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蜘蛛毒素对人胃腺癌细胞株BGC-823增殖的抑制作用 总被引:1,自引:0,他引:1
为比较不同蜘蛛毒素对肿瘤细胞的抑制作用,采用四甲基偶氮唑盐比色法(MTT法)测定了不同浓度的虎纹单柄蛛(Ornithocton huwen)、海南单柄蛛(Ornithoctonus hainana)、敬钊缨毛蛛(Chilobrachy jingzhao)和雷氏大疣蛛(Macrothele raveni)4种蜘蛛毒素对人胃腺癌细胞株BGC-823的抑制作用。结果表明:4种蜘蛛毒素对人胃腺癌细胞株BGC-823的细胞增殖均有不同的抑制作用;同一蜘蛛毒素浓度越高抑瘤作用越好,蜘蛛毒素作用时间越长抑瘤效果越强,量效关系和时效关系良好;不同蜘蛛毒素对BGC-823细胞增殖的抑制作用效果不同,以敬钊缨毛蛛毒素作用最强,IC50为0.109μg/μL。 相似文献
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AIM: To investigate the effect of dihydroartemisinin (DHA) adjuvant treatment on enhancing the antitumor effect of 5-fluorouracil (5-FU) against gastric cancer. METHODS: The gastric cancer BGC-823 cells were divided into control group, DHA group, 5-FU group, 5-FU+DHA group and 5-FU+DHA+SIRT1 plasmid group. The viability of BGC-823 cells treated with DHA and 5-FU was measured by MTT assay. The expression of SIRT1 and NADPH oxidase, activation of caspase-9 and caspase-3, and phosphorylation of ASK1 and JNK in the BGC-823 cells treated with DHA and 5-FU were determined by Western blot. The production of ROS and the apoptosis of the BGC-823 cells treated with DHA and 5-FU were analyzed by flow cytometry. RESULTS: Dihydroartemisinin significantly inhibited the expression of SIRT1 and increased NADPH oxidase protein level (P<0.05). DHA increased the sensitivity of BGC-823 cells to 5-FU, thus decreasing the IC50 of 5-FU to the gastric cancer cells. However, transfection with SIRT1 plasmid decreased the cytotoxicity of DHA and 5-FU co-treatment to the BGC-823 cells. DHA promoted the production of ROS and phosphorylation of ASK1 and JNK induced by 5-FU in the BGC-823 cells (P<0.05). However, ROS scavenger N-acetylcysteine (NAC) or JNK specific inhibitor SP600125 inhibited the cell death and activation of caspase-9 and caspase-3 induced by DHA and 5-FU co-treatment (P<0.05). In addition, NAC significantly inhibited the phosphorylation of JNK in the BGC-823 cells co-treated with DHA and 5-FU. However, treatment with SP600125 did not influence the ROS production in the BGC-823 cells, indicating that JNK was the downstream target of ROS pathway. CONCLUSION: Combination of DHA with 5-FU induces caspase-dependent apoptosis in gastric cancer cells through the SIRT1/NADPH oxidase/ROS/JNK signaling pathway. 相似文献
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AIM:To observe the inhibitory effect of interferon-α (IFN-α) on the growth invasiveness and metastasis of human gastric carcinoma cell line BGC-823,and mechanism of its action.METHODS:We detected the influence of IFN-α on the proliferative ability of BGC-823 in cell culture system,the cell vitality with the MTT colorimetric assay,and the cell cycle with flow cytometer (FCM).The regulatory functions of IFN-α to the expression of E-cadherin and matrix metalloproteinase-2 (MMP-2) in tumor cells were estimated by immunohistochemical analysis (S-P).The ultrastructural changes of the junction among the tumor cells were observed under electron microscope.RESULTS:IFN-α can significantly inhibit the growth of human gastric carcinoma cell line BGC-823 in a dose-dependent manner.When the concentration of IFN-α was ≥106 U/L,the cell proliferation can be effectively suppressed,the suppression rate was ≥12.2%,and the blockage appeared at the phase of G1-S of the cell cycle.Under the induction of IFN-α,the expression level of the cell E-cadherin increased while the MMP-2 decreased.The changes on ultrastructure of the cells showed the increased adhesive junctions and the relative compact structure.CONCLUSION:IFN-α can suppress the growth of human gastric carcinoma cell line BGC-823 through its influence on cell cycle.IFN-α can regulate the expression of E-cadherin and MMP-2,make the cell junction closely,so that it has the potential on restricting the invasion and metastasis of gastric carcinoma cells. 相似文献
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YUAN Guangfeng XU Ruiya ZHANG Shubin WANG Li’anLife Science College of Hebei Normal University Shijiazhuang Hebei China Xuanhua Campus of Zhangjiakou Institute of Education Xuangha Hebei China 《食用菌学报》2007,(2)
Different organic solvents (petroleum ether, ethyl acetate and ethanol) were used to extract A. aegerita mycelium and spent culture medium following growth of the fungus in submerged culture. All the extracts exhibited antioxidant and anti-tumor activity that may be linked to triterpenoids present in the extracts. 相似文献
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红香稻823是以自育红香稻恢复系SR33M为母本、自育中间材料F02-80-1为父本杂交,经多代连续选择于2006年秋育成。该组合全生育期118~128d,穗长20cm,每穗总粒数160粒左右,结实率90%,千粒重23g,糙米赤红色,有香味,抗稻瘟病,中感白叶枯病,早、晚稻平均产量为5290.5~7657.5kg/hm2。该组合籽粒种皮着色时间比普通红香稻提早20d左右,成熟收获时糙米粒着色均匀亮丽,解决了普通红香米生产收获时糙米颜色不均匀的问题。 相似文献
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[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823... 相似文献
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