芝麻DNA高效提取及PAGE快速银染方法

为了快速、低成本获得高质量的芝麻基因组DNA,本实验在借鉴其他作物DNA常规提取方法基础上,简化了提取步骤,减少了试剂用量,DNA经琼脂糖电泳检测无拖尾,且OD260 /OD280在1.8～2.0之间,表明质量、纯度均较高,摸索了一套芝麻DNA高效提取方法;在常规变性聚丙烯酰胺凝胶（PAGE）银染程序基础上进行改良,减少了固定环节和试剂用量,缩短了染色和显影时间等,优化建立了芝麻PAGE快速银染方法。     

思茅松等位酶实验方法及改进

以思茅松种子的胚乳为实验材料,探索了思茅松等位酶的实验方法.采用聚丙烯酰胺垂直板凝胶电泳与水平切片淀粉凝胶电泳相结合的方法,获得所试验的13种等位酶中AAT,ADH,EST,GDH,G6PD,MDH,PGD6,PGM和SKD等9种电泳效果良好的等位酶酶谱.通过两种电泳方法的有机结合,以及对制样及上样方法的改进,大大地提高了实验效率.本研究得到的等位酶酶谱式样,为下一步思茅松种群遗传分析提供了资料.     

Identification of cultivars of Stylosanthes capitata Vog. by polyacrylamide gel electrophoresis of seed proteins

Summary A method was developed for identification of cultivars of the pasture legume, Stylosanthes capitata Vog., using electrophoretic patterns of seed proteins in polyacrylamide gels as the genotypic markers. The method can be used for accurate identification of cultivars in germplasm banks, in selecting parents for development of new varieties, and in registering new cultivars for proprietary purposes.     

聚丙烯酰胺施用方式对土壤水热及春小麦生产的影响

为了寻找聚丙烯酰胺在春小麦生产中的最佳施用方式,2010年在河套灌区,采用田间试验,以不施用聚丙烯酰胺为对照,开展了沟施、混施、撒施聚丙烯酰胺对土壤水分、土壤温度和春小麦生长状况的影响研究.结果表明:不同施用方式均提高了土壤水分含量,特别是在缺乏灌溉的开花期具有显著作用,其中撒施效果较强,沟施、混施较弱;不同施用方式降低了土壤最高温度,提高了最低温度,缩小了日温差,降低了日均温,其中撒施对地表温度影响较大,沟施对10 cm土层土壤温度影响较大;沟施、混施、撒施均提高了春小麦的总生物量及根系生物量,促进了根系向深层土壤分布,分别较对照增产9.11％、3.94％和28.82％;不同施用方式提高了水分利用效率、水分产出率和灌溉水分产出率,其中撒施效果较强,沟施较弱.在河套灌区,春小麦播种时施用聚丙烯酰胺,撒施应是最适宜的方式.     

MR水成像技术在聚丙烯酰胺水凝胶注射隆胸术后的应用和临床价值

目的探讨MR水成像技术对聚丙烯酰胺水凝胶（PAHG）注射隆乳术后的MRI表现及其应用价值。方法回顾性分析55例PAHG注射隆乳者的乳腺MRI及MR水成像技术的临床资料。结果55例110只乳房MRI常规检查显示：PAHG于T2WI呈高信号,T1WI呈低信号;PAHG周围见低信号包膜样结构影;MR水成像技术图像显示PAHG呈显著高信号,周围皮肤、皮下脂肪、乳腺腺体、肌肉、胸膜及肋骨组织均被抑制,呈低信号表现,PAHG假体可三维立体显示。结论MR水成像技术能对PAHG注射隆乳术后的性状有准确的判断,有较高的诊断价值和临床指导意义。     

受蚜虫及螨侵害的华山松过氧化物酶初步研究

利用聚丙烯酰胺凝胶电泳（PAGE），对受蚜虫侵害的华山松根、茎、叶组织内的过氧化物酶的变化及活性进行了研究。结果表明：受侵害的华山松根、茎、叶组织内的过氧化物酶活性较健康植株增强，酶带条数增多，颜色加深。受侵华山松与正常植株同一器官相比较，叶和根的变化较大，茎的变化较小。     

An improved method for discriminating between Japanese Nagoya chickens and others

Recently, we reported a method for identifying the Nagoya, a Japanese chicken breed. Here we describe an improved method suitable for use in conventional laboratories. Five microsatellite markers fixed in the Nagoya breed were amplified by multiplex polymerase chain reaction (PCR). The PCR products were digested with CEL I nuclease and electrophoresed in a ready‐made gel. The fragment‐length polymorphisms of each marker were detected clearly in the gel, and the resultant exclusion probabilities were almost equal to that in our previous data. This method can be used in laboratories without a DNA sequencer for routinely discriminating between the Nagoya breed and other chickens.     

Development of the female gametophyte in the sterile ecotype of the bolting Allium sativum L.

Manifestations of female sterility were investigated in a sterile bolting ecotype of Allium sativum (garlic). Morphological studies revealed interference during the formation of integuments around the nucellus and the lack of a micropylar channel and anatomical studies revealed disorders in female gametophyte formation. As the consequence of developmental abnormalities ovule abortion was observed. Finally, significant differences were revealed between 2D protein profiles of sterile ovules of A. sativum and fertile ovules of Allium tuberosum. Further possibilities of application of modern proteomic tools to identify proteins responsible for developmental changes in sterile ovules of A. sativum are discussed.