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M. Sandholm A. Vidovic A. Puotunen-Reinert S. Sankari K. Nyholm H. Rita 《Acta veterinaria Scandinavica》1995,36(2):255
The discriminating ability of 15 parameters alone or in combinations, including results from analysis of plasma endotoxin, the Nycomed plasma D-Dimer test and phospholipase A2, were analyzed to predict morbidity and mortality in equine gastrointestinal colic. Endotoxaemia was a characteristic feature of the colic horses. The problem of adequately predicting non-survivors among colic horses required several parameters to be included in the logistic model: if the “classical parameters”, (heart rate, respiratory rate, PCV, anion gap) were included in the model, addition of plasma D-dimer, phospholipase A2, and Cl- significantly improved the predictive value of the logistic model. Increasing heart rate and D-dimer together with decreasing chloride was a risk factor for nonsurvival. The sensitivity of this three-parameter logistic model to predict nonsurvival was 78% and specificity 77%. The Nycomed D-Dimer test is recommended as a horse-site test to predict disseminated intravascular coagulation and nonsurvival in equine colic. 相似文献
13.
小鹅瘟PCR诊断方法的建立和初步应用 总被引:11,自引:0,他引:11
根据发表的鹅细小病毒B株VP3基因序列 ,设计合成了 1对寡聚核苷酸引物。以国内分离的小鹅瘟病毒为摸板 ,筛选了 (PCR)最佳反应条件 ,并进行了敏感性、特异性和初步应用试验。结果表明 ,在 4 0 μL体积中 ,PCR最佳系统组成为 2uTaq酶、0 5mol/LdNTP和 2 0 pmol引物 ;最佳反应参数为 96℃变性 4 0s ,5 4℃退火 1min ,72℃延伸 1min ,30个循环。本方法可检出 2 5× 10 -1 5EID50 小鹅瘟病毒 ,并且仅能从小鹅瘟病毒的鹅胚培养物中扩增出与设计值大小相同的 375bp核苷酸片断 ,对照病毒扩增结果为阴性。通过对 6份临床病料的检查 ,2份呈阳性 ,其中 1份来自有典型小鹅瘟症状的雏鹅 ,1份来自无典型小鹅瘟症状的雏鹅。由此说明 ,本试验所建立的诊断小鹅瘟的PCR方法具有潜在的临床应用价值 相似文献
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利用卫星遥感技术进行宏观监测是我国荒漠化监测的重要手段,它具有高效经济的特点。本文阐述了中荷合作项目中荒漠化能量与水平衡监测系统的技术方法及主要应用。 相似文献
16.
Meadows RL MacWilliams PS 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1994,23(2):54-62
Varying terms and criteria have been used in the veterinary literature to characterize milky opaque pleural effusions through the years. This article addresses ideas widely repeated in the veterinary and human literature upon which time, experience, diagnostic techniques, experimental data, and improved understanding of pathogenesis have cast doubt. Topics discussed include terminology, pathogenesis of chylous and pseudochylous effusions, criteria for differentiation of chylous from pseudochylous effusions, and clinicopathologic changes associated with drainage of chylous effusions. 相似文献
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本文利用 RS、GIS的高新科技手段 ,对乌拉盖综合开发区土地资源质量等级划分与评价进行了认真研究 ,编制出乌拉盖综合开发区土地资源质量等级分布图 ,对各不同土地资源质量等级进行了评价 ,为该地区确定最佳土地利用方向、提高土地资源的生产效益、经济效益和生态效益提供科学依据 相似文献
19.
AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on. 相似文献
20.
Detection of the Defoliating Pathotype of Verticillium dahliae in Infected Olive Plants by Nested PCR 总被引:2,自引:0,他引:2
Jesús Mercado-Blanco Dolores Rodríguez-Jurado Encarnación Pérez-Artés Rafael M. Jiménez-Díaz 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(1):1-13
Spread of Verticillium wilt into newly established olive orchards in Andalucía, southern Spain, has caused concern in the olive industry in the region. This spread may result from use of Verticillium dahliae-infected planting material, which can extend distribution of the highly virulent, defoliating (D) pathotype of V. dahliae to new areas. In this study, a molecular diagnostic method for the early in planta detection of D V. dahliae was developed, aimed especially at nursery-produced olive plants. For this purpose, new primers for nested PCR were designed by sequencing a 992-bp RAPD marker of the D pathotype. The use of the specific primers and different nested-PCR protocols allowed the detection of V. dahliae pathotype D DNA in infected root and stem tissues of young olive plants. Detection of the pathogen was effective from the very earliest moments following inoculation of olive plants with a V. dahliae pathotype D conidia suspension as well as in inoculated, though symptomless, plants. 相似文献