柚木离体快速繁殖技术

从优良柚木林段选取3~4a生优良单株,从中选取无病虫害的幼嫩侧芽,经表面消毒后接种到无菌培养基上,侧芽萌发率达70%~80%。切取新芽进行增殖,30~40d为一周期,增殖系数为2~3倍。当侧芽增殖到足够数量时,转移到生根培养基上,生根率超过80%~90%。植株移栽成活率达80%~90%。几个月后植株便可种植到大田。      

苜蓿褐斑病的离体叶接种研究

研究和鉴定苜蓿褐斑病抗病性的离体叶接种技术。结果表明,离体叶接种鉴定比传统的整株鉴定效率高,结果可靠。在0.3%水琼脂培养基中加入50μm/mL苯骈咪唑,即可达到最佳保绿效果。接种所需的适宜条件是,相对湿度接近100%,温度20℃,接种时间20h,无光照。培养所需的适宜条件是,生长箱内光照(光强度9000lux)和黑暗每12h交替一次,有光时22℃,无光时18℃。     

金刺塔仙人掌快速繁殖技术研究

以金刺塔仙人掌嫩茎为外植体进行离体培养 ,茎段的最高分化率为 87%。在改良的MS(氮元素减半 ) +BAP1mg/L +IAA0 .2mg/L平均每个茎段可诱导不定芽 9～ 1 3个。在生根培养基中 ,以MS培养基 (氮元素减半 )加NAA0 .2mg/L ,根诱导率在 91 %。当苗高 3cm以上 ,根长 2cm以上时 ,开盖 2d移入温室 ( 2 0～ 2 6℃ ) ,炼苗 3d后移栽于蛭石 :河沙 :腐殖土为2 :3:5的混合基质的土壤中 ,成活率达 89%。     

四倍体刺槐优良无性系间组织培养比较

通过对四倍体刺槐K2、K5无性系芽增殖基本培养基和激素配比比较试验研究,其结果表明:K2、K5无性系芽增殖各指标在不同培养基、不同激素浓度配比情况下生长趋势表现均基本一致;K2、K5无性系适宜的基本培养基为MS;芽增殖最佳激素配比为:6-BA1.0 mg/L+NAA0.5 mg/L+IBA 0.5 mg/L.     

香叶组培快速繁殖的研究

以香叶天竺葵1年生茎段为外植体建立无菌苗,用无菌嫩茎、嫩叶在MS基本培养基附加低浓度的细胞分裂素和生长素上诱导愈伤组织,经过分化和增殖培养获得无根苗,在含有较低无机盐浓度和生长素水平的生根培养基上使其生根,生根率高达100.试管苗移栽到河沙与腐殖质混合的基质中,成活率达95以上.     

Empirical Models for Predicting the Dry Matter Yield of Grass Silage Swards Using Plant Tissue Analyses

Quantifying spatial variability in forage grass yield within individual fields is hampered by the lack of accurate yield monitoring equipment. Here, it is shown how dry matter (DM) yield of silage swards can be predicted on the basis of their mineral composition. This empirical method of predicting yield enables diagnoses of sward nutrient status to be made simultaneously from the tissue test information, and provides a unique opportunity for identifying the nutritional and non-nutritional factors responsible for variability in sward productivity at sub-field scales. Maps of sward DM yield at first, second and third cut silage stages in 1999, and at first cut silage stage in 2000, on a large (7.9 ha) grassland field were produced using two different yield models: one model for first cut and a separate model for second and third cuts. The maps indicated that DM production varied considerably across the field, particularly at first cut, but that the pattern of yield variability at this cut was consistent from 1999 to 2000. The results of the plant tissue tests suggested that N deficiency had been responsible for limiting DM production on the lower yielding parts of the field.     

Concentration effects of dicamba on shoot regeneration in wheat

Shoot formation and regeneration rates of calli derived from immature embryos of three spring wheat varieties were investigated to evaluate the influence on wheat regeneration of different concentrations of dicamba (0.5, 0.1 and 0.02 mg/l) in the regeneration medium. It was found that dicamba concentrations much lower (0.02 and 0.1 mg/l) than the levels reported previously (1 and 0.5 mg/l) induced significantly more shoots per callus in all three varieties, while the enhancement in regeneration rate was variety dependent. Overall, a dicamba concentration of 0.02 mg/l was more favourable for wheat regeneration.     

网状内皮增殖病病毒(REV)感染SPF雏鸡后局部免疫组织抗体生成细胞的变化

对 SPF雏鸡人工感染 REV后局部免疫组织盲肠扁桃体和哈德氏腺的 Ig A、Ig G和 Ig M抗体生成细胞数量动态变化进行了检测。结果发现 ,REV感染 SPF雏鸡后 7～ 4 9天消化道局部免疫组织盲肠扁桃体和呼吸道相关局部免疫组织哈德氏腺的 Ig A、Ig G和 Ig M抗体生成细胞数量明显低于未感染对照雏鸡。表明雏鸡感染 REV后消化道局部组织盲肠扁桃体和呼吸道相关免疫组织哈德氏腺体液免疫机能均显著下降。     

酶制剂对雏鹅组织中胰岛素样生长因子的影响

18只 7日龄的杂交雏鹅 ,随机分成试验和对照两组 ,饲以大麦基础日粮 ,试验组日粮中添加 0 .1%的Biokyowa酶制剂。测定 2 1日龄血液、肝脏和肌肉中的IGF -I含量。添加粗酶制剂后 ,肝脏和肌肉中的IGF -Ⅰ分别较对照高18.2 3% (P <0 .0 1)和 2 2 .79% (P <0 .0 1) ,而血浆中IGF -I水平却较对照低 9.0 4 % (P <0 .0 5 )。结果提示 :酶制剂能促使肝脏合成IGF -I,血液中与IGFBP结合的IGF -I大量释放 ,从而显著提高肌肉中的IGF -I水平 ,加速了肌肉的生长。     

花椰菜尾菜发酵饲料对保育猪生长性能、血清生化指标、小肠组织形态及经济效益的影响

为研究花椰菜尾菜发酵饲料对保育猪生长性能、血清生化指标、小肠组织形态及经济效益的影响.选取42头体重相近(11.40±1.36)kg、体况良好的40日龄保育猪,随机分为两组,每组3个重复,每个重复7头.对照组饲喂基础饲粮,试验组饲喂由80％基础饲粮和20％花椰菜尾菜发酵饲料组成的试验饲粮.试验共30 d,其中预试期8 ...