalies, bacterial contamination and large mortalities were observed in eggs incubated at 13.0 and 15.0°C. The period of hatching lasted from 10 to 50 d in different egg groups. Embryo length and yolk sac volume at identical morphological stages of development showed only slight relation to temperature. At lower temperatures newly hatched larvae were longer and at more advanced stages of ontogeny. Normal numbers of fin rays in larvae (mean values 74 for dorsal fin and 46 for anal fin) were observed at 5.0 and 7.0°C and in most larvae at 9.0°C. At 11.0 and 13.0°C, many rays were absent, with mean values for dorsal fin 60 and 39 respectively and for anal fin 28 and 4 respectively. The approximate upper limit for normal development of fin rays appeared to be 9.0 °C.     

黄海北部大连沿岸虾夷扇贝天然苗采集技术研究

在调查了2005、2006年黄海北部大连海域虾夷扇贝的繁殖期、浮游幼虫的时空分布的基础上,对虾夷扇贝海区天然采苗进行了研究。结果表明,2005、2006年该区域的獐子岛海域、大长山岛海域、广鹿岛海域养殖的虾夷扇贝的繁殖期在4月初至5月上旬,盛期在4月15～25日。4月上旬至6月中旬,在此区域均可发现大量的虾夷扇贝浮游面盘幼虫,不同区域浮游幼虫密度变化很大。5月底至6月初是面盘幼虫集中的附着变态时期,在海区投放附着袋可进行虾夷扇贝天然苗的采集。从各海区虾夷扇贝浮游幼虫的拖网调查和采苗结果看,在虾夷扇贝主要养殖区獐子岛、大长山岛海域和广鹿岛海域,浮游幼虫在浮游的初始阶段能够形成较高的密度,但在附着阶段密度极低,不能采集到大量苗种,但在远离虾夷扇贝养殖区的大李家湾和凌水湾,浮游幼虫的出现比养殖区晚,在幼虫附着期能达到较高密度。能够采集到大量天然苗种。2005年在凌水湾的采苗数量达526±131个/袋(壳长0.6～1 mm),2006年在大李家湾的采苗数量达673±200个/袋(壳长0.6～1mm)。     

Influence of food quality and quantity on the growth and development of Crassostrea gigas larvae: a modeling approach

A biochemically based model was developed to simulate the growth, development and metamorphosis of larvae of the Pacific oyster, Crassostrea gigas. The model is unique in that it (1) defines larvae in terms of their protein, neutral lipid, polar lipid, carbohydrate and ash content; (2) tracks weight separately from length to follow larval condition index and (3) includes genetic variation in growth efficiency and egg quality to better simulate cohort population dynamics. The model includes parameterizations for larval filtration, ingestion and respiration that determine growth rate and processes controlling larval mortality and metamorphosis. Changes in tissue composition occur as the larva grows and in response to the biochemical composition of the food.

The simulations show that genetically determined variations in growth efficiency produce significant changes in larval survival and success at metamorphosis. Larvae with low growth efficiency are successful under a much narrower range of culture conditions than larvae with high growth efficiency. The impact of low growth efficiency is primarily controlled by the ability of larvae to store lipid for metamorphosis. Culture conditions that provide increased dietary lipid counterweigh low growth efficiency. Changes in food quantity and quality had little effect on size at metamorphosis. On the other hand, larval life span and success rate at metamorphosis varied over a wide range depending upon the conditions of the simulation. Food quality and food availability both influence larval life span and, hence, larval survival. As ingestion rate decreases, larval life span increases and cohort survival declines. Increased lipid or decreased protein in the diet improves cohort survival. Changes in carbohydrate content are less influential. If cohort success is significantly affected by mortality during larval life rather than success at metamorphosis, the influence of food quality becomes more complex. The range of food compositions yielding high survival is restricted by a balance between improved success at metamorphosis obtained by increased lipid storage and the shortening of larval life span as a result of more rapid growth, a function of protein availability. These simulations illustrate the strength and utility of numerical models for evaluating and designing hatchery protocols for optimizing yield of C. gigas larvae.     

Rearing performance of Clarias batrachus larvae: Effect of age at stocking on growth and survival during fingerling production

An experiment was conducted to evaluate the rearing performance of different larval stages for optimum growth and survival during fingerling production of Clarias batrachus with an aim to reduce the rearing time of the catfish larvae in a hatchery system. The larvae were reared for 5, 10 and 15 days before stocking them in the nursery tanks. No significant (P < 0.05) difference could be found in length increment at the end of first week of rearing. But the total length was reduced during rest three weeks in five days old larvae compared to that of other two higher age groups. In 5 days old larvae the reduced weight (P < 0.05) was observed during the entire rearing period. However, the specific growth rate (SGR) did not vary among the groups. The survival rate as well as total biomass was decreased while stocking five days old larvae for rearing, compared to that of other two age groups.     

Culture of Eriocheir sinensis Larvae in Ponds in Greenhouse

A total of 15 pools were selected from a greenhouse, and they were randomly and evenly divided into three groups. In each group, one stocking density of Eriocheir sinensis was arranged. The results showed that after 45-d culture, the crab number per kilogram reached about 300 with survival rate of about 20%; the initial stocking density had significantly effect on the body size of E. sinensis on sel , instead on the survival rate of E. sinensis larvae. Therefore, it is feasible to conduct the culture of E. sinensis larvae in a pool in greenhouse.     

狭胸天牛幼虫触角主感器及下颚须和下唇须感受器扫描电镜观察

利用扫描电镜观察了狭胸天牛幼虫触角主感器及其下颚须和下唇须顶端感受器的形态，种 类和分布，并讨论了其结构与功能的关系。结果表明，触角主感器受肾形成唇形，长约９８．０μｍ，宽约２４．５μｍ。下颚须顶端感受器２０个～２５个，下唇须顶端约１５个感受器，根据不同形态，大致分为３种类型；栓锥形，锥柱形和腔锥形。栓锥形和锥柱形感受器分别着生于下颚须和下唇须顶端表面，腔锥形感受器着生于它们的端节侧面。     

梨小食心虫幼虫为害桃梢的空间分布型

为了能准确预测梨小食心虫种群数量、变动规律,对梨小食心虫幼虫在桃梢的空间分布型进行了研究。结果表明,应用经典的理论频次测定法和应用扩散系数等8种指标分析,确定了该虫的空间分布型为聚集分布,其聚集原因Blackith种群聚集均数测定,说明梨小食心虫幼虫在桃梢上的聚集分部主要是由聚集习性所致,同时也受环境条件的影响。