Regulatory Network of Transcription Factors in Response to Drought in Arabidopsis and Crops

Drought is one of the most important environmental constraints limiting plant growth, development and crop yield. Many drought-inducible genes have been identified by molecular and genomic analyses in ...     

祁连山林区土壤水分条件的分析与评价

通过对祁连山林区云杉林、圆柏林、灌丛林和牧草地等4种主要植被类型土壤水分动态进行长期定位研究,揭示出生长季节内各植被类型的土壤水分动态变化规律.从土壤水分的变化特征,土壤水分有效性等几个方面对祁连山林区主要植被类型的土壤水分条件进行了分析与评价,表明祁连山林区主要植被类型的土壤水分垂直变化可分为土壤水分弱利用层、土壤水分利用层、土壤水分调节层;林地土壤水分的季节变化可分为土壤水分消耗期、土壤水分补偿期、土壤水分消退期、土壤水分稳定期4个时期.根据土壤水分对植物的有效性,将土壤水分划分为易效水、中效水和难效水三种状态,评价土壤水分有效性认为云杉林水分供应状况最好.     

Conditional expression of harpinPsscauses yeast cell death that shares features of cell death pathway with harpinPss-mediated plant hypersensitive response (HR)

Conditional expression of harpin_Psscauses yeast cell death that shares features of cell death pathway with harpin_Pss-mediated plant hypersensitive response (HR).Pseudomonas syringae pv.syringae 61 hrp Z gene encodes harpin_Pss, a 34.7 kD extracellular protein that elicits a hypersensitive response (HR) in plants. Conditional expression of either full-length or truncated hrp Z sequences under the GAL1 promoter caused cell death in Saccharomyces cerevisiae Y187. Plating of pYEUT- hrp Z transformants on a medium containing galactose resulted in complete inhibition of colony formation, whereas their growth on a glucose-based medium was unaffected. Western blot analysis confirmed the expression of harpin_Pssin yeast cells transformed with pYEUT- hrp Z and grown in galactose-containing medium. A time-dependent decline in the percentage of trypan blue-excluding cells in cultures of pYEUT- hrp Z transformants was observed when cultured on galactose-containing medium. Similarly, the number of viable cells reduced to about 50% within 6 h. There were similarities in the harpin_Pss-mediated cell death in plants and yeast cell death (YCD). Galactose-induced cell death in pYEUT-hrp Z transformants of S. cerevisiae Y187 was suppressed by a protein kinase inhibitor K252a (10 μ M). The viability of pYEUT- hrp Z transformants was prolonged in the presence of 100 U ml⁻¹catalase suggesting a role for the oxidative burst in YCD that was further supported by the flow cytometric patterns of propidium iodide uptake by yeast cells. Overall, it appears that yeast provides a useful model system to understand the molecular mechanism of harpin_Pss-mediated cell death.     

中国与欧美农业科技创新体系对比研究

农业科技创新发展已成为推动现代农业发展的重要原动力。近年来,中国步入农业科技创新发展的快车道,为探寻建立健全中国农业科技创新体系的路径与方法,对美国、英国、德国与中国的农业科技创新体系进行对比。结果表明：①农业产业的特殊性决定农业科技创新体系的特殊性,农业科技创新主体、农业科技创新环境、农业科技创新成果与应用、农业科技创新国际合作相互作用,构成完整的农业科技创新体系。②产学研紧密结合是美国农业科技创新体系的突出特点,英国则是注重政府引导、信息共享与知识服务,德国是信息化生态型农业科技创新体系代表,注重信息引领与绿色、可持续发展。③与欧美农业科技创新体系相比,我国农业科技创新体系仍存在一些不足,未来可从协调统筹、规划建设、队伍与机制建设等方面入手,全面推动我国农业科技创新体系发展建设。     

试析转型期文学的发展

二十世纪90年代,世界格局发生了根木的变化,人类试图在“全球一体化”的语境中寻求共同的利益。经济领域计划经济向市场经济的转型势必会引起上层建筑中文化、意识的转型。转型期的中国当代文学呈现出现代主义”与后现代主义”文化语境互相渗透,知识分子人文意识的变化与失落,文学创作的精神属性淡化与商品生产强化的特点。     

Studies of clomazone mode of action

Intact spinach chloroplasts were used to determine if clomazone, 5-OH clomazone, and/or 5-keto clomazone inhibited the chloroplastic isoprenoid pathway. When isopentenyl pyrophosphate was used as a precursor, neither clomazone nor the clomazone metabolites (5-OH clomazone and 5-keto clomazone) inhibited the formation of products separated by HPLC in the organic phase. However, when pyruvate, a substrate for the first committed step of the pathway, was used as a precursor, both 5-keto clomazone and fosmidomycin reduced the formation of a non-polar product and increased the formation of a polar product in the organic phase. Only 5-keto clomazone, not 5-OH clomazone or clomazone, inhibited the formation of an additional product other than fosmidomycin in the aqueous phase from pyruvate incorporation. In an in vitro assay, 5-keto clomazone inhibited DXP synthase, the enzyme catalyzing the first committed step of the chloroplastic isoprenoid pathway. Therefore, our studies show that neither clomazone nor 5-OH clomazone inhibits the chloroplastic isoprenoid pathway, only 5-keto clomazone does.     

When, why, and how to perform cardiac radiofrequency catheter ablation

Radiofrequency catheter ablative techniques provide a unique opportunity to cure dogs of certain supraventricular tachyarrhythmias, rather than simply controlling the rhythm with drug therapy. Accessory pathways, which can participate as one limb of a rapid, narrow complex tachyarrhythmia circuit, have a particularly high cure rate with radiofrequency catheter ablation. Focal atrial tachycardias also provide a substrate that can be amenable to ablation. Tachycardia-induced myocardial dysfunction resulting from frequent tachyarrhythmic episodes will resolve after a successful ablation. This report outlines the indications, equipment, and techniques used in dogs for performing catheter ablation with temperature-controlled radiofrequency energy delivery.     

套袋对苹果果皮花青苷合成及着色的影响

套袋是提高苹果果实着色的主要技术措施之一,也是研究苹果果皮花青苷合成和基因表达的重要手段。苹果果皮花青苷的合成经由苯丙烷类代谢途径,编码苹果花青苷合成酶类的基因已得到克隆。套袋后果皮花青苷合成酶类的基因表达受到抑制,因而抑制了花青苷合成;摘袋后果皮花青苷迅速合成,与套袋后果皮光受体浓度增加和叶绿素含量降低从而提高对光的敏感度、迅速启动花青苷合成酶类的基因协同表达有关。果皮色素特别是花青苷和叶绿素的含量、比例和分布决定果实的着色状况,套袋促进果皮花青苷合成的同时大大降低了叶绿素含量,从而使果实着色鲜艳。     

根癌农杆菌介导Cry1Ac基因转化‘雪青’梨获得转基因植株

 以‘雪青’梨叶片愈伤组织为外植体, 经根癌农杆菌介导将CaMV35S启动子调控下的Cry1Ac基因导入‘雪青’梨。698块愈伤组织和231个Kan^r芽与携带表达载体质粒pBX203的根癌农杆菌菌株LBA4404共培养3 d后, 转入含50 mg·L^-1 Kan的筛选培养基培养30 d, 15 d转接1次。结果表明,在MS + 5 mg·L ^-1 6-BA + 0.1 mg·L^-1 IAA筛选培养基中, Kan^r芽率为34.24% , 在1/2MS + 2 mg·L^-1 IBA+ 0.5 mg·L^-1 6-BA + 500 mg·L^-1AC筛选培养基中, 15.58% Kan^r芽生根。共获得再生植株32株, 经PCR和Southern-blot分析证明, 其中4株的基因组已成功导入和整合Cry1Ac基因。     

花生幼苗不同切断及其愈伤组织的农杆菌介导转化

试验进行了不同花生外植体（成熟胚的幼叶、胚轴、子叶等）的直接农杆菌转化与花生愈伤组织的农杆菌转化,比较其感染后的诱芽与上分化生长情况,并对影响农杆菌介导转化花生的因素进行了探讨.结果表明农杆菌的生长状况、浓度及侵染时间和筛选剂的浓度是影响农杆菌介导花生转化的主要因素;褐化是影响其转化的关键问题.