口蹄疫病毒2c基因的克隆及表达

从O型口蹄疫病毒China98株病毒材料中克隆到了非结构蛋白2C的基因，并成功地将该基因在大杆菌中进行了表达，为建立以基因工程产品为抗原，能区分人工免疫和自然感染动物的检疫方法提供也技术和材料条件。 sri     

维生素C和中草药添加剂对夏季种公鸡精液污染率和种蛋受精率的影响

为研究维生素C和中草药添加剂对夏季种公鸡精液污染率和种蛋受精率的影响,选取40周龄体重相近的安卡红种公鸡30只,随机分为3组,分别为维生素C组、中草药组和对照组,对照组喂基础日粮,试验组分别在基础日粮中添加250 mg/kg的维生素C和1.5％的中草药。结果表明：维生素C组和中草药组的平均精液污染率比对照组降低了24.34％和17.34％,且差异显著（P＜0.05）;维生素C组和中草药组的受精率分别比对照组提高了2.17%和4.35%,且差异显著（P＜0.05）。说明在热应激条件下,在日粮中添加适量的维生素C和中草药可显著降低种公鸡精液污染率,改善精液品质,进而提高种蛋受精率。     

基于Cortex-M4内核的μCOS-Ⅱ移植

针对TI公司新推出的基于Cortex-M4内核的TM4C123G高性能低功耗芯片,详细介绍了嵌入式开源实时操作系统μCOS-Ⅱ在芯片上的移植方法。根据移植的需求,首先介绍了芯片的一些基本功能以及相关的软件开发环境,然后结合芯片的固有特性以及μCOS-Ⅱ移植的需求,使用C语言和汇编语言修改了相关的源文件,并详细阐述了修改的原因。     

Effects of vitamin C supplementation on growth performance and antioxidant status of layer ducklings

The study was conducted to evaluate the effects of increasing dietary vitamin C supplements on growth performance, antioxidant status and humoral immunity of layer ducklings. The results showed that the body weight and daily body weight gain of ducklings increased (p < 0.05) with increasing dietary vitamin C supplementations and reached a maximum at 400 mg vitamin C/kg feed. The dietary vitamin C supplementations reduced the malondialdehyde concentration (p < 0.05) and increased the activities of superoxide dismutase and glutathione peroxidase (p < 0.05) in serum and liver of ducklings at day 5 and 28. Additionally, feeding 400 mg/kg or 800 mg vitamin C/kg feed increased IgM and IgA concentrations in sera (p < 0.05) and serum IgG concentrations increased (p < 0.05) following supplementation in concentrations of 150-800 mg vitamin C/kg feed. In conclusion, the results suggested that dietary vitamin C supplements of 400 mg/kg feed provide optimal effects on growth performance, antioxidant status and parameters of humoral immunity.     

Effect of experimental endotoxemia on thrombelastography parameters, secondary and tertiary hemostasis in dogs

Background: Thrombelastography (TEG) and indicators of secondary and tertiary hemostasis might be altered in dogs with endotoxemia. Hypothesis: Endotoxemia influences measures of coagulation in dogs. Animals: Ten healthy cross‐bred dogs. Material and Methods: Prospective laboratory study between controls (n = 5) receiving 0.9% saline IV and the study group (n = 5) treated with low‐dose lipopolysaccharide (0.02 mg/kg IV). Physical examination and sampling for measurement of leukocytes, platelets, and coagulation variables were performed at time points 0, 1, 4, and 24 hours. Coagulation variables included kaolin‐activated TEG, 1‐stage prothrombin time (OSPT), activated partial thromboplastin time (aPTT), fibrinogen, factor VIII, antithrombin, protein C, protein S, activated protein C (APC)‐ratio calculated from aPTT with and without presence of APC), and D‐Dimers. Results: Endotoxemia‐induced clinical signs included lethargy (n = 5/5), diarrhea (n = 4/5), emesis (n = 4/5), and abdominal pain (2/5). After 1 hour there was severe leukopenia (2.5 ± 0.7 × 10⁹/L; mean ± SD, P < .0001) and a 2.2‐fold increase in D‐Dimers (0.81 ± 0.64 mg/L, P < .0001). After 4 hours there was hyperthermia (40.3 ± 0.4°C, P < .0001) and increases in OSPT (10.5 ± 2.7 seconds, P < .0001), aPTT (16.7±5.2 seconds, P= 0.002). A significant decrease in fibrinogen (1.5±1.0 g/L, P= 0.001), protein C (31 ± 33%, P <.0001), protein S (63 ± 47%, P < .0001), TEG α (58 ± 19, P= .007), and TEG maximal amplitude (50 ± 19 mm, P= .003) was seen compared with the controls. APC‐ratio rose significantly (2.5 ± 0.2, P < .0001) without exceeding the reference interval (n = 4/5). Conclusion and Clinical Importance: D‐Dimers are the earliest indicator for endotoxemia‐associated coagulation abnormalities followed by decreased protein C concentration. APC‐ratio and TEG were not good screening variables.     

橡实象虫等25种昆虫线粒体COⅠ基因的遗传多样性及系统进化分析

橡实象虫(Curculio arakawai)是象甲科中危害柞树种子和嫩芽的重要害虫。为探讨利用线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因的特定区段作为DNA条形码快速准确识别橡实象虫的可行性,克隆了3个橡实象虫线粒体COⅠ基因5'端长度约556 bp的片段(GenBank登录号:JN258957,JN258958,JN258959),并与鞘翅目、鳞翅目、双翅目共24种昆虫的同源片段进行碱基组成多样性和系统进化分析。序列分析结果显示:橡实象虫与其它24种昆虫的COⅠ基因序列呈现丰富的遗传多样性,碱基位点的变异率为48.2%,序列平均差异为19.7%;COⅠ基因序列的AT含量(69.9%)明显高于GC含量(30.1%),存在显著的AT使用倾向性;碱基替换模式颠换大于转换,碱基替换数与物种两两遗传距离呈明显的线性关系;COⅠ基因序列的碱基变异速率较快的位点主要集中在序列中间区域的210～390 bp。基于COⅠ基因序列构建的NJ、ME和MP系统进化树均显示橡实象虫与鞘翅目象甲科(Curculionidae)昆虫的进化关系最近,聚在一起形成一个分支,鳞翅目等其它昆虫聚在一起形成另外一个分支;橡实象虫与鞘翅目象甲科Kyklioacalles属昆虫间遗传距离最小,与鳞翅目丝角蝶科Macrosoma sp.属昆虫间的遗传距离最大。根据COⅠ基因序列的多样性,能将橡实象虫明显地与其它昆虫区分开。     

Outbreak of Brucellosis in Domestic Elk in Korea

Seven of 18 elk on a deer farm were found by the official Rose‐Bengal agglutination test (RBT) and tube agglutination test to be brucellosis reactors/suspects. Evaluation with the competitive ELISA (C‐ELISA) and the fluorescence polarization assay (FPA) tests revealed that six and five sera were positive respectively. The seven reactors/ suspects were slaughtered and their blood and tissues were collected. Brucella species could be isolated from three of the slaughtered animals, with nine isolates being obtained from the popliteal, supramammary and submandibular lymph nodes, vaginal discharge, mammary tissue and spleen. Brucella genus‐specific PCR based on 16S rRNA and AMOS‐PCR, which is specific for differential Brucella species, revealed that all nine isolates were Brucella abortus. These nine were further confirmed to be B. abortus biovar 1 by classical biotyping scheme assays. This is the first report of an outbreak of brucellosis in domestic elk in Korea. Our observations suggest that deer should be included in the routine Brucella surveillance programme for the effective control and prevention of brucellosis in Korea.     

C肉毒杀鼠素在高寒草甸地区灭鼠技术的应用

综述C肉毒杀鼠素草原灭鼠应用技术，充分肯定了C肉毒杀鼠素毒力强、适口性好、对非靶动物毒性低、作用缓慢、无二次中毒、易降解和适合规模灭鼠的特点，为鼠害防治提供了新的思路。     

荒漠草原“科尔沁型”华北驼绒藜旱作技术研究

“科尔沁型”华北驼绒藜是优良的旱生牧草,但其果实带毛、粘连,不适宜机械播种,而且种子发芽要求较高的温度、湿度,在荒漠草原地区直接播种,建立旱作人工草地难度很大。先进行育苗后,采用机械化植苗技术植苗,在撂荒、退耕地建立旱作人工草地,具有成活率高、速度快、操作性强、成本低、简单易行的特点,可实现大规模机械化作业,效益显著,为荒漠草原地区生态建设探索出了一条新的有效途径,可在北方干旱、半干旱地区大力推广应用。