The combined effect of drought and light on different physiological and biochemical traits was assessed in cork oak (Quercus suber L.) seedlings grown under two levels of light availability and submitted to a long-standing drought. Watering was withdrawn after germination and seedlings were allowed to dry to a water content of ca. 50% of field capacity. At this point, water-stressed seedlings were grown under moderate drought and two light regimes: high light (HL—50%) and low light (LL—2%). Soil water in control plants was kept close to field capacity (90–100%) for both light environments. Water-relations parameters derived from P–V curves, gas exchange and water status at predawn (Ψpd) were evaluated at twice during the experiment. Nitrogen and chlorophyll contents were determined in the same leaves used for the gas exchange measurements. In addition, maximum rate of carboxylation (Vcmax) and electronic transport (Jmax) were derived from A–Ci curves in well-watered seedlings.
The variation on moisture availability during the experiment was the same under both light environments. In control plants, Ψpd was over −0.3 MPa at the two harvests, while stressed seedlings decreased to −0.9 MPa, with no differences between light treatments. Water stress decreased osmotic potentials at full (Ψπ100) and zero turgor (Ψπ0). The regressions between both potentials and Ψpd showed a higher intercept in shade grown seedlings. This fact will point out the higher osmoregulation capacity in sun seedlings whatever water availability.
Nitrogen investment on a per leaf mass (Nmass), chlorophyll content (Chlmass) and SLA tended to show a typical pattern of sun-shade acclimation. Thus, the three parameters increased with shade. Only for Nmass there was a significant effect of watering, since water stress increased Nmass.
LL plants showed a lower photosynthetic capacity in terms of maximum net photosynthesis at saturating light (Amax), which was related to a decrease in Vcmax and Jmax. Both parameters varied with specific leaf area (SLA) in a similar way. The low-light environment brought about a higher nitrogen investment in chlorophyll, while under high-light environment the investment was higher in carboxylation (Vcmax) and electronic transport (Fmax).
Stomatal conductance to water vapour (gwv) and Amax were lower in low-light seedlings independently of watering. In addition, there was a trend to keep higher intrinsic water use efficiency (IWUE) under high light environment. The increase of IWUE under water stress was higher in HL seedlings. This was as consequence of the steeper decline in gwv as Ψpd decreased. The decrease of Amax with Ψpd occurred in a similar way in LL and HL seedlings. Thus, the HL seedlings tended to sustain a higher ability to increase IWUE than LL seedlings when they were submitted to the same water stress. 相似文献
Spiraea pubescens, a common shrub in the warm-temperate deciduous forest zone which is distributed in the Dongling Mountain area of Beijing,
was exposed to ambient and enhanced ultraviolet-B (UV-B, 280–320 nm) radiation by artificially supplying a daily dose of 9.4
kJ/m2 for three growing seasons, a level that simulated a 17% depletion in stratospheric ozone. The objective of this study was
to explore the effects of long-term UV-B enhancement on stomatal conductance, leaf tissue δ13C, leaf water content, and leaf area. Particular attention was paid to the effects of UV-B radiation on water use efficiency
(WUE) and leaf total nitrogen content. Enhanced UV-B radiation significantly reduced leaf area (50.1%) but increased leaf
total nitrogen content (102%). These changes were associated with a decrease in stomatal conductance (16.1%) and intercellular
CO2 concentration/ air CO2 concentration (Ci/Ca) (4.0%), and an increase in leaf tissue δ13C (20.5‰), leaf water content (3.1%), specific leaf weight (SLW) (5.2%) and WUE (4.1%). The effects of UV-B on the plant were
greatly affected by the water content of the deep soil (30–40 cm). During the dry season, differences in the stomatal conductance,
δ13C, and WUE between the control and UV-B treated shrubs were very small; whereas, differences became much greater when soil
water stress disappeared. Furthermore, the effects of UV-B became much less significant as the treatment period progressed
over the three growing seasons. Correlation analysis showed that enhanced UV-B radiation decreased the strength of the correlation
between soil water content and leaf water content, δ13C, Ci/Ca, stomatal conductance, with the exception of WUE that had a significant correlation coefficient with soil water content.
These results suggest that WUE would become more sensitive to soil water variation due to UV-B radiation. Based on this experiment,
it was found that enhanced UV-B radiation had much more significant effects on morphological traits and growth of S. pubescens than hydro-physiological characteristics.
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Translated from Journal of Plant Ecology, 2006, 30(1): 47–56 [译自: 植物生态学报] 相似文献
The nitrogen (N) cycling was elucidated in a 40-year-old subtropical evergreen broad-leaved forest dominated by Cyclobalanopsis glauca growing on red soil in Zhejiang Province, East China. The concentrations of N in the representative species ranged from 0.49%
to 1.64%, the order of which in various layers was liana and herb layers > understory layer > tree and subtree layers; in
various organs was leaf > branch > root > trunk; and aboveground parts > underground parts. The sequence of the concentrations
of N in C. glauca was understory > tree > subtree layer; young and high-growing > old organs; reproductive > vegetative organs. Seasonal dynamics
of the concentrations of N in C. glauca in the tree and subtree layers was comparatively stable. It was lower in autumn (October) in root, branch, and leaf in the
tree layer, and low in January in the understory. There was no evident change in regularity of the concentrations of N in
varying diameter classes. The concentrations of N in the litterfall, precipitation, throughfall, litter layer, and soil were
0.74%–2.30%, 0.000,038%, 0.000,09%, 1.94%, and 0.59%, respectively. The standing crop of N in the plant community was 1,025.28
kg/hm2, accumulation in the litter layer was 224.88 kg/hm2, and reserve in the soil was 55,151 kg/hm2. Annual retention of N was 119.47 kg/hm2, return was about 84.13 kg/hm2, among which litterfall was 78.49 kg/hm2 and throughfall, 5.64 kg/hm2. Annual absorption of N was 203.60 kg/hm2. Annual input of N through incident precipitation was 4.88 kg/hm2. Compared with other forest types, cycling rate of N in the community was lower than in deciduous broad-leaved forests, rain
forests, and mangroves, and was moderate in evergreen broad-leaved forests. N use efficiency of this forest was moderate among
the forest types cited. According to the characteristics of the biocycle of phosphorous, it was concluded that N availability
in the soil of this forest was not lower, and phosphorous not N was the limiting factor in the growth of plants in this community.
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Translated from Acta Ecologica Sinica, 2005, 25(4): 740–748 [译自: 生态学报, 2005, 25(4): 740–748] 相似文献
Sesbania [Sesbania sesban (L.) Merr.] fallows are being promoted as a means for replenishing soil fertility in N-depleted soils of small-scale, resource-poor
farmers in southern Africa. Knowledge of soil water distribution in the soil profile and water balance under proposed systems
is important for knowing the long-term implications of the systems at plot, field and watershed levels. Soil water balance
was quantified for maize (Zea mays L.) following 2-year sesbania fallow and in continuous maize with and without fertilizer during 1998–1999 and 1999–2000 at
Chipata in eastern Zambia. Sesbania fallow increased grain yield and dry matter production of subsequent maize per unit amount
of water used. Average maize grain yields following sesbania fallow, and in continuous maize with and without fertilizer were
3, 6 and 1 Mg ha−1 with corresponding water use efficiencies of 4.3, 8.8 and 1.7 kg mm−1 ha−1, respectively. Sesbania fallow increased the soil-water storage in the soil profile and drainage below the maximum crop root
zone compared with the conventionally tilled non-fertilized maize. However, sesbania fallow did not significantly affect the
seasonal crop water use, mainly because rainfall during both the years of the study was above the normal seasonal water requirements
of maize (400 to 600 mm). Besides improving grain yields of maize in rotation, sesbania fallows have the potential to recharge
the subsoil water through increased subsurface drainage and increase nitrate leaching below the crop root zone in excess rainfall
seasons.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
Poultry nutritionists should constantly evaluate their feeds and feeding programs in view of the changes that continue to be made to the potential growth rate of broilers and egg production in laying hens with the objective of providing the birds with the optimum economic level of nutrients at all times. In this review, the models generated at Universidade Estadual Paulista (UNESP) Jaboticabal, to estimate the optimum intake of amino acids (AA) for broilers and laying hens are presented. These models were used to estimate the variation in AA intake within a broiler population making use of coefficients of variation that represent the real situation in a commercial broiler operation. The range of AA intakes obtained was compared to some commonly applied feeding programs and recommendations. Based on results of our developed models and other feeding programs, feeds were formulated and growth was predicted using a growth simulation model (Avinesp). The prediction of growth showed that these models are reliable and useful tools to assist the nutritionists in decision making. In a study with laying hens, the Reading model (RM) was used to establish optimum economic AA intakes in a flock of hens. The input variables were the means and standard deviations of egg output and BW, and the relationship between the marginal cost of the AA and the marginal revenue for eggs. Based on this model, the optimum intake of each AA can vary with each of the variables considered. The models proposed in our study enable important decisions to be made regarding AA intakes to be used to obtain optimum economic performance from a population of broilers and laying hens. 相似文献