电阻抗法测定杨树无性系抗寒性

以7个杨树无性系当年生枝条为材料,应用电阻抗法对其抗寒性进行了测定,对比分析了弛豫时间（τ） 、弛豫时间分布系数（ψ）、电阻率（r）、电阻率（r₁）、胞外电阻率(r_e)、胞内电阻率(r_i)拟合的半致死温度（LT₅₀）,并与电导率法进行了对比。结果表明:r_e是相对适用的一个参数,可较好反映出不同无性系的抗寒能力;采用电阻抗法测定不同无性系抗寒性结果与传统的电导率法的结果基本一致,具操作更简单、快捷等优越性。     

Distinct strains of the re‐emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina

Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT‐PCR and sequencing. In this work, the virus has been successfully purified and a double‐antibody sandwich (DAS‐) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co‐infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.     

温针灸治疗中风后尿潞留患者的临床观察

〔摘要〕目的观察温针灸在中风后尿潞留患者中的治疗作用、方法将61例中风后尿潞留留置导尿患者随机 分为治疗组33例,对照组28例,在进行基础治疗情况卜,治疗组采用温针灸治疗,对照组采用一般治疗,1次//d ,4 次为1个疗程,隔2 d开始卜1疗程,共治疗4个疗程,未满4个疗程己治愈者,停i1=治疗并统计治疗结果〔结果 温针灸组总有效率为84.90Ic,对照组为64.30Ic,温针组疗效优于对照组(P<0.05 );两组治疗后有效病例起效时间比 较,温针组优于对照组(P<0.05 )〔结论温针灸治疗中风后尿潞留有一定的临床意义,值得采用、     

影响椰心叶甲啮小蜂寄主接受行为因子的探讨

对椰心叶甲啮小蜂的复眼和触角分别用水溶性黑色素和液体石蜡处理以探讨其视觉、嗅觉在其寄主接受行为过程中的作用;分别用椰子心叶、A4白纸、黑色薄膜3种不同方式包裹椰心叶甲蛹以探讨小蜂视觉在其寄主接受行为过程中的作用;把椰心叶甲蛹在-81℃超低温冰箱下冰冻10min后以探讨寄主蛹动对椰心叶甲啮小蜂寄生行为的影响。结果表明,椰心叶甲啮小蜂寻找寄主时嗅觉起主要作用,视觉仅起辅助作用,而寄主蛹动对该蜂接受寄主行为的影响不大。     

危害核桃的小蠹种类及防治对策

简述了辽宁省核桃栽培区危害核桃的黄须球小蠹，小咪小蠹，核桃咪小蠹，小粒材小蠹等的形态特征，生物学特性及防治对策。     

Desorption of myo‐inositol hexakisphosphate and phosphate from goethite by different reagents

Inositol phosphates are abundant organic phosphates found widely in the environment. The sorption and desorption of organic phosphate (P_o) are important processes in controlling the mobility, bioavailability and fate of phosphorus (P) in soil and sediment. The desorption characteristics of myo‐inositol hexakisphosphate (IHP) and inorganic phosphate (P_i) from goethite were studied by pre‐sorption of IHP or P_i followed by desorption by KCl, H₂O, and citrate. Batch experiments and in situ attenuated total reflectance Fourier transform infrared (ATR‐FTIR) spectroscopy were used to investigate the desorption of IHP/P_i. The desorption percentage of IHP/P_i by citrate was much higher than that by H₂O/KCl. The desorption of P by citrate was mainly achieved through ligand exchange, and the desorption increased with decreasing pH. Desorption by H₂O was slightly greater than that by 0.02 M KCl because the electrostatic repulsion between the P molecules is larger in H₂O. Due to the higher affinity of IHP for goethite than that of P_i, the maximum desorption of IHP was lower than that of P_i. Desorption curves (desorption concentration in solution vs. sorption density) of IHP or P_i on goethite by KCl or H₂O was well fitted by an exponential equation, while those by citrate were well fitted by a linear equation. The desorption amounts of P in the first cycle account for more than 58% of the total desorption followed by substantial decreases in the second and third cycles. There was a re‐sorption of P_i from solution in the late stage of desorption by KCl and H₂O, resulting in a sharp decrease in desorption. Re‐sorption of IHP did not occur, which is probably due to its poor diffusion into goethite. The initial desorption rate of P_i with KCl and H₂O decreased with increasing pre‐sorption time, whereas that of IHP was opposite. This study indicates that strong sorption on and weak desorption of IHP from iron (hydr)oxides may explain the accumulation of IHP in soils.     

真鲷仔稚鱼对实验微粒饲料中卵磷脂适宜需要量的研究

制备了4种n-3HUFA含量基本相等、卵磷脂含量分别为0、2．5％、5．0％、7．5％的实验微粒饲料，探讨了卵磷脂的含量对真鲷仔稚鱼生长、存活、体内相关成分以及对外部压力的耐受性的影响。25d的养殖试验结果表明，当真鲷仔稚鱼的实验微粒饲料中卵磷脂含量不超过5．0％时，随着实验微粒饲料中卵磷脂含量的增加，真鲷仔稚鱼的生长速度和成活率也相应增加。养殖试验结束后，对仔稚鱼体内相关成分的分析结果表明，仔稚鱼体内n-3HUFA和DHA的含量随着实验微粒饲料中卵磷脂含量的增加而增加。但当卵磷脂含量从5．0%增加到7．5％时，真鲷仔稚鱼的生长速度、成活率以及体内n-3HUFA和DHA的含量却没有相应增加，因此，真鲷仔稚鱼对实验微粒饲料中卵磷腊的适宜需要量应为5．0％左右。活力测试结果表明，当真鲷仔稚鱼的实验微粒饲料中卵磷脂的含量不超过5．0％时，随着实验微粒饲料中卵磷脂含量的增加，真鲷仔稚鱼对外部压力的耐受性增强，但是当实验微粒饲料中卵磷脂的含量从5．0％增加到7．5％时，真鲷仔稚鱼对外部压力的耐受性并没有继续增强，从而进一步说明真鲷仔稚鱼对实验微粒饲料中卵磷脂的适宜需要量应为5．0％左右。     

创新创业教育融入课堂教学的相关研究

为培养具有创新意识、创造精神和创业能力的创新型高校人才,创新创业教育融入课 堂教学已成为高校教学改革的必然趋势。文章以森保专业“森林虫害调查与标本采集制作技 术”课程为例,分析了创新创业教育融入课程教学的必要性;设计了集案例教学、专家讲座、林业 昆虫技能大赛和参与导师科研项目为一体的教学模式;阐述了创新创业教育融入教学过程中遇 到的问题。