麦根腐长蠕孢毒素对小麦苯丙氨酸氨裂解酶活性的影响

在根腐毒素浸黄化苗根和离体苗试验中，感病品种ＰＡＬ比活力显著高于对照，在一定范围内酶活性与毒素浓度成正相关，而抗病品种ＰＡＬ比活力变化不大。在旗叶圆片试验中，感病品种处理后１２ｈ，ＰＡＬ比活力明显低于对照，２４ｈ后回升，接近对照的水平；抗病品种ＰＡＬ活性除个别场合外变化不大。在酶波中直接加毒素波几乎可以完全抑制感病品种ＰＡＬ活性，对抗病品种ＰＡＬ活性也有强烈抑制作用。认为在浸根试验中未直接接触毒素的叶组织中ＰＡＬ活性变化的差异能反映品种的抗感差异，ＰＡＬ活性的相对稳定性与小麦抗根腐病有关。     

5龄桑蚕(Bombyx mori)后部丝腺氨基酸激活酶的合成动态

本研究通过以5龄桑蚕（Bombyx mori）后部丝腺重量及其组织内氨基酸激活酶蛋白含量的跟踪测定．阐明了5龄桑蚕后部丝腺蛋白质生物合成规律。结果表明，桑蚕在5龄期后部丝腺氨基酸激活酶的含量变化与后部丝腺的重量变化基本同步，均随日龄呈“S”形曲线走向。后部丝腺重量及氨量及氨基酸激活酶蛋白含量变化的“S”形曲线可用逻辑斯蒂克曲线进行描述。     

苗期叶面喷施大丽花轮枝孢激活蛋白(VdAL)对黄瓜商品苗贮藏质量的影响

为了探究大丽花轮枝孢激活蛋白(Verticilliam dahliae Allergen Asp f2-like,VdAL)干粉制剂对黄瓜商品苗贮藏质量的影响,以黄瓜幼苗为试验材料,在育苗期进行了3次不同浓度叶面喷施处理,分析贮藏前与贮藏4 d后黄瓜幼苗质量相关指标以及定植后3~6 d恢复生长指标。结果表明:1.25 g·L~(-1)大丽花轮枝孢激活蛋白处理可以显著增强黄瓜幼苗的耐贮运性,与其他处理相比,该处理浓度贮藏过程中叶绿素含量稳定,定植后恢复生长快,表明育苗期叶面喷施适宜浓度的大丽花轮枝孢激活蛋白,有利于提高黄瓜商品苗的贮藏质量。     

Inherited erythrocyte pyruvate kinase deficiency in a beagle dog

A 1-year-old, female Beagle dog with minimal exercise intolerance was found to have a persistent, severe, and highly regenerative anemia, splenomegaly, and progressive osteosclerosis. Despite near-normal in vitro erythrocyte pyruvate kinase (PK) activity, the authors diagnosed PK deficiency by demonstrating a glycolytic block at the PK step, the lack of normal R-type PK isoenzyme, and the presence of M(2)-type PK in the animal's erythrocytes. The dam had half-normal erythrocyte PK activity, which supports an autosomal recessive mode of inheritance. We conclude from our studies that close similarities exist between erythrocyte PK deficiency in Beagle, Basenji, and West Highland White Terrier dogs and that this form of PK deficiency may be more widespread than previously thought.     

甲酸乙酯和乙酸乙酯分别与辣根素原油混配对赤拟谷盗成虫的联合毒力

28℃下利用三角瓶熏蒸法测试了甲酸乙酯和乙酸乙酯分别与辣根素原油以1∶9、2∶8、3∶7、4∶6、5∶5、6∶4、7∶3、8∶2、9∶1体积比的配方对赤拟谷盗成虫的联合毒力作用。结果显示,在大多数配比中,甲酸乙酯和乙酸乙酯均对辣根素原油均具有不同的显著增效作用。最佳配比均为6∶4,共毒系数分别为165.63和176.86。在两种药剂与辣根素混配比例为8∶2和9∶1时,均表现出拮抗作用,其中辣根素与甲酸乙酯的混配共毒系数分别为62.56、57.47,辣根素与乙酸乙酯混配的共毒系数分别为40.32、43.06。     

Inhibition of plasminogen activator inhibitor-1 by small interfering RNA attenuates pulmonary fibrosis in rats

AIM: To examine the effects of silencing of plasminogen activator inhibitor-1 (PAI-1) expression by small interfering RNA (siRNA) on bleomycin (BLM)-induced rat pulmonary fibrosis. METHODS: Total 72 Wistar rats were divided into 4 groups: control, BLM, BLM+non-specific siRNA (BLM+N), and BLM+ PAI-1 siRNA (BLM+P). Pulmonary fibrosis was induced by intratracheal injection of BLM (5 mg/kg), whereas equal volume of normal saline was used in control group. After the administration of BLM or normal saline, the rats were treated with tracheal injection of PAI-1-siRNA (7.5 nmol/0.2 mL per rat) in BLM+P group, non-specific siRNA (7.5 nmol/0.2 mL per rat) in BLM+N group, and 0.2 mL normal saline in BLM group and control group, twice a week, 8 times in 28 d. On day 7, 14, and 28, the rats (n=6 at each time point) were sacrificed. The bronchoalveolar lavage fluid (BALF) from the left lung was harvested to examine the activity of PAI-1. The mRNA expression of collagen type Ⅲ, α-smooth muscle actin (α-SMA) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the middle lobe of the right lung was detected by RT-PCR. RESULTS: PAI-1 activity and the expression of collagen type Ⅲ, α-SMA and TIMP-1 were increased in BLM group on day 7, 14 and 28. Intratracheal injection of PAI-1 siRNA twice a week continuously reduced PAI-1 activity in the BALF (P<0.05),and decreased the expression of collagen type Ⅲ, α-SMA and TIMP-1 in the fibrotic lung tissues on day 7, 14 and 28. Statistical differences in the expression of collagen type Ⅲ, α-SMA and TIMP-1 between BLM+P group and BLM group at the same time point were observed. CONCLUSION: Intratracheal injection of PAI-1 siRNA twice a week continuously inhibits the expression of PAI-1. PAI-1 siRNA ameliorates BLM-induced pulmonary fibrosis by down-regulation of TIMP-1 expression.     

不同方法提取鳗鲡病原茵DNA模板的差异分析

利用吸光值、琼脂糖电泳及PCR差异扩增等指标，对酚一氯仿法、水煮法、碱裂解法3种方法制备的鳗鲡病原菌DNA模板进行了差异分析．结果表明：1）不同方法提取的病原菌模板DNA的浓度、纯度及分子质量大小存在差异，但以这3种方法提取的病原菌DNA为模板，均能成功扩增到细菌16SrD．NA和外膜蛋白的保守序列．其中，酚一氯仿提取的模板DNA浓度较低、纯度较高；水煮法和碱裂解法提取的模板DNA浓度较高、纯度较低．2）电泳显示，3种方法获得的模板DNA扩增出的16SrDNA保守序列条带均一，没有显著差异，但在外膜蛋白保守序列的扩增中却因菌种和模板提取方法的不同而存在差异．