基于颠茄发根的外源基因表达系统的建立

 用携带植物高效表达载体pCAMBIA1304 ⁺ “解除武装”的重组C58C1工程菌转化颠茄无菌苗真叶, 发根诱导率达100%。PCR检测证实发根型质粒pRiA4和表达型质粒pCAMBIA1304 ⁺均能整合到颠茄发根基因组内, 共转化率达30%。建立了基于颠茄发根的高效外源基因表达系统, 为将托品烷类生物碱东莨菪碱的生物合成关键酶基因导入颠茄, 实现其次生代谢工程及开展分子育种奠定了基础。     

基于Hydrus-1D模型的冬小麦根系层水分渗漏分析

利用Hydrus-1D模型模拟了河北省石津灌区冬小麦水分渗漏情况。结果表明,研究区40％的灌溉降雨量和38％的总供水量流出了200cm边界层,通过设置12种方案得到初始饱和度、2次春灌水量是影响灌区渗漏的主要因素,而二水时间间隔和第一水灌水日期影响相对较小。通过设置426种方案,分别得到了不同初始饱和度时,2次春灌灌水...     

Root exudation of prometryn and its metabolites from Chinese celery (Apium graveolens)

Root exudates from Chinese celery (Apium graveolens) and Chinese cabbage (pak choi, Brassica chinensis) plants treated by prometryn, an herbicide, were qualitatively and quantitatively investigated and compared under hydroponic cultivation. Prometryn and its metabolites released into the nutrient solution were analyzed by ultra-performance liquid chromatograph coupled with orbitrap mass spectrometer to investigate whether this xylem-mobile herbicide is exuded from the roots. The results showed that celery and pak choi had different root exudation profiles. Celery metabolized prometryn to prometryn sulfoxide and released both compounds from the roots. In contrast, pak choi barely metabolized or actively released prometryn from the roots. The concentration of prometryn sulfoxide released from celery after 96 hr was 21 µg/L, which was nearly one-third that of released prometryn. Our results indicate that the root exudation and translocation of xylem-mobile herbicides could be significant in plants and are highly species dependent compared with phloem-mobile herbicides.     

Drought responses of above‐ground and below‐ground characteristics in warm‐season turfgrass

Water shortages have become more chronic as periodic droughts prolong and water demand for urban and agricultural use increases. Plant drought responses involve coordinated mechanisms in both above‐ and below‐ground systems, yet most studies lack comparisons of root and canopy responses under water scarcity and recovery. This is particularly true of research focused on warm‐season turfgrasses in sandy soils with extremely low water holding capacity. To address the lack of examination of coordinated stress and recovery responses, this study compared the above‐ and below‐ground plant responses during a dry‐down period of 21 days and recovery among four warm‐season turfgrass species in the field. Canopy drought responses and recovery were quantified using digital image analysis. In situ root images were captured using a minirhizotron camera system. Common bermudagrass [Cynodon dactylon (L.) Pers.] endured the entire drought period without losing 50% green cover while other species lost 50% green cover in 11–34 days predicted from the regression. The interspecific differences in drought resistance were mainly due to root characteristics. Other drought mechanisms appear to be responsible for differences identified in drought resistance between “Zeon” and “Taccoa Green” manilagrass [Zoysia matrella (L.) Merr.]. Recovery was delayed for up to 2 weeks in the second year, warranting further evaluation for turfgrass persistence under long‐term drought. Three‐week drought posed no threat to the survival of zoysiagrass. Species and genotypic variations were found in achieving full post‐recovery, which can be used to develop water conservation strategies and to adjust consumer expectations.     

小麦上三叶配置比例对产量性状的影响

选用不同株型、叶型的小麦品种 (系 ) 1 0个 ,研究了上三叶配置比例特点及与产量性状的相关、遗传参数和选择效果。结果表明 :不同类型品种 (系 )的上三叶配置比例各不相同 ,旗叶、倒二叶、倒三叶的长宽比值与产量呈正相关 ,旗叶与倒二叶、倒三叶的长度之比、宽度之比、面积之比与产量呈负相关 ,探讨了小麦上三叶配置比例在株型育种和高产育种中的应用     

落叶松播种苗使用钼酸铵根外追肥试验报告

通过对落叶松播种苗根外追施钼酸铵液，促进苗高，地径生长，较明显地提高了苗木整体质量，对苗木木质化和提高苗木对不良环境的抵抗力有着积极的促进作用。     

Genetic Variability in Phosphorus Acquisition and Utilization Efficiency from Sparingly Soluble P-Sources by Brassica Cultivars under P-Stress Environment

Plants display an array of classical strategies to maximize phosphate (Pi) acquisition from sparingly soluble P sources. Acclimation to Pi-stress via elegant Pi-starvation induced (PSI) adjustments would reduce our current overreliance on expensive, polluting and non-renewable Pi-fertilizers. Nevertheless, differences in the ability of various species to solubilize sparingly soluble P-sources have been often evidenced; inter-cultivar variations are scarcely documented. Brassica is known as an effective, non-mycorrhizal user of sparingly soluble P-sources. Various growth parameters and biomass accumulation by genetically diverse Brassica cultivars were determined in four experiments using hydroponics and quartz sand culture media. Role of PSI root mediated pH changes, organic anions (OAs) exudation and altered root architecture in mobilization and acquisition of sparingly soluble P-forms [Jordan rock-P (RP) at 2 g l⁻¹ and Ca₃(PO₄)₂ (TCP) at 0.2 g l⁻¹ respectively] was investigated. Cultivars showed considerable genetic variations in biomass accumulation, various growth parameters and root–shoot ratio. Concentration and total uptake of P, specific absorption rate of P, P-transport rate and P-utilization efficiency (PUE) were also significantly (P < 0.001) different for various cultivars and their dry matter was significantly correlated with P-uptake [r = 0.94** (significant at 1% level)]. P-tolerant cultivars showed substantial decrease in solution media pH because of H⁺ efflux and exuded more carboxylates than low P-sensitive cultivars under P-starvation. P-uptake by cultivars increased linearly with decreasing pH. The amount and types of OAs exuded from the roots of P-starved plants differed from those of plants grown under P-sufficient environment. In split pot study, with TCP and RP supplied spatially separated from other nutrients, efficient cultivars were still able to mobilize RP and TCP more efficiently than inefficient cultivars. In rhizobox study, the elongation rates of primary roots decreased but the elongation rates of the branched zones of primary roots and the length of lateral roots increased under P-starvation. Tested cultivars showed genetic diversity in accessing, mobilization, acquisition and utilization of Pi from sparingly soluble P forms. An arrange marriage of plant traits can explain cultivar’s access to different forms of sparingly soluble P, and in addition to altered lateral root topology and enhanced P-uptake and PUE, enhanced H⁺ efflux and OAs exudation are key factors in Pi scavenging from extra cellular sparingly soluble P-forms.     

临安市竹笋加工业持续发展对策初探

分析了临安市发展竹笋加工业的优势及存在的问题，提出促进其持续发展的对策。     

混交林纯林中毛竹出笋退笋特性研究

应用出笋量的逻辑斯谛 ( L ogistic)模型研究了出笋量随时间增长的变化规律 ,结果表明 :出笋前期 (前 1 0 d)出笋数占总数的 1 5%左右 ,出笋中期 (第 1 0～ 2 0天 )占 75%,出笋末期 (第2 0天以后 )占 1 0 %;对退笋特征的观察及其规律的研究得出 :出笋前后期退笋数量少但退笋率高 ,而出笋中期退笋数量多但退笋率低 .小于 1 0 cm的浅鞭竹笋多属退化笋 .纯林中的出笋比混交林中的出笋持续天数短 ,退笋率高 ,为此混交林更有利于竹笋的生长 .出笋退笋规律可用来指导生产 ,为留养和挖取竹笋数量提供依据     

地膜覆盖下豆禾混播草地根系的动态研究

使用差额法连续分层次地对燕麦＋豌豆人工草地的根系在地膜覆盖条件下的生长发育动态进行了研究，结果表明：燕麦根系的58％分布浅，豌豆的77％根系分布在10～20cm深，两者混播后，其根系可有效地互补，且在地膜覆盖条件下互补性增强。同时地膜覆盖后，使根系的分布更浅，根量减少。