梨树缺铁性黄叶病矫正措施

梨树黄叶病,又称黄化病、白叶病,缺铁性黄叶病。梨树黄叶病起初常见于北方梨产区如东部沿海海滨盐碱地、黄河故道沙荒地和内陆低洼盐碱区,近年来在我国南方梨产区发生也较严重,如四川汉源、湖北平原湖区的潜江、钟祥等地发病严重,有个别梨园黄叶病发病率高达60％～70％,严重影响梨果的产量和品质,也限制了梨产业发展^[1]。     

牛常见难产的矫正

家畜中牛的难产发病率占到首位,由于牛的骨盆比较狭窄,分娩时不利于胎儿通过,加之优良品种的引进,胎儿过大使牛的难产发生率呈上升趋势,因此对牛的难产矫正方法,笔者将多年来在临床实践中一些体会与同行切磋.     

中国农村社区矫正制度研究——以国家和社会的关系为视角

市民社会是社区矫正制度的逻辑起点和赖以形成的社会基础,社会力量的广泛参与是社区矫正制度的本质特征,基于此,市民社会基础的有无就成为一个国家社区矫正工作模式的分水岭。中国农村社会还处于由依附型社会向自治型社会的转型期,远不具有市民社会的风貌,这决定了中国农村社区矫正只能采取＂以政府为主导,以村民自治为依托＂的模式。这一模式意味着中国农村社区矫正应当坚持走群众路线,因此,要顺利推进中国农村社区矫正,不仅要坚持政府主导,在具体对策上还要注重农村非政府组织和志愿者队伍等社会力量的动员、培育。     

浅谈教育的外部性及其矫正

福利经济学自出现以来,就一直关注外部性研究.人们在进行经济决策时也开始考虑外部性的影响,教育领域同样挡不住经济学领域中外部性的强势渗透.教育的外部性会导致教育的福利损失,出于对经济的理性思考,对教育的外部性进行矫正有利于资源的有效配置,提高全社会的福利水平.     

Correction of the Mutant SLA-1-632-TPK Gene from ToPigs Pig and Construction of SLA-1-TPK/pMD19-T Recombinant Plasmid

The swine leukocyte antigen (SLA) genes in pigs were the important immune gene group in antigen presentation, and studing on SLA could provide the references for the prevention of some infectious diseases. Earlier studies found that SLA-1-632-TPK gene in ToPigs pig had a deleted base in its coding sequence (a single base "C" was lost in 632 bp from the 5' end of the SLA-1-632-TPK gene), which lead to frameshift mutation. In order to correct the SLA-1-632-TPK gene, two pairs of gene-correction's primers were designed to correct the gene by the splicing overlap extension PCR (SOE-PCR) in template of recombinant plasmid of SLA-1-632-TPK/pMD18-T. Firstly,the 5'and 3'ends of SLA-1-632-TPK gene were amplified, respectively, then both of them were spliced and amplified to form an intact SLA-1-632-TPK gene. After detected by agarose electrophoresis, the interest of the product was further cloned into pMD19-T Simple vector. The positive clones were screened by colony PCR and then sequenced. The result showed that the 5'and 3' ends of the SLA-1-632-TPK gene were all amplified successfully by SOE-PCR, with the products of about 650 and 590 bp, which were consistent with the theoretical value of 648 and 585 bp, respectively. After spliced, the intact sequence of SLA-1-632-TPK gene was obtained with the product of about 1 200 bp, which was close to the theoretical value of 1 223 bp. The colony PCR result showed that the corrected gene was successfully inserted into pMD19-T Simple vector . After the sequence was analyzed by GENETYX version 9.0, it was shown that the nucleotide "C" in 632 bp numbered from the 5'end of the gene was added and the SLA-1-632-TPK gene was coded correctly. In this study, the SLA-1-632-TPK was corrected successfully, and the recombinant plasmid SLA-1-TPK/pMD19-T was constructed, which would lay a foundation to further study the protein expression and associated function of SLA-1-TPK.     

鸡代谢试验中内源物矫正对测定结果的影响

应用Ｓｉｂｂａｌｄ的真代谢能（ＴＭＥ）法测定了９种蛋白质饲料的能量、氮和干物质代谢率以及经内源物矫正后的能量和氮代谢率。结果表明：能量表观代谢率（Ｅ）与内源能矫正后的能量真代谢诲（Ｅ＇）之间呈高度正相关，且二者与干物质消化率（Ｄ）之间也呈强相关；在部分蛋白质饲料中的表观氮代谢率（Ｎ）与内源氮矫正后的真氮代谢率（Ｎ＇）呈高度正相关；Ｅ与Ｎ＇之间强相关；并导出它们之间的５个回归公式。本次试验预示，用同     

斑马延长蹄削蹄矫正术

斑马在野生条件下,由于在进化过程中所获得的保护机能,蹄角质的生长与磨灭是相适应的,而且这种生长与磨灭的新陈代谢过程,促进了蹄角质的正常生长与蹄的健康。但是,由于在人为条件下饲养,蹄角质的磨灭常失去其自然的平衡,致蹄角质长的很长,甚至变形,影响正常运动及观赏价值。我们于1991年5月,对1例两前肢严重延长蹄的斑马,用削蹄的方法对其进行了矫正,取得满意效果,现将削蹄方法介绍如下。 1 病例介绍长春市动植物园由日本引进的一匹5岁公斑马,体重约400千克。一年前发现两蹄长的较长,     

奶牛子宫捻转的“滚转”疗法

奶牛子宫捻转是奶牛常见的产科病之一,实施"滚转"疗法经济、可行、疗效快。采用剖腹矫正或剖腹产是专业兽医师才能从事的手术,并且剖腹产破坏了牛的子宫结构,影响后期的配种怀孕。对奶农应推广"滚转"疗法。     

托佩克猪SLA-1-632-TPK基因的矫正及重组质粒SLA-1-TPK/pMD19-T的构建

猪白细胞抗原（swine leukocyte antigen,SLA）在猪免疫系统中起递呈抗原作用,对其展开研究可为猪相关传染病的预防提供依据。研究发现,托佩克猪SLA-1-632-TPK基因编码序列发生碱基缺失（距离SLA-1-632-TPK基因5'端632 bp处丢失1个碱基"C"）,导致移码突变。为矫正SLA-1-632-TPK基因,设计2对矫正引物,以原重组质粒SLA-1-632-TPK/pMD18-T为模板,利用剪切重叠延伸PCR（splicing overlap extention PCR,SOE-PCR）技术分别扩增SLA-1-632-TPK 5'和3'端,之后进行拼接,最后扩增全序列从而矫正目的基因,并进一步连接pMD19-T Simple载体,通过单菌落PCR筛选阳性克隆并测序,并通过GENETYX version 9.0软件对所测序列进行分析。结果显示,SOE-PCR成功扩增得到5'和3'端片段,大小约为650和590 bp,与理论设计值大小（648和585 bp）接近,经过拼接以后,得到全长约1 200 bp,与理论设计值1 223 bp接近。菌落PCR结果显示,矫正基因成功克隆入pMD19-T Simple载体。序列分析结果显示,托佩克猪SLA-1-632-TPK基因距离5'端632 bp处丢失的碱基"C"得到矫正并正确编码。本研究成功矫正了SLA-1-632-TPK基因,并构建其重组质粒SLA-1-TPK/pMD19-T,为下一步研究SLA-1-TPK蛋白表达和相关功能奠定基础。     

胎儿前腿姿势异常引起母畜难产的诊治

1腕关节屈曲两侧腕关节屈曲时,事先如未拉过头部,在阴门处什么也看不到,单侧性腕关节屈曲时,可见到屈曲的腕关节摆在骨盆入口处,阴门处可见到另一伸直的前腿。产道检查时,可摸到一或二侧屈曲的腕关节位于耻骨前缘附近,或揳入骨盆腔内。