RNAi及沉默通路调控研究进展

RNA干扰（RNA interference,RNAi）是由双链RNA（double-stranded RNA,dsRNA）介导的细胞内双链mRNA特异性降解的现象,属于转录后的基因沉默机制。迄今为止,RNAi已经是一种广泛应用于研究基因功能的最新和最有效的方法,并且在治疗人类和动物疾病中也有很大潜力。作者主要综述了RNAi的过程、小RNA的生物发生学及沉默通路的保护和调控。     

西宁盆地黄土区荷载条件下植被护坡力学效应

为探讨西宁盆地黄土区边坡在荷载条件下植物根系对边坡稳定性的影响,该项研究以西宁盆地西山长岭沟流域为试验区,在自建边坡上种植草本植物垂穗披碱草（Elymus nutans）和灌木植物柠条锦鸡儿（Caragana korshinskii）,通过在边坡坡顶施加载荷,边坡坡面布设位移计与土压力盒的方式,监测荷载条件下边坡坡面土体位移与土压力变化;在此基础上,对边坡植被根-土复合体开展剪切试验,分析荷载条件下边坡坡面不同位置处根-土复合体抗剪强度及其变化特征。试验结果表明：在坡顶荷载条件下,在边坡相同位置处的根-土复合体粘聚力c值均大于施加载荷前;3种植物边坡的根-土复合体粘聚力c值均大于相同条件的未种植素土边坡;在荷载条件下,组合植被边坡的坡面土体位移量显著小于单一植被边坡和素土边坡,组合植被边坡在竖直和水平方向的位移量与草本植被边坡相比减小1.60～1.77 mm,与灌木植被边坡相比减小3.78和4.11 mm,与素土边坡相比减小7.39和6.29 mm。研究结果对防治高寒半干旱地区坡面水土流失、浅层滑坡等地质灾害,具有参考价值和指导意义。     

FGF4在肺癌细胞体内和体外中表达差异的研究

FGF4已经被证明是癌基因,它涉及肿瘤的生长和转移,为了解FGF4的表达与肿瘤微环境的关系。我们利用FGF4抗体通过免疫组化对一名肺癌患者癌旁,癌组织,癌组织小鼠移植瘤,二次移植瘤以及原代培养的细胞爬片进行FGF4检测,探究其表达差异。通过对比癌组织与对照组（癌旁组织）,FGF4在癌巢中高表达;同样,移植瘤与二次移植瘤的癌巢中与癌旁组织比较,FGF4表达相对较高;但是将癌组织进行原代培养后,免疫组化检测细胞爬片FGF4,发现仅有5%±0.21%的肿瘤细胞表达FGF4,对照蛋白Cytokine作为肿瘤标记物,则在100%的肿瘤细胞中表达。研究提示免疫组化检测到FGF4在体内和体外表达不同,提示肿瘤细胞FGF4的表达与肿瘤微环境调控密切相关,肿瘤微环境对肿瘤细胞的FGF4的调控有着重要作用。     

CD8+T细胞在不同年龄家兔胃肠道的分布规律

探讨不同年龄组家兔胃肠道各段CD8⁺T细胞的分布特征,为进一步研究CD8⁺T细胞的作用奠定形态学基础。采用免疫组织化学技术对不同年龄组(幼年组、青年组、老年组)家兔胃肠道内CD8⁺T细胞进行染色、观察及统计分析。结果显示,家兔的CD8⁺T细胞多呈圆形、椭圆形或呈不规则形。在不同年龄组家兔,胃肠不同部位的分布有如下特点:家兔的CD8⁺T细胞主要分布于胃肠道的固有层中;青年组CD8⁺T细胞的分布数量显著高于幼年组和老年组;各肠段之间CD8⁺T细胞分布的数量差异不显著,但其分布的总量按照小肠、大肠、胃的顺序依次降低。研究结果显示,CD8⁺T细胞在家兔小肠的分布最多,并且其数量随着年龄的增长呈现先上升后下降的趋势,青年家兔胃肠的分布最为丰富。     

Gross anatomy,computed tomography,magnetic resonance imaging and bone mineral densitometry of the ovine metacarpo/metatarsophalangeal joint

This study was aimed to provide anatomical atlas of the ovine metacarpo/metatarsophalangeal joints using computed tomography (CT) scan and magnetic resonance imaging (MRI), as well as to investigate bone mineral density (BMD) and morphometric features of this joint. The limbs of twenty adult Sanjabi sheep were examined. Imaging was performed using a 16-slice multi-detector CT scanner and a 1.5 Tesla MRI scanner. The obtained images were correlated with corresponding anatomical sections. BMD was measured by Dual Energy X-ray Absorptiometry (DEXA) method. Also morphometric features included distance between metacarpal and metatarsal heads, width of metacarpal and metatarsal head, length, width and the height of the proximal sesamoid bones were measured. In MRI images, compact bones, ligaments and tendons showed less signal intensity (hypointense). The fatty tissue and bone marrow had more signal intensity (hyperintense), but articular cartilage and synovial fluid showed moderate signal intensity. BMD in the left hind limb was significantly higher than other three limbs (p ≤ 0.05). The length of proximal sesamoid bones in left forelimb was higher than right forelimb (p ≤ 0.05). The thickness of proximal sesamoid bones in left limbs was higher than those in their counterparts (p ≤ 0.05). The width of the medial head in the forelimbs was significantly greater than the hind limbs (p ≤ 0.05). The present results might be useful in managing the clinical techniques on this joint. Larger volume of the proximal sesamoid bones and wider medial head in the forelimbs compared to the hindlimbs impel this speculation that the centre of gravity is closer to the forelimbs.     

乌江流域茶园土壤理化性状对茶叶品质影响的研究

对乌江流域主要茶场茶园土壤理化性状、茶叶生化成份测定结果表明：不同母岩发育的土壤理化性状各不相同，其对应的茶叶品质也不相同。土壤理化性状及茶叶品质优劣依序为硅质黄壤＞砂页岩黄壤＞第四纪粘质黄壤＞小黄泥＞黄棕壤     

贫困地区农业技术推广新模式：发展小区域农业技术推广户 ——基于甘肃省部分贫困县的调查思考

（1甘肃农业大学经济管理学院，兰州 730070；2兰州资源环境职业技术学院，兰州 730020）     

A Theoretical Analysis of Underpricing in IPOs Based on the Irrational Behavior of Investors

Employing the standpoint of irrational investors in the behavior finance, the authors study the relation on the irrational behavior of medium and small Investors and underpricing of IPO in our country. The paper premises that a revenue-maximizing issuer will be regarded as the target function, and the rational institutional investors can be delegated the task of holding the stocks in the after-market for resale to the irrational medium and small investors--thereby extracting surplus from them, finally the issuer can maximize their expected revenue. We model the process mentioned above and find that the high underpricing of IPO in our country has the direct relation with numerous the irrational medium and small investors existing in our country who cause the underpricing.