猪场废水的生物处理技术及其效果

规模化养猪场所产生的废水污染，已成我国农村面临污染的重要来源。针对这种污染，近年来出现了多种养猪场废水处理法，其中研究和应用最广的是生物处理法。本文通过对目前国内猪场废水生物处理法的现状分析后认为：用生物处理法处理猪场废水是今后猪场废水处理的发展方向；而生物处理法自身的发展方向是混合处理法；计算机和生物工程技术将进一步与生物处理法相结合运用于猪场废水处理。     

马传贫驴白细胞弱毒疫苗株跨膜蛋白主要免疫决定区基因的克隆与表达

从感染驴白细胞的马传贫驴白细胞弱毒疫苗株前病毒DNA中克隆了编码跨膜蛋白主要免疫决定区(TMIR)的基因，并在大肠杆菌中进行了表达。所表达的融合蛋白有一部分是可溶的，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。在间接酶联免疫吸附试验(ELISA)和免疫印迹试验中，重组的TMIR蛋白可与马传贫阳性血清样品发生反应，而与健康马血清无任何反应。这表明该重组蛋白具有良好的抗原性和特异性，可用于马传贫弱毒疫苗株在体内外复制、接种马体内免疫应答及马传贫诊断的研究。     

Validation of the accuracy of household reporting of subsistence fishing catch and effort: a Fijian case study

Abstract  The accuracy of household reporting of subsistence fishing catch and effort and seafood consumption on the Fijian island of Ono-i-Lau was studied. A creel survey was carried out concurrently to validate the household survey data. Reported estimates of fishing participation, effort, and fish consumption were not significantly different to the creel survey estimates. Householder's appeared to overestimate the number of fish from the most abundant family Lethrinidae, and underestimate rarer fish. However, there was no significant difference between the reported and observed contribution of the majority of finfish families and invertebrate taxa. The reported and observed catch rate estimates were not significantly different, indicating that Ono-i-Lau villagers are relatively accurate in their estimation of the number and size of fish from recalled catches.     

Growth of triploid oyster, Crassostrea madrasensis (Preston)

The performance of I and II meiotic triploids and control oysters (Crassostrea madrasensis) reared at Tuticorin Bay was compared to determine if the improvements in the growth of edible oysters were additive to faster growth in triploids. After a grow‐out period of 12 months, both mean whole weights and shell heights were in order I meiotic triploid>II meiotic triploid>control. Mean whole weights and shell height of different oyster lines were all significantly different (P<0.05). On an average, larger morphological traits indicated that growth improvements from triploids were additive, and throughout the study triploid oysters maintained faster growth rate than their diploid siblings. Condition index and adductor muscle diameter of both triploids were higher than those of control.     

人工饲养条件下荒漠猫的饲养与繁殖管理

在人工饲养条件下,通过模拟野外栖息环境,加强科学饲养管理,成功繁殖荒漠猫的饲养管理方法和荒漠猫的发情、交配、妊娠、哺乳等繁殖行为。同时对在西宁动物园产下并成活的3只幼猫的体尺、体重、形态和行为的变化进行了描述。     

水稻香亭病(Balansia oryzae-sativae Hashioka)--一种影响杂交水稻种子出口的病害

水稻香亭病（Balansia oryzae-sativae Hashioka）,又被称为水稻＂一柱香＂病（Ephelis oryzae Sydow）,是当前影响杂交水稻种子出口的一种真菌病害.从该病原菌的命名、分布、寄主、生物学特性、危害症状、经济损失、检测方法和防治等方面进行了概述.     

猪带绦虫六钩蚴cDNA文库的构建与筛选

在体外将猪带绦虫虫卵孵化为有活力的六钩蚴。采用商品化试剂盒抽提六钩蚴总 RNA、m RNA,反转录为双链c DNA,再加 Eco R Adapter。在去除小片段后 ,dsc DNA与 λgt11噬菌体 DNA连接 ,经蛋白包装 ,构建了猪带绦虫六钩蚴λgt11c DNA文库。该文库效价为 3.5× 10 9pfu/ m L ,重组 DNA片段大小为 0 .5～ 3.2 kb,平均为 1.6 kb,蓝白斑比 1∶ 9。用抗体探针对文库进行免疫学筛选 ,在消除非特异性反应的基础上筛选约 10 6 重组子 ,共得到 118株强阳性克隆 ;应用 PCR鉴定上述部分阳性克隆 ,均扩增到 0 .5 kb以上的片段。结果显示 ,构建的文库合格 ,含六钩蚴所有抗原基因 ,可用于六钩蚴 c DNA克隆的筛选 ;用免疫学与 PCR联合筛选 c DNA文库 ,可消除假阳性     

酸碱对低活力苜蓿种子萌发及初期生长的影响

以H2SO4，HCl，HNO3及NaOH为激活荆，研究其对低活力苜蓿种子萌发及初期生长产生的影响。结果表明：不同酸碱处理及其时间不同，效果差异明显，但各处理在不同程度上均对苜蓿种子萌发和初期生长有促进作用；尤其是短时间HNO3处理，对激发苜蓿种子活力效果最佳。     

加入WTO后新疆棉花生产可持续发展探讨

我国加入WTO后，新疆棉花生产面临着一系列的挑战与机遇。在阐述新疆棉花生产的优势、劣势及生产现状基础上，剖析了新疆棉花的市场竞争力和我国加入WTO后对新疆棉花生产的正面和负面影响，最后提出了新疆棉花生产可持续发展的对策建议。