上海市犬只狂犬病认定免疫点主要管理做法分析

强化犬只狂犬病免疫工作是从源头控制狂犬病的最有效措施.近年来,上海市按照《上海市养犬管理条例》的要求,通过严把免疫资质准入、规范内部管理、落实疫情报告责任、推进免疫质量考核等举措,使犬只狂犬病认定免疫点的布局日趋合理,文明服务更加规范,这已成为上海市民犬只狂犬病免疫的主渠道,免疫数量和质量逐年提升,构筑了良好的免疫屏障...     

通径分析新探索

对通径分析中自变量个数选择、剩余决定系数评价及引入自变量显著标准提出了新见解,提出了逐步回归通径分析法。     

基于灰色关联分析方法的区域土地利用变化研究

通过分析其土地利用现状特点、土地利用中存在的问题,采用灰色关联分析方法,找出区域土地利用变化的主要因素,并对土地资源可持续利用提出建议。     

花生AT-hook家族基因的生物信息学分析

AT-hook蛋白不仅在植物生长发育、器官构建、胁迫和激素信号应答中起重要作用,而且还作为染色质重塑的转录因子和辅助因子,调节基因的转录活性.为全面了解花生AT-hook基因家族的结构特征,利用生物信息学技术比对花生基因组数据库,分析AT-hook基因家族成员的理化性质、基因结构、保守结构域和系统发育关系以及在12个组...     

草鱼SGLT1/2基因克隆及葡萄糖处理对其mRNA表达的影响

作为重要的转运载体,SGLTs(钠依赖性葡萄糖协同转运蛋白,sodium-glucose co-transporters)在物质的吸收过程中发挥着关键的作用.SGLT1和SGLT2作为SGLTs家族的重要成员,参与调节体内的葡萄糖吸收,从而维持血糖的稳态.为研究SGLT1和SGLT2在草鱼葡萄糖吸收过程中的作用,本实验...     

Detection of arboviruses in Culicoides(Diptera: Ceratopogonidae) collected from animal farms in the border areas of Yunnan Province,China

Biting midges of the genus Culicoides(order Diptera, family Ceratopogonidae) are potential biological vectors for the transmission of certain arboviruses among humans, livestock, and wild animals. This study collected a total of 405 Culicoides individuals from seven animal farms located in five counties in the border areas of Yunnan Province, China, and examined the Culicoides species composition and the major arboviruses carried by the Culicoides species. The collected Culicoides were classified into seven species with variable abundances: Culicoides arakawae(5.43%, 22/405), Culicoides homotomus(1.23%, 5/405), Culicoides obsoletus(19.75%, 80/405), Culicoides orientalis(17.28%, 70/405), Culicoides oxystoma(29.38%, 119/405), Culicoides peregrinus(5.68%, 23/405), and Culicoides nipponensis(21.23%, 86/405). Among the seven species, C. oxystoma and C. nipponensis were distributed in all the five counties with abundances of 13.33–44.87% and 10.00–46.83%, respectively, suggesting that these were the dominant species of Culicoides widespread on animal farms in the border areas. PCR was used to detect major arboviruses in the collected Culicoides specimens, including bluetongue virus(BTV), Japanese encephalitis virus, Dengue virus, Zika virus, African swine fever virus, and African horse sickness virus. Among the tested viruses, only BTV serotype 1 was tested positive in C. oxystoma specimens collected from a buffalo farm. Culicoides oxystoma was the dominant species on animal farms in the sampled areas, but it has not previously been documented as positive for BTV in China. The current results thus suggest that C. oxystoma could be an important vector for BTV transmission in these border areas, which, however, needs to be confirmed by further comprehensive experiments. Overall, the present study provides the first profile of Culicoides species on animal farms in the China, Vietnam, and Myanmar border areas, establishes the prevalence of arboviruses carried by these Culicoides species, and suggests the vector potential of C. oxystoma species for the transmission of BTV.     

青海云杉移植苗不同施肥方法生长效益分析

通过不同施肥方法试验,研究有机肥、化肥及有机肥与化肥配施对青海云杉移植苗生长的影响。结果表明,施用化肥、有机肥均能促进青海云杉移植苗生长,而有机肥与N、P、K肥配施,才能获得更高的生长量。     

雷竹覆盖栽培失败原因浅析

总结了嵊州市雷竹覆盖栽培技术推广应用经验,对嵊州市的雷竹覆盖栽培失败典型事例进行了认真的剖析,提出了嵊州市雷竹覆盖栽培的对策与措施和4点建议。     

Preliminary Research on Regional Material Flow Analysis： A Case Study of Chengyang District in Qingdao

The method ＂Material Flow Analysis （MFA）＂ is one of the effective tools to study law and quantification of material flow between economic system and ecological system. On the national level, economy-wide material flow analysis has been published for a number of countries. However, published studies on the regional or local level are still very limited and a standardized method does not exist yet. On the basis of framework of Material Flow Analysis proposed by Eurostat, the paper collected related data and analyzed material input and output for the Chengyang district in Qingdao. The results showed that DMI （Direct Material Input） and TMR （Total Material Requirement） in absolute number increased about 3.6 and 3.9 times, respectively from 1995 to 2004. Fossil fuel and mineral contributed to about 50.3%-76.3% of DMI. Imports of material increased about 5.0 times and became the most important Contributor to DMI, which showed that local economic growth was highly dependent on resources from other regions and countries. DPO （Domestic Processed Output） and TDO （Total Domestic Output） represent slow increasing trend, and DPO contributed to 22.2%-58.1% of TDO, suggesting local hidden flows were of obvious effect on TDO. The biggest component of DPO is CO2, approximately accounting for 90% of DPO. The material productivity increased 57.7% in last decade, reflecting improvement of efficiency of resources utilization in some extent. However, compared to developed countries and regions, material productivity of Chengyang district was relatively low. Therefore, in order to promote the sustainability, it is essential to develop circular economy and enhance materials productivity.     

不同贮藏温度对磴口华莱士蜜瓜质构特性、理化指标、感官品质变化及相关性分析

以磴口华莱士蜜瓜为试材,研究不同贮藏温度对其采后果实质构特性、理化指标、感官品质变化及相关性分析。选用成熟度一致的果实随机分为2组,放置于常温(28℃)和低温(6℃)条件,每隔1 d进行感官评定,常规理化指标检测、质地多面分析(TPA)及整果穿刺试验。结果显示,低温贮藏能有效减缓果实可溶性固形物和有机酸含量的下降,有效延缓果皮硬度、果肉硬度和咀嚼性等的下降,并抑制果实的软化,较常温贮藏延长贮藏期12 d。相关性分析表明,质构参数之间、质构参数与感官品质指标之间存在不同程度的相关性。综上所述,磴口华莱士蜜瓜果皮硬度、咀嚼性和内聚性等均能较好地反映果实品质的变化,可作为其品质评价指标;该结果为开发磴口华莱士蜜瓜的贮藏保鲜技术提供了科学依据。