磷肥在褐土中的动态转化及施用有机肥的影响

利用培养试验研究磷肥施入褐土后的动态转化以及施用有机肥对其转化的影响，结果表明：施肥初期，磷肥主要转化为Ｃa2-P,但随着时间的延续，很快的Ｃag-P、Al-P转化。施用有机肥对化学磷肥的率有较大影响。     

数字化地籍图数据处理方法的研究

本文系统、全面地讨论了数字化地籍图数据处理的各种方法、特性及其应用范围。并结合实例对利用已知面积校正数字化坐标的平差计算方法作了详细的讨论。通过一系列的数据处理 ,可以向地理信息系统提供精度可靠的地理基础数据。     

转型时期有关农民职业教育问题研究的动向

通过对转型时期有关农民职业教育问题的研究动向的分析,指出研究者对此问题的研究呈现出明显的动态性特征.具体表现在以下5个方面:①逐步形成了初具规模的研究群体;②研究领域不断拓展;③研究的时代性特点日益明显;④研究逐步由表象深入到本质;⑤研究逐渐体现以人为本的科学发展观.     

贺兰山主峰哈拉乌沟流域三水转化关系探讨

贺兰山主峰哈拉乌沟流域位于阿拉善左旗巴音浩特镇东侧,沟内1977年为旗镇建截潜流供水水源地。根据1979年~1987年实际观测沟域降水,地表水和潜流资料,研究提出三水转化参数,可为内蒙古半干旱山丘区开发利用沟谷潜水进行水资源评价参考。     

发根农杆菌(Agrobacterium rhizogenes) Ri T-DNA遗传转化丝石竹(Gypsophila paniculata)的研究

近年来,已发展出遗传转化高等植物的一些新技术,其中有些技术如脂质体融合,微注射技术和电击导入都是基于动物细胞培养方面的工作,而另一些技术是来自于植物界独特的天然转化系统,其中包括已知能遗传转化高等植物的农杆菌(Agrobacterium)的二个种,即致瘤农杆菌(A.tumefaciens)和发根农杆菌(A.rhizogenes),这二个种都能够将其致病质粒Ti或Ri所携带的DNA序列(T-DNA)插入到双子叶植物细胞核基因组中。pTi诱发寄主产生根基肿瘤,pRi诱发寄主产生毛状根。二者的差别可能是毛状根可以从毛状根培养物获得具有完整的T-DNA序列的有生育能力的再生植株,而从致病农杆菌(A.tumefaciens)菌株所诱发的肿瘤很难获得再生植株。因此,利用发根农杆菌(A.rhizogenes)pRi作为遗传转化高等植物的基因克隆载体的研究和应用日益受到重视。     

落叶松体细胞胚胎发生研究进展

综述了欧洲落叶松、日本落叶松、欧日和日欧杂种落叶松、西部落叶松和华北落叶松体细胞胚胎发生的研究进展，对胚性培养物的超低温保存、原生质体培养和基因转化等技术进行了介绍，并对落叶松体细胞胚胎发生的研究趋势作了展望。     

厦门城市森林马尾松宽带更新改造技术研究

厦门城市森林中马尾松劣质林分比例大,采用不同改造方式试验表明:宽带采伐更新改造效果较好,阔叶树种造林成活率高,幼林生长比较快,适宜阳性、强阳性和中性偏阳树种。且宽带更新改造技术较简单,可操作性强,便于推广应用。     

Liquid culture and transformation of hinoki cypress (Chamaecyparis obtusa Sieb. et Zucc.)

Hinoki cypress (Chamaecyparis obtusa) is one of the most important timber resource forest trees in Japan. Because seed production from a seed orchard of hinoki cypress is not constant every year, micropropagation from a limited amount of material is useful. Up to now, the conventional tissue culture method using solid medium has been used. Here a new method using liquid culture in tubes rotated vertically is described. Shoot primordium of hinoki cypress was inoculated in Campbell and Durzan’s (CD) liquid medium containing different cytokinins (6-benzylaminopurine (BAP), Zeatin, thidiazurone (TDZ)), and the container tubes were rotated vertically around the axis at 2 times / min. Culture room temperature was 25°C and light condition was 16 h photoperiod per day of fluorescent lamps. Zeatin at 1μM concentration was the best for maintaining the shoot primordium production and TDZ induced callus on the surface of the shoot primordia. After shoot primordium multiplication in the liquid culture, they were transplanted to agar medium for shoot elongation. A high concentration of agar (up to 16 g/L) or AVF (anti vitrification factor from Dr. Nairn, 1995) was effective to prevent vitrification of the shoots. Transformation of shoot primordium was done using particle bombardment with vectors containingβ-glucuronidase (GUS) gene or herbicide resistance gene (bar). Positive result for transient transformation was observed with the histo-chemical study for transformation with GUS. Integration of a useful herbicidebar gene into the shoot primordium culture system was also tried and stably transformed plants were obtained. This is the first report of stable transformation of Japanese conifer using practically useful gene. The generous supply of AVF-B from Dr. B.J. Nairn, Tasman Forestry, NZ is also appreciated.     

One-day rate measurements for estimating net nitrification potential in humid forest soils

Measurements of net nitrification rates in forest soils have usually been performed by extended sample incubation (2–8 weeks), either in the field or in the lab. Because of disturbance effects, these measurements are only estimates of nitrification potential and shorter incubations may suffice. In three separate studies of northeastern USA forest soil surface horizons, we found that laboratory nitrification rates measured over 1 day related well to those measured over 4 weeks. Soil samples of Oa or A horizons were mixed by hand and the initial extraction of subsamples, using 2 mol L⁻¹ KCl, occurred in the field as soon as feasible after sampling. Soils were kept near field temperature and subsampled again the following day in the laboratory. Rates measured by this method were about three times higher than the 4-week rates. Variability in measured rates was similar over either incubation period. Because NO₃⁻ concentrations were usually quite low in the field, average rates from 10 research watersheds could be estimated with only a single, 1-day extraction. Methodological studies showed that the concentration of NH₄⁺ increased slowly during contact time with the KCl extractant and, thus, this contact time should be kept similar during the procedure. This method allows a large number of samples to be rapidly assessed.     

国有林业产业对地区经济发展贡献度的动态演绎分析

基于1998~2018年黑龙江省国有林业产业数据,从发展规模、产业结构、投入产出效益、专业化水平等4个方面对黑龙江省国有林业产业发展现状进行总体概述和分析。借助产业贡献度指标测算国有林业产业对黑龙江省经济发展的贡献水平,并基于三次产业构成进一步对国有林业产业贡献度进行了结构性动态评价。研究得出:黑龙江省国有林业产业对地区经济发展贡献呈现稳步上升的特征,第一产业表现突出,第二、三产业呈小幅下降、小幅上升的反向变动特征。基于结果分析,从产业自身发展和持续推进多种经营产业转型两个方面提出方向性建议。