十二个李品种的花粉形态的研究

应用电子扫描显微镜,对12个李品种的花粉进行了形态观察.结果表明:12个品种的花粉均属于中等大小的花粉(25～50 μm),花粉粒两端均为圆弧状.一些李品种的花粉外部形态差异明显,赤道面观分别呈长球形和扁球形之分,扁球形花粉的萌发孔在赤道面凸出角端;极面观有三裂片圆形和三角形之分;均具三孔沟,外壁纹饰均为条纹孔穴型,具平行分叉条纹或平行条纹呈皱波状,脊表面有或少或多的孔穴.不同李品种在花粉粒大小、萌发孔、形态上均表现一定的差异性,对于李品种的区分、引种、选育和生产实践中具有一定的参考和应用价值.     

枸杞花蕾扫描电镜制样方法的探讨

以枸杞花蕾为材料,经常规石蜡切片及脱蜡技术,采用4种干燥法处理样品,再经电镀、扫描电镜观察拍照,通过图片分析比较,从而获得一种适合植物花蕾扫描电镜制样的干燥方法。结果表明:叔丁醇结合真空冷冻干燥仪干燥处理植物花蕾效果更佳,通过此法,获得的图像清晰,细胞及其组织的三维结构可被完好地保存     

蔬菜一年三作高效种植模式

为提高土地复种指数,增加单位面积经济效益,对郯城当地的蔬菜种植模式进行了搭配探索和分析,通过多年实践经验,筛选出了一套经济合理的栽培模式——苦瓜、马铃薯、香菜一年三作高效栽培。文章重点从模式配置、配套技术、栽培技术、效益分析等几方面对该模式进行了总结。     

An experimental study of hypercholesterolemia-induced disorder of Oddi's sphincter

AIM:To investigate the functional changes and histopathological basis of Oddi’s sphincter under hypercholesterolemia. METHODS:Twenty - four New Zealand rabbits were equally divided into be groups ran- domly. Experimental groups Ⅰ and Ⅱ fed with cholesterol - added forage for 4 and 8 weeks respectively before sacri- ficed. Images, functions and histopathological characteristics of Oddi’s sphincters of experimental groups were studied and compared with control group by cholangiography, catheter manometry, and quantitative analysis of nitric oxide synthase. RESULTS: The hasal pressures of proximal low - pressure segment of Oddi’s sphincter of hath experimen- tal groups Ⅰand Ⅱ (20.9±6.1 mmHg, 25 .6±9.1 mmHg, respectively) were higher than those of control group sig- nificantly (11.7±2.8 mmHg, P < 0.01, P < 0.01 ). The hasal pressures of distal high - pressure "valve" segment of both experimental pops (138 .4±45. 5 mmHg and 144. 5 ±40 .4 mmHg, respectively) were significantly higher than those of coned group (69.3±9.8 mmHg, P < 0.01 and P<0.01). The gallbladder volUmes of experimental group Ⅰ and Ⅱ (3.4±1.0 mL and 3.9±1.9 mL) were significantly larger than those of control group (2.0 ±1.9 mL, P < 0.05 and P < 0.01 ). Between groups there were no significant differences in nitric oxide synthase con- tents. Enlargement of the cell volume, wavy distortion of the cell membrane, loosening and disarrangement of myofila- ment, uneven in size and distribution of dense body, angulation of longitudinal axis between dense body and myofila- ment, swelling, congregated at one end of nucleoulus and disappearance of cristae of plasmosomes and concertration of chromosome at nucleolus margin were observed in experimental groups under electron-microscope. CONCLUSION: The diastole function of Oddi’s sphincter could be damaged by hyperecholesterolemia, inducing a functional disorder of Oddi’s sphincter with increased resistance in bile excretion. The result is retertion of bile in the gallbladder.     

甘肃省双孢蘑菇栽培的3种技术模式

甘肃省地处西北高原,地形复杂,气候类型多样,形成了双孢蘑菇栽培模式的多样化。从适宜区域、季节安排、栽培原料、品种选择、栽培设施、栽培特点及安全高效生产的栽培技术要点等方面,介绍了甘肃省双孢蘑菇栽培的3种技术模式,即河西走廊沿山冷凉区夏季反季节栽培技术模式、陇东南地区秋冬栽培技术模式和荒漠绿洲区越冬栽培技术模式。     

保水剂与供水方式对辣椒幼苗生长的影响

采用穴盘育苗,研究不同浓度下旱露植宝多功能保水剂与供水方式交互对辣椒幼苗生长的影响。结果表明:辣椒穴盘苗底部供水方式下灌水次数及灌水量均少于上部供水,可节约用水,最高节水率为36.98%;底部供水方式下保水剂浓度为4 g/L时对辣椒幼苗的生长量、形态指标及生理指标差异影响显著,可促进辣椒出苗,提高秧苗质量。     

新疆甜菜丸粒化单粒种高密高产高糖栽培模式

在新疆适宜种植甜菜的生态区,通过选择甜菜丸粒化单粒种,在高密度种植条件下,依据不同栽培管理阶段特点采用合理的管理措施,以实现甜菜单产97.5～105.0t/hm2、含糖率15%以上的目标,从而构建甜菜丸粒化单粒种高密高产高糖栽培模式。     

Effect of antioxidants TA9901 on the fibril formation of Aβ1-40 injected into cerebral cortex of rat brain

AIM: To clarify if TA9901, a natural antioxidants, could inhibit the formation of β-amyloid (Aβ) fibril when Aβ_1-40 were injected into cerebral cortex of rat brain, and explore the mechanism of action of TA9901 on Alzheimer disesse. METHODS: Twelve Wistar rats (250-300 g) were randomly divided into four groups (n=3). (1) control group; (2) TA9901 treatment group (ip 100 mg·kg^-1 ·d^-1); (3) Vitamin E (VE) treatment group (ip 100 mg·kg^-1 ·d^-1); (4) PBS group. 5 μL 0.2% Aβ_1-40 was immediately injected into the right side of the deep cerebral cortex of control, TA9901 and VE group rats. The animals were sacrificed at the seventh day after the injection. The sections of the rat brain that contained the injected field were examined with transmission electron microscopy and Congo red staining with polarized microscopy. RESULTS: Many depositions of high electron density were observed by electron microscopy in the field where Aβ_1-40was injected. They are intimately intermingled with macrophages and astrocytes. In the field, abou10nm fibrillar structures were observed that appeared similar to the fibrils seen in senile plaque (SP) of the brain of Alzheimer disease (AD). The fields in control and VE group contained richer Aβ fibrils than that in TA9901 group. After the sections stained with Congo red, A1-40aggregation demonstrated intense birefringence under, indication the formation of amyloid fibrils. In TA9901 group, there was a weak birefringence. CONCLUSIONS: TA9901 can inhibit the fibril formation of Aβ that was injected into deep cerebral cortex of rat brain, this indicates primarily that TA9901 may be a potential therapeutic drug to interfere with the progression of amyloidgenesis in AD.     

6种枇杷属植物花粉形态扫描电镜观察

应用扫描电镜,对1个栽培品种(早钟6号),5个野生类型(椭圆枇杷、大渡河枇杷、栎叶枇杷、台湾枇杷、台湾枇杷恒春变型)的花粉进行了形态观察。结果表明,6种枇杷属植物的花粉粒均为长球形,具3条萌发沟,萌发沟以等间距环状分布,属于N3P4C5型花粉。6种枇杷属植物的花粉外壁属于条纹-穴状纹饰类型,外壁均具条嵴,嵴洼处散生有覆盖层穿孔,但条嵴及穿孔的形态和分布差异明显。最后,结合花粉形态的演化规律,对大渡河枇杷的系统学位置及枇杷属植物的起源与进化进行了讨论。     

黄瓜绿斑驳花叶病毒传播方式的研究进展

黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)是葫芦科作物重要病毒,通过多种方式如种子、土壤、水、介体和机械接触传播,病害蔓延扩散迅速。该病害在许多国家和地区频繁报道,我国也曾大面积受到为害,尤其是西瓜嫁接地区,产业损失严重。带毒种子为初侵染源,发生过该病害的地块土壤也是重要的侵染源,发病植株可通过灌溉水以及机械接触传播病毒。为了更好地控制该病害的发生与流行,本文介绍了该病毒传播方式方面的研究进展。