全文获取类型
收费全文 | 2347篇 |
免费 | 66篇 |
国内免费 | 265篇 |
专业分类
林业 | 49篇 |
农学 | 334篇 |
基础科学 | 13篇 |
102篇 | |
综合类 | 815篇 |
农作物 | 540篇 |
水产渔业 | 122篇 |
畜牧兽医 | 430篇 |
园艺 | 253篇 |
植物保护 | 20篇 |
出版年
2024年 | 6篇 |
2023年 | 9篇 |
2022年 | 22篇 |
2021年 | 44篇 |
2020年 | 54篇 |
2019年 | 37篇 |
2018年 | 26篇 |
2017年 | 65篇 |
2016年 | 83篇 |
2015年 | 67篇 |
2014年 | 92篇 |
2013年 | 118篇 |
2012年 | 137篇 |
2011年 | 168篇 |
2010年 | 138篇 |
2009年 | 185篇 |
2008年 | 160篇 |
2007年 | 223篇 |
2006年 | 157篇 |
2005年 | 102篇 |
2004年 | 113篇 |
2003年 | 87篇 |
2002年 | 70篇 |
2001年 | 55篇 |
2000年 | 34篇 |
1999年 | 46篇 |
1998年 | 45篇 |
1997年 | 56篇 |
1996年 | 41篇 |
1995年 | 42篇 |
1994年 | 45篇 |
1993年 | 26篇 |
1992年 | 23篇 |
1991年 | 23篇 |
1990年 | 34篇 |
1989年 | 17篇 |
1988年 | 8篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1963年 | 3篇 |
排序方式: 共有2678条查询结果,搜索用时 15 毫秒
1.
研究了咪唑类化合物KK—42对家蚕卵巢发育以及蛋白质合成的影响,结果表明KK-42对卵巢增重有明显的抑制作用,血淋巴蛋白含量在蛹期变化幅度很小,卵巢蛋白质含量增加也很缓慢;而对照区在化蛹后,血淋巴蛋白急剧增加,第三天达到高峰,以后又迅速下降,与卵巢蛋白质增加相吻合。由此表明,咪唑类化合物KK-42不仅抑制血淋巴蛋质的合成,同时还抑制了血淋巴蛋白向卵巢的转移。 相似文献
2.
AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer. 相似文献
3.
ZHANG Li-rong XIANG Peng XIA Wen-jie CHEN Zhen-guang ZHANG Xiu-ming LI Yan LI Shu-nong 《园艺学报》2002,18(8):896-899
AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK. 相似文献
4.
研究选择21周龄新扬州鸡种公鸡135只,随机分成9组。采用两因子析因设计,分别设粗蛋白质水平为17%、14%和11%,钙水平为3.3%、2.3%和1.3%,构成9种日粮处理。比较在繁殖期全程饲喂不同粗蛋白质和钙水平日粮对种公鸡新城疫水平的影响,结果表明:日粮粗蛋白质水平对免疫后15、30天公鸡体内的抗体水平有显著影响(P<0.05),对免疫90天的水平有极显著影响(P<0.01);日粮钙对免疫后30天的抗体水平有极显著的影响(P<0.01);二者的交互作用对它影响不显著(P>0.05);低蛋白低钙组抗体水平显著高于高蛋白高钙组(P<0.05)。 相似文献
5.
AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC. 相似文献
6.
草菇低温诱导蛋白的初步研究 总被引:4,自引:2,他引:2
低温诱导草菇(Volvariella volvacea)菌丝体一时间后,发现草菇在低温胁迫中产生了新的可溶性蛋白质。应用电泳分离制备技术,分离纯化了草菇菌丝体中的一个低温诱导蛋白。 相似文献
7.
本研究选择20只成龄母雉鸡,采用无氮日粮法和回归分析法比较测定内源代谢氮和维持蛋白质需要量。结果表明,成龄雉鸡内源代谢氮(内源尿氮+代谢粪氮)分别为01888g/kgW0.75·d和02274g/kgW0.75·d;维持蛋白质需要量理论值为267g/kgW0.75·d~3.22g/kgW0.75·d。 相似文献
8.
9.
牦牛瘤胃内饲料蛋白质降解率的研究 总被引:3,自引:0,他引:3
用瘤胃尼龙袋技术测定了11种常见饲料在牦牛瘤胃内的降解率。根据饲养水平,瘤胃食糜的外流速度估计为0.067/h。测定结果表明,玉米、豌豆秸粉、菜籽饼、豌豆、鱼粉、曲拉、肉骨粉、青稞、甲醛鱼粉、甲醛菜籽饼和麸皮的粗蛋白(CP)降解率分别为0.68、0.56、0.44、0.85、0.49、0.55、0.81、0,83、0.38、0,48和0.79,干物质(DM)降解率依次为0.68、0.30、0.49、0.73、0.50、0,54、0,59、0.82、0.32、0.49和0.74。 相似文献
10.
采用滤纸培养皿法,以发芽率、发芽势、根长和苗长为指标,研究细叶旱芹(Cyclospermum leptophyllum(Pers.) Sprague ex Britton et P. Wilson)地上部化感物质对4种草坪草和5种牧草受体植物的种子萌发和幼苗生长的影响。结果表明,细叶旱芹地上部化感物质对4种草坪草和5种牧草的种子萌发和幼苗生长均有抑制作用,其中对种子发芽势抑制最明显,抑制率最高达96.3%;其次是对幼苗根长的抑制作用,抑制率最高达91.1%。通过比较种子萌发和幼苗生长的测定指标,得出5种馏分对草坪草的化感作用为石油醚相>氯仿相>正丁醇相>乙酸乙酯相>水相,而对牧草的化感作用为石油醚相>乙酸乙酯相>氯仿相>正丁醇相>水相。说明不同受体植物对相同萃取相组分的化感作用强弱存在差异,细叶旱芹地上部化感物质主要存在于石油醚馏分中,可进一步从中分离提取活性物质。 相似文献