Cinosulfuron: chemical and biological degradability, adsorption and dissipation in flooded paddy field sediment

Cinosulfuron is a sulfonylurea herbicide largely used in the extensive cultures of flooded rice in North Italy. The degradation of cinosulfuron has been investigated in sterile aqueous solutions at 30 degrees C at different pH values. It was rapidly degraded at acidic pH (half-lives 3, 9 and 43 days at pH 4, 5 and 6, respectively) while the half-life was > 1 year at pH 7 and 9. Two degradation products formed by cleavage of the sulfonylurea bridge were identified by LC-MS. Degradation by selected mixed microbial cultures tested in aerobic and anaerobic conditions was very slow and attributable to chemical hydrolysis due to the acidic pH of the cultural broths. Degradation took place in freshly collected rice field water treated for two years with cinosulfuron but, in this case also, chemical hydrolysis prevailed over microbial degradation. In contrast, in flooded sediment simulating the paddy field environment, the dissipation rate of cinosulfuron was higher than expected from chemical hydrolysis according to the pH of the system, indicating the involvement of soil microflora. Although the herbicide exhibited a reduced affinity for the sediment surfaces demonstrated by the low value of the K(f) Freundlich coefficient (0.87 on a micromolar basis), the rapid dissipation observed in the simulated paddy field should prevent its leaching to ground water.     

Usefulness of the Detached Pod Test for Assessment of Cacao Resistance to Phytophthora Pod Rot

The detached pod test by spray method (DPT-SM) was developed to facilitate the screening of cacao genotypes for resistance to Phytophthora pod rot (PPR) caused by Phytophthora palmivora. The test has been adopted in many cacao research institutes, and it is imperative that its validity be assessed. In this study, 40 genotypes from various cacao groups were selected and screened for resistance to PPR by DPT-SM and field observations. Significant variation was observed in the reactions of the selected accessions based on the disease rating scale for DPT-SM and the percentage pod rot from field observations. A correlation coefficient of 0.68 (P<0.001) was observed between the results of year-1 and year-2 field observations. However, relatively lower correlation values were obtained between year-3 and year-1 (r=0.32; P=0.041) and year-3 and year-2 (r=0.35; P=0.025) field observations. A higher level of susceptibility was observed in the third year of field observations (63%) than in the first (15%) and second (25%) years. This suggests that the predisposing factors for PPR were unstable between the years of field observations. Data obtained from the Trinidad and Tobago Meteorological Services showed that the rainfall in November of the third year was higher than the amounts of rainfall in November of the first 2 years of field observations. This month marks the beginning of the main pod harvest season (November–February) and the high rainfall in November of the third year, and the presence of large number of mature pods may account in part for the increase in PPR in the third year of field observations than in the first 2 years. This shows that absolute reliance could not be placed on a single year of field observations in determining clonal resistance to PPR. A correlation coefficient of 0.59 (P<0.001) was obtained between the result of year-2 field observations and DPT-SM. Relatively lower correlation values were observed between DPT-SM and year-1 (r=0.55; P<0.001) and year-3 (r=0.44; P=0.005) field observations. The result of DPT-SM, however, shows a higher correlation (r=0.66; P<0.001) with the average of years 1–3 field observations. This suggests that a stronger association may exist between the result of DPT-SM and the cumulative data on field observations for a period longer than 3 years. The correlation (r=0.66; P<0.001) observed in this study confirms the usefulness of DPT-SM as an effective method of assessing clonal resistance to PPR and predicting field reaction in the long term. Since field observations are labour intensive and expensive to conduct on a yearly basis, the DPT-SM offers a cheaper and effective means of assessing clonal resistance to PPR. Being a non-destructive inoculation method, the DPT-SM provides a suitable option for cacao collections in genebanks to be assessed. It is also a cost-effective method for use in cacao breeding programmes. Based on its reliability, the DPT-SM has been adopted in the CFC/ICCO/IPGRI cocoa project ‘Cocoa Germplasm Utilization and Conservation: A Global Approach’ for the assessment of cacao resistance to PPR in several cocoa producing countries.     

拮抗球胞链霉菌AM6代谢产物防治烟草赤星病的田间效果测定

 拮抗球胞链霉菌AM6代谢产物田间试验结果表明,以含有AM6拮抗物质的液剂喷雾烟草叶片,可有效防治烟草赤星病。其防效随液剂中拮抗物质的含量的增加而升高,以100倍稀释防效最好,田间防效可达80.3%;施用次数2次为佳,平均防效达64.9%,病害发生严重时,可增施1次。同时,试验过程中发现含有AM6拮抗物质的液剂对烟草无明显药害。     

Analysis of the toxic potential of Palicourea corymbifera (Müll. Arg.) Standl. in laboratory animals

Palicourea species may produce bovine toxicity. Palicourea corymbifera grows in terra firme forests within the Amazon rain forest and in Tropical America, particularly in spots that gave place to gazing areas. The lyophilized extract done with the aerial organs of P. corymbifera were analyzed in male and female mice. Results revealed a significant toxicity: LD50 was 1.10 (1.04-1.15)g/kg for male mice, and 1.05 (1.00-1.10)g/kg for female mice. Locomotion was affected as well as there were reflexes linked to environmental stimuli in addition to changes in posture. Progressive central nervous system stimulus signs such as trembling and convulsions were detected, the latter followed by the animal's death. Macroscopic histopathological exams performed on the liver, kidneys and lungs of mice submitted to necropsy did not indicate the existence of lesions. General activity of animals, measured in an open field, was reduced as a result of the administration of the extract. Duration of locomotion and rearing frequency were reduced, in opposition to an increase in the duration of immobility. Thin layer chromatography analysis showed that monofluoroacetic acid is present in the lyophilized extract, but other qualitative techniques as gas chromatography/mass spectrometry and 19F nuclear magnetic resonance showed that the MFAA was not present in the extract, and that the toxicity is related to other compound, although the toxic profile is very similar to that of MFAA. P. corymbifera was shown to be significantly toxic to laboratory animals and investigation of the possible toxic substance shall be done.     

长期不同施肥对稻田土壤有机质和全氮的影响

通过对湖南新化、宁乡、株洲、桃江、武岗5个国家级稻田肥力长期定位试验点18年的田间定位试验,研究了不同施肥方式下0-20 cm土层土壤有机质和全N含量的演变规律。结果显示,中量和高量有机肥与化肥配合处理在提高土壤有机质和全N含量方面效果明显优于单施化肥和秸秆还田处理,且随有机肥用量的增加而增加,表明有机肥与化肥配合施用是提高土壤有机质和N素肥力的重要措施。在不同施肥方式下,稻田土壤有机质总体上呈现出上升或者下降的趋势,但在不同的年份会有波动。     

小麦植株和麦田土壤莠去津残留气相色谱分析方法研究

本研究确立了乙腈振荡提取、弗罗里硅土SPE小柱净化小麦植株和麦田土壤中莠去津残留样本的前处理方法,建立了柱程序升温、GC-NPD残留样品检测方法.结果表明,优化色谱条件下,莠去津的色谱保留时间为5.97 min,在0.105～54.0 mg/L浓度范围内,莠去津浓度与其色谱峰面积在GC-NPD上线性响应良好,回归方程为Y=38.79X-120.34(R2=0.998 8).莠去津在小麦植株和麦田土壤中0.05～1mg/kg的3个水平的加标回收率均大于75％,各添加水平3次平行测定值的RSD均小于7％.其准确度和精密度均符合农药残留分析的要求.该色谱条件下仪器的最低检出量为0.38 ng,方法的最低检出浓度为0.018 mg/kg(植株)和0.021 mg/kg(麦田土壤),此检测灵敏度可以满足该农药在小麦植株和麦田土壤中残留的定量检测要求.     

不同地表覆盖方式油菜花后干物质积累与分配规律研究

为探索春油菜在西北沿山旱作区的最佳地表覆盖栽培方式,采用田间试验法,以常规露地平播为对照,研究了双垄面全膜覆盖沟播、全地面覆盖平播、膜侧沟播、半地面覆盖平播、麦秸覆盖平播等5种地表覆盖方式下春油菜的干物质积累及分配规律。结果表明,不同地表覆盖栽培方式对油菜植株各器官的干物质分配比率产生不同程度的影响,双垄面全膜覆盖沟播和全地面覆盖平播栽培方式与对照差异最大;各种地膜覆盖栽培方式均有利于油菜单株干物质积累,但不同覆膜方式效果不同。双垄面全膜覆盖沟播和全地面覆盖平播栽培方式油菜单株干物重、单位面积产量最高,秸秆覆盖未能显著提高油菜的单株产量及单位面积产量;油菜单位面积产量同油菜单株干物重呈现正相关关系,并获得最优线性回归方程;采用地膜覆盖栽培技术能有效地加快油菜植株茎秆、叶片同化物向角果的转移速度,提高油菜地上部干物质积累量和单位面积产量。其中,双垄面全膜覆盖沟播和全地面覆盖平播栽培方式比常规露地栽培增产达到30%以上,应在生产上大面积推广应用。     

准噶尔盆地南缘降水脉冲量级分布及其变化规律

对干旱区降水脉冲发生规律的分析,将为理解荒漠生态系统结构与功能提供新的视野。选择准噶尔盆地南缘的温泉、精河、乌苏、蔡家湖、奇台和巴里坤6个气象站近50多年的逐日降水资料,分析不同量级降水脉冲对年总降水频次和降水量的贡献以及变化规律。结果表明：小降水脉冲（≤5 mm）占到全年总降水频次87.5%,而大降水脉冲（>10 mm）只占到了4.3%。然而,小降水脉冲对年降水量的贡献为47.5%,大降水脉冲却贡献26.6%。近50多年来,准噶尔盆地南缘的年降水量有显著增加,但是,年降水频次的变化却不显著。这可以归结为小脉冲呈下降趋势,而大脉冲则显著增加。总之,近50多年来,准噶尔盆地南缘的降水脉冲在放大。