秦岭坡旱地不同桔梗栽培品种亲缘关系鉴别

为对秦岭坡旱地中药材桔梗品种选育及种质资源鉴定提供依据,应用聚丙烯酰胺凝胶电泳(PA GE)技术研究了不同产地三年生桔梗不同叶位酯酶同工酶(EST)的表达差异,并通过EST-PAGE 图谱迁移率（Rf）、联合系数（S）、酶谱相似度（T）综合分析方法,初步评价了 目前在商洛坡旱地栽培的不同产地桔梗品种的亲缘关系。结果表明：6种不同产地桔梗间的 亲缘关系大小顺序为：甘肃平凉＞山东淄博＞四川广元＞西安蓝田＞内蒙赤峰≈陕西商洛, 内蒙赤峰与陕西商洛桔梗疑为同一品种。     

细菌纤维素产生菌双向凝胶电泳分析技术

对细菌纤维素产生菌株———葡糖酸醋杆菌菌体蛋白的提取过程、等电点的分布及纯化方法等方面进行了探索和优化。结果表明:葡糖酸醋杆菌蛋白主要分布在pI 4~7的范围内;采用超滤浓缩后进行2D Clean-up试剂盒纯化,可以获得更优的蛋白分离效果;在考马斯亮蓝G250染色条件下,有效分离出(1037±65)个蛋白点;建立了高质量葡糖酸醋杆菌蛋白的双向凝胶电泳分析技术。     

聚丙烯酰胺对蚯蚓的毒性效应

聚丙烯酰胺作为全球应用最广泛,用量最大的水处理剂,而其排放到环境中可能会对生态环境形成潜在的威胁。该研究在人工土壤条件下,通过急性和亚急性暴露试验研究了聚丙烯酰胺和丙烯酰胺对赤子爱胜蚓存活、生长和繁殖的影响,旨在评价聚丙烯酰胺和丙烯酰胺对蚯蚓的毒性效应。结果表明,聚丙烯酰胺和丙烯酰胺对蚯蚓的半致死剂量分别为大于2000和164.01?mg/kg,聚丙烯酰胺比丙烯酰胺毒性低;在急性和亚急性毒性暴露期内,聚丙烯酰胺对蚯蚓的存活和生长无显著影响;而当丙烯酰胺浓度大于100?mg/kg时即对蚯蚓的存活和生长产生显著的影响（P＜0.05）。聚丙烯酰胺和丙烯酰胺均对蚯蚓的繁殖能力有非常显著的影响（P＜0.05）。因此残留于污泥中的聚丙烯酰胺对环境有一定的潜在风险。     

Myofibrillar proteins in white muscle of the developing African catfish Heterobranchus longifilis (Siluriforms, Clariidae)

Developmental changes in myofibrillar protein composition were investigated in the myotomal muscle of the African catfish, Heterobranchus longifilis (Clariidae), by several electrophoretic techniques. The main muscle fibres of larvae and the fast-white muscle fibres of juvenile and adult fish were found to express distinct myosin heavy chain and myosin light chain 2 (LC2) isoforms. Three myosin LC2 chains were successively detected, differing by their isoelectric points. In contrast, the alkali light chains remained qualitatively and quantitatively unchanged during fish growth. Actin, -tropomyosin, and troponin-C (TN-C) were also similar in larval, juvenile, and adult white muscle, but an additional larval tropomyosin isoform was found in the first developmental stages. Two isoforms of troponin-T (TN-T) and troponin-I (TN-I) were synthesised in the course of fish growth. Transition from the larval to the adult isoform was much faster for TN-T than for TN-I. Slow-red muscle myofibrils from adult H. longifilis showed no common component (except actin) with larval, juvenile, or adult fast-white muscle myofibrils. Red myofibrils displayed a single TN-T and a single TN-I isoform, but two isoforms of TN-C. The myofibrillar protein isoforms synthesised at any given developmental stage almost certainly reflect changes in the functional requirements of swimming muscles in the course of fish development.     

聚丙烯酰胺型共聚物对砖红壤上菜心生长状况的影响

运用不同剂量的聚丙烯酰胺型共聚物处理菜心,研究其对菜心生长和水分利用率的影响,为海南省砖红壤蔬菜栽培使用共聚物提供参考依据。结果表明,施用共聚物后各处理菜心的生长状况均优于对照（未施共聚物）,其中每667 m2用量为4 kg的处理表现最佳;当每667 m2共聚物用量在1~4 kg范围内,菜心的根重尧地上部鲜重大致随着共聚物用量的增加表现越好,但每667 m2用量为5 kg 的菜心表现不如用量为4 kg 和3 kg 的处理曰共聚物用量在每667 m2 1~5 kg 的处理在水分利用率方面分别比对照高出14.95%、27.80%、35.22%、43.52%、18.05%。     

Application of a radioimmunoassay for detection of the major internal antigen (p24) of bovine leukemia virus from cultured lymphocytes of cattle

A sensitive and specific radioimmunoassay for the major internal antigen of bovine leukemia virus has been applied to detecting this protein in cultured lymphocytes of infected cattle. The specificity inherent in this assay offers obvious advantages over a previously described syncytium induction assay for infectious bovine leukemia virus, because false positive reactions due to other viruses such as bovine syncytial virus are avoided. Investigations of various culture conditions indicated that maximal amounts of antigen had been produced after incubation for 72 hr at 37°C. Lymphocyte concentrations of 10⁶?5×10⁷ cells/ml gave satisfactory results. Tests of cultured lymphocytes from bovine leukemia virus infected or bovine leukemia virus-free cattle indicated a comparable sensitivity between the radioimmunoassay and syncytium induction assay in the detection of bovine leukemia virus infections.     

新聚丙烯酰胺凝胶电泳快速检测大豆脂氧酶缺失方法

通过对传统SDS-PAGE电泳技术中样品前处理及制胶技术的改进,开发出了一种能够清晰鉴别Lox-1与Lox-2同工酶的SDS-PAGE电泳快速检测新技术.利用新改进的方法,在289个CS8000(9)×FJ307(♂)F2杂交后代中,选拔出Lox-1,-2,-3同工酶完全缺失的个体27株,以及Lox-1,-3和Lox-...     

烟蚜两种微卫星标记银染方法的比较

实验比较了烟蚜SSR-PCR产物聚丙烯酰胺凝胶电泳两种改良银染方法,目的是为初用者在选择银染方法时提供参考。用非变性聚丙烯酰胺凝胶电泳检测烟蚜SSR-PCR的产物,结果表明银染方法二灵敏度高、简便省时,更适于对烟蚜遗传多样性的研究。     

聚丙烯酰胺对天津滨海吹填土导水性能的影响研究

为改善滨海吹填土的导水性能,加速吹填土的淋洗排盐,通过室内土柱试验,研究了不同浓度聚丙烯酰胺(PAM)溶液施用对滨海吹填土入渗性能和淋盐能力的影响,结果表明：与对照处理相比,0.05、0.2、0.3、0.6、1.2、2.4 g/L PAM溶液施用均能有效改善滨海吹填土土壤结构,显著提升吹填土导水性能和淋洗脱盐能力,降低吹填土pH,增加土壤水分含量。淋洗初期,PAM施用浓度越高,吹填土入渗速率越大,随时间的延长,入渗速率降低;出水稳定后,0.6 g/L PAM处理入渗速率最大,达0.059 cm/h,为对照的1.64倍;淋洗267 h后,施用PAM处理土壤含盐量均低于1.0 g/kg,为非盐渍土,0.6 g/L处理最低,仅为0.32 g/kg,而对照处理含盐量为4.82 g/kg,属于重度盐渍土。可得出结论对于天津滨海吹填土,300万分子量的阴离子型PAM最佳施用浓度为0.6 g/L。