不同防虫网覆盖对网室小气候及青菜生产的影响

以日本早生华京青菜为试材,采用立柱框架式网室栽培,研究了18目、22目银灰色防虫网覆盖对网室内温度、湿度、光照等小气候及青菜生长发育的影响.结果表明,在立柱框架上覆盖防虫网对网室内气温、湿度、光照等小气候均有不同程度的影响,其中网室内气温、湿度与露地相比增加,晴天和阴天的湿度增幅较明显,而温度增幅不明显;网室内光照则明...     

猪粪堆肥过程中NH3和H2S的释放及除臭微生物的筛选研究

为了治理粪便臭气污染，测定了猪粪好氧堆肥过程中NH₃和H₂S的释放量以及堆肥温度、pH值、含水率、水溶性铵态氮等指标。结果表明，NH₃在前20天释放量占总释放量的84.6%、H₂S在前13天释放量占总释放量的100%。因此，在堆肥初期的前20天是控制臭气的最佳时期。并从畜禽粪便、垃圾、土壤和堆肥中分离、纯化了一些微生物，经过初筛得到能利用NH₃的微生物41株，经过复筛得到脱除H₂S较好的细菌一株，与对照相比其去除率达85.7%，经鉴定该菌是松鼠葡萄球菌。     

大功率UPS负载试验的数据采集与监视系统设计

提出并设计了大功率UPS负载试验系统及其数据采集与监视模块，讨论了该系统的原理及硬件结构和软件设计。该系统可测量UPS三相电压、电流、功率、频率和相位等参数，为大功率UPS电源的测试提供了有力的工具和实验手段。实验结果表明，该系统性能稳定、工作可靠。     

花粉管通道法转基因棉花后代筛选鉴定

通过对棕色棉BC05叶片进行Kan浓度梯度涂抹实验,确定了Kan涂抹筛选的最佳浓度为3.0 g/L,最佳观察时间为涂抹第5天。对Kan抗性植株进行PCR鉴定表明,Kan涂抹筛选可基本准确的鉴别出转基因材料与非转基因材料,但目标基因是否成功整合还必须进行PCR鉴定,田间卡那霉素筛选结合PCR分子鉴定是转基因棉花后代大规模群体筛选的有效方法。     

分子印迹固相萃取和丝网印刷电极联用技术检测猪尿中盐酸克伦特罗

实验采用本体热聚合的方式合成盐酸克伦特罗的分子印迹物(molecularly imprintedpolymers,MIPs),通过扫描电镜、吸附实验和分子印迹固相萃取研究该印迹材料的性能。用盐酸克伦特罗分子印迹材料作为固相萃取剂,优化固相萃取条件,采用差分脉冲伏安法(DPV)对洗脱液进行电化学性能测试。结果表明,此印迹物的平衡吸附常数(b)为5.14mL/mol,电化学传感器的电流值与盐酸克伦特罗的浓度在0.056～2.78mg/L浓度范围内呈现良好的线性关系,检测下限为0.005mg/L,盐酸克伦特罗在猪尿样品中的回收率为89.3%～99.6%。此萃取方法也呈现出良好的选择性和稳定性。     

基于ZY-1-02C卫星遥感图像的森林采伐情况分析

以广西南部某县部分区域为研究区,采用ZY-1-02C卫星遥感图像为数据源,通过目视解译、屏幕矢量化方法,提取2012年实际采伐的伐区空间分布信息,在AreGIS平台支持下,与2012年伐区调查图进行空间叠置分析,对2012年实际采伐情况分析.结果表明：2012年实际采伐伐区共396个,总面积为4 557.8 hm2,其中有证采伐伐区267个,占总伐区数的68.4％,面积3 823.4 hm2,占总面积的83.9％;无证采伐伐区129个,占总伐区数的31.6％,面积734.4 hm2,占总面积的16.1％.在有证采伐伐区中,面积总误差为2.7％,总体重叠率为82.0％,其中超界采伐712.2 hm2,少采607.2 hm2.说明该研究区森林采伐中,无证采伐情况较为严重,即使是有证采伐,也存在部分伐区未严格按设计要求进行采伐的情况.     

Effect of topical anesthesia on evaluation of corneal sensitivity and intraocular pressure in rats and dogs

Objective To determine the effect of 0.5% proparacaine in tonometry by evaluating corneal touch threshold (CTT) and intraocular pressure (IOP). Animal studied Nine rats (18 eyes, Sprague–Dawley) and 10 dogs (20 eyes, Beagle) Procedures The IOP and CTT were measured in each eye before and after topical anesthesia with 0.5% proparacaine. The IOP was evaluated using Tonopen for dogs and Tonolab for rats. The corneal sensitivity was evaluated by CTT through a Cochet–Bonnet aesthesiometer. Results The mean IOP was not significantly changed in rats or dogs before and after topical anesthesia. However, after application of proparacaine, CTT was significantly increased in both animal groups compared with that before application of proparacaine. Conclusion From this study, topical anesthesia was found to significantly lower the corneal sensitivity but have little effect on IOP measurements. In ophthalmologic examination, topical anesthesia can be used to reduce corneal sensation without an effect on IOP.     

Correlation between corneal sensitivity and quantity of reflex tearing in cows,horses, goats,sheep, dogs,cats, rabbits,and guinea pigs

Objective Guinea pigs have a very low threshold of corneal sensitivity and at the same time nearly no reflex tearing compared to dogs, cats, and horses. The question arose whether there is a general correlation between corneal sensitivity and the quantity of reflex tearing. Animals studied Totally 160 animals of 8 different species (20 animals per species) were investigated. Procedures The corneal touch threshold (CTT) was measured with a Cochet–Bonnet esthesiometer. The palpebral fissure length (PFL) was measured with a calliper ruler. The Schirmer tear test (STT) was modified by adapting the width of the STT strip to the PFL of every species. For the STT II, 0.4% oxybuprocaine was applied. Results Corneal touch threshold: Cows (1.67 g/mm²), horses (1.23 g/mm²), sheep (1.13 g/mm²), goats (1.44 g/mm²), dogs (2.16 g/mm²), and cats (1.33 g/mm²) show similar CTT values. In contrast, rabbits (6.21 g/mm²) and guinea pigs (7.75 g/mm²) show a significantly lower CTT. Tear Production Difference STT I ? STT II: Rabbits have the greatest decline in tear production with 38.4%, followed by sheep (33.3%), dogs (31.1%), cats (24.7%), cows (23.7%), horses (18.0%), and goats (14.0%). Guinea pigs have no decline, but a slight increase of ?16.0%. Correlation CTT and STT II ? STT I Difference: Pearson’s correlation coefficient shows a small, but significant correlation. The coefficient of determination can only forecast a value with 7.1% certainty. Conclusions The high variance and low reproducibility of results suggest that the measuring devices are inappropriate to assess the evaluated parameters. Therefore, no assured correlation between the corneal sensitivity and the quantity of reflex tearing could be found.     

水稻优质高产新品种筛选试验

为加快六安市水稻品种更新换代,加大优良水稻新品种展示及示范推广力度,特引进一批适宜长江中下游地区种植的优质高产中籼稻新品种,综合考察其生育期、产量性状、抗性、加工品质及外观品质等。结果表明：荃优香66、玮两优8612和荃优鄂丰丝苗熟期适中,抗性好,可作为优质高产水稻主推品种规模化种植;荃优香66外观品质较佳,还可发展订单生产。     

河西地区适宜种植水果萝卜品种的筛选

【目的】在河西地区引进 8 种国内优质的水果萝卜,筛选适宜当地种植的水果萝卜品种,为新品种选育、种质资源创新和新品种推广提供理论依据。【方法】选取适宜鲜食的 8 种水果萝卜品种（七星、华懿水果萝卜、潍县萝卜、串心红、苹果脆、申萌脆梨、醉红和君川景福,依次编号为T1~T8）为试材,测定叶长、叶宽、最大展幅、地上部根长、根粗,气孔导度（G_s）、净光合速率（Pn）、胞间CO₂浓度（C_i）、蒸腾速率（T_r）、叶绿素含量SPAD值、水分利用效率（WUE）,以及可溶性固形物、维生素C、可溶性糖、粗纤维含量和产量等指标,结合感官评价法对水果萝卜品质进行综合评价。【结果】8种水果萝卜出苗率有差异,出苗最早的为T2;8种水果萝卜果实发育时间为40~48 d,生长势和整齐度表现良好;植株生长性状存在丰富的多样性,T7地上部根最长,T2根最粗,T7叶长和叶宽显著高于其余品种（T6、T8叶宽除外）;T2叶片P_n、G_s、C_i、T_r、SPAD表现最好;WUE呈先升后降的趋势（除T8外）;不同品种风味品质的感官评价综合得分为15.50~22.31,T2最高;不同品种可溶性固形物含量为7.06~10.29 mg/g,维生素C含量为9.75~15.23 mg/hg,可溶性糖含量为4.83~9.16 mg/g,粗纤维含量为12.27%~18.87%;T2的单根质量、小区产量和单位面积产量最高,较T7分别显著增加73.00%,55.05%和54.21%。【结论】引种河西地区后,8种水果萝卜品种农艺性状和果实品质表现出丰富的多样性,其中T2的综合得分和产量最高,适宜作为河西地区水果萝卜种植及种质创新的材料。