Preliminary Research on Regional Material Flow Analysis： A Case Study of Chengyang District in Qingdao

The method ＂Material Flow Analysis （MFA）＂ is one of the effective tools to study law and quantification of material flow between economic system and ecological system. On the national level, economy-wide material flow analysis has been published for a number of countries. However, published studies on the regional or local level are still very limited and a standardized method does not exist yet. On the basis of framework of Material Flow Analysis proposed by Eurostat, the paper collected related data and analyzed material input and output for the Chengyang district in Qingdao. The results showed that DMI （Direct Material Input） and TMR （Total Material Requirement） in absolute number increased about 3.6 and 3.9 times, respectively from 1995 to 2004. Fossil fuel and mineral contributed to about 50.3%-76.3% of DMI. Imports of material increased about 5.0 times and became the most important Contributor to DMI, which showed that local economic growth was highly dependent on resources from other regions and countries. DPO （Domestic Processed Output） and TDO （Total Domestic Output） represent slow increasing trend, and DPO contributed to 22.2%-58.1% of TDO, suggesting local hidden flows were of obvious effect on TDO. The biggest component of DPO is CO2, approximately accounting for 90% of DPO. The material productivity increased 57.7% in last decade, reflecting improvement of efficiency of resources utilization in some extent. However, compared to developed countries and regions, material productivity of Chengyang district was relatively low. Therefore, in order to promote the sustainability, it is essential to develop circular economy and enhance materials productivity.     

杨树游离氨基酸组成和含量与青杨天牛危害的相关分析

为了研究不同品系杨树上青杨天牛(Saperda populnea)的危害与树木自然状态下树木体内营养性物质影响昆虫生长发育的游离氨基酸组成和含量之间的相关性,利用HPLC柱前AccQ.Tag衍生法,分析了迎春5号、1344、银中杨、小黑14和施×斯的韧皮部和木质部中游离氨基酸的组成及含量。在各杨树品系韧皮部中共检测出14种游离氨基酸:丙氨酸、精氨酸、天门冬氨酸、甘氨酸、组氨酸、异亮氨酸、亮氨酸、赖氨酸、蛋氨酸、苯丙氨酸、脯氨酸、苏氨酸、色氨酸、缬氨酸;木质部与韧皮部的游离氨基酸种类相近,但木质部缺少苏氨酸。各种杨树品系韧皮部中精氨酸、赖氨酸与被害率相关性较强;杨树木质部中精氨酸、组氨酸与被害率相关性较强。不同杨树品系的健康与被害的韧皮部和木质部在不同种游离氨基酸之间的差异性都很显著。     

猪圆环病毒2型山东分离株全基因组的克隆与序列分析

根据猪圆环病毒2型(PCV-2)全基因组序列,设计一对特异性引物,从疑似断奶仔猪多系统衰竭综合征(PMWS)病料中扩增出PCV-2基因组DNA,将基因片段克隆于pMD18-T载体,筛选获得含有相应片段的阳性重组质粒pMD18-T-PCV-2。对筛选出的阳性质粒进行测序。结果表明,PCV山东株的全基因组为1 767 bp,与国内外毒株核苷酸同源性高达99.6%。     

二十二份毛花猕猴桃种质资源果实品质的主成分分析与综合评价

为了更好地了解毛花猕猴桃种质资源的多样性,测定了浙江省丽水市境内22份毛花猕猴桃种质的8个品质指标,进行了变异分析、主成分分析、聚类分析和综合评价.结果表明,果实品质指标差异较大,单果质量的变异系数最大(38％);果实横径的变异系数最小(11％);聚类分析结果表明,22份毛花猕猴桃种质可以分为两大类,其中第一类种质果实...     

马尾松银松素合酶基因启动子区的克隆及特征分析

银松素合酶(pinosylvin synthase,PS)是合成银松素类植物抗毒素的关键酶。本文根据前期工作所获得的PS基因序列,设计基因特异引物,利用连接介导的染色体步行技术,从马尾松总基因组中扩增PS基因上游的启动子区;通过PLAN-CARE等分析软件对5′侧翼区近700 bp进行启动子特征分析,预测启动子区调控元件。结果表明,在翻译起始密码子上游167 nt处可能存在TATA-box。此外还发现3个GATA-box(-260 nt、-295 nt、-386 nt和-295 nt)和若干个TGAC-like序列。     

小兴安岭谷地云冷杉林土壤酶活性的异质性

利用传统统计学与地统计学相结合的方法对小兴安岭谷地不同死亡程度云冷杉林表层土壤（0~20cm）酶活性空间异质性和格局进行研究。结果表明：①土壤脲酶变异函数曲线的理论模型符合线性模型,土壤转化酶、碱性磷酸酶和过氧化氢酶的理论模型符合指数模型或球状模型。②土壤酶活性各项指标的空间变异主要是由结构性因素引起,土壤碱性磷酸酶活性和过氧化氢酶活性指标的空间自相关程度均属较强以上（空间结构比均在75%以上）,土壤转化酶为中等（空间结构比均在25%以上）,土壤脲酶在较弱到中等之间变化。③云冷杉林表层土壤过氧化氢酶的自相关范围最大（0.58~3.08 m）,转化酶和碱性磷酸酶分别为1.41~3.98 m和0.91~4.29 m,而土壤脲酶自相关范围最小（15.426~16.673 m）。④土壤转化酶、碱性磷酸酶和过氧化氢酶活性的空间格局明显,土壤转化酶、碱性磷酸酶、过氧化氢酶和土壤脲酶的分数维分别为1.930~1.990,1.910~1.986,1.923~1.997和1.936~1.970。     

Cloning and analysis of cross-intron genomic gene encoding ACO in Paeonia suffruticosa Andr.

The last step of ethylene biosynthesis in higher plants is to convert 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene by ACC oxidase (ACO). In our investigation, a cross-introns genomic DNA gene encoding ACC oxidase, PsgACO, was isolated from Paeonia suffruticosa. It revealed that PsgACO (FJ855434) has 1281 bases, containing four exons and three introns, encoding 312 amino acids. The four exons stretched from 1 to 105, 217 to 434, 592 to 925 and 1000 to 1281 bases. A splicing junction sequence of all the exon-introns conformed to the GT-AG rule in the cross-intron genomic DNA sequence of the ACO gene. PsgACO showed high homology to many characterized ACC oxidases both at the nucleic acid and amino acid levels. As well, twelve amino acid residues were conserved among many ACOs from other species. A phylogenetic tree analysis indicated that the amino acid of ACOs is quite conserved among the different eudicots. The phylogenetic tree showed that both the tree peony and herbaceous peony are quite isolated taxa. Bioinformatic analysis showed that the molecular weight of ACO is 35.29 kD, with a theoretical pI of 5.25. It is a non-secrete, stable hydrophilic protein, located in the cytoplasm.     

中国6种犬科动物直针毛的扫描电镜分析

应用扫描电镜对我国分布的6种犬科动物背部和腹部直针毛的鳞片花纹类型进行研究.结果表明,6种犬科动物直针毛鳞片花纹类型主要有杂波型、长瓣型、杂瓣型、扁平型和方瓣型,毛鳞片形态在属间和种间存在差异,同一种类身体不同部位毛的鳞片形态也有差异,同一根毛的不同部位也存在差异.依据本项研究,笔者探讨了利用犬科动物毛鳞片花纹进行物种识别的可行性及需要注意的问题.     

美国无架豇豆单株产量构成因素分析

[目的]明确影响单株产量的重要因素,为美国无架豇豆栽培以及良种繁育提供参考。[方法]定株调查每株美国无架豇豆的豆荚数、荚重、荚长、花序数,拉秧时测定主茎高、分枝数及主茎节数,并计算单株产量,进行单株产量构成因素的相关分析和通径分析。[结果]豆荚数对单株产量的影响最大,且主要是直接正效应;荚长对单株产量的直接正效应居第2位;花序数、分枝数、主茎高及主茎节数对单株产量的直接或间接影响都较小或微弱;所研究7个性状对单株产量的总贡献为0.938 1,说明没有漏掉影响较大的因素。[结论]生产中,从增加豆荚数着手来提高单株产量是直接而有效的,通过增大豆荚长度来提高单株产量也有一定效果。     

GIS空间分析功能在山地风景区规划中的应用

GIS地理信息系统具有强大的空间分析与数据管理能力,可应用于山地风景区规划的各个方面。试图运用GIS技术的空间分析功能对山地风景区进行坡度、坡向、高程、山体阴影度、可视性等方面的分析,提出山地风景区土地利用规划的方法。