爱媛28’香橙砧与枳砧在南平市引种表现与评价

2017年引进早熟杂柑品种‘爱媛28’香橙砧和枳砧苗分别在大棚设施和露天进行试种,栽培4年调查比较两个砧木组合的生长表现、丰产性与果实品质,结果表明：‘爱媛28’香橙砧树表现生长势强,冠幅、树高、茎粗、结果枝、秋梢等生长量指标显著大于枳砧树,3a树初产1436 kg/667㎡,4a树2523kg/667㎡,显著高于枳砧4a树初产1809kg/667㎡;大棚种植香橙砧4a树果实10月下旬TSS11-12%,12月中旬TSS可达15%,与枳砧相当,可滴定酸比枳砧略高,两者固酸比均达18以上,口感风味极佳,两者单果重、果形指数、果皮厚度、可食率无显著性差异;未密封大棚4a树体经受2020年寒冬极端低温考验无明显冻害,抗旱抗寒能力中等,易感黑点病和流胶病,大棚种植能很好预防黑点病。综合评价爱媛28枳砧和香橙砧均适合在南平市种植,香橙砧比枳砧早期丰产性稳产性好,收益更高,香橙砧‘爱媛28’设施种植较枳砧更具优势。     

荒漠草原“科尔沁型”华北驼绒藜旱作技术研究

“科尔沁型”华北驼绒藜是优良的旱生牧草,但其果实带毛、粘连,不适宜机械播种,而且种子发芽要求较高的温度、湿度,在荒漠草原地区直接播种,建立旱作人工草地难度很大。先进行育苗后,采用机械化植苗技术植苗,在撂荒、退耕地建立旱作人工草地,具有成活率高、速度快、操作性强、成本低、简单易行的特点,可实现大规模机械化作业,效益显著,为荒漠草原地区生态建设探索出了一条新的有效途径,可在北方干旱、半干旱地区大力推广应用。     

Vitamin C enhanced myocardial differentiation of dedifferentiated fat cells

AIM: In order to observe the myocardial differentiation capacity of the dedifferentiated fat (DFAT) cells treated with vitamin C in vitro. METHODS: DFAT cells were dedifferentiated from the mature rat adipocytes with ceiling adherent culture. The DFAT cells of passage 3 were used in the study. Vitamin C and/or neonatal rat heart tissue lysate were added into the culture medium to induce myocardial differentiation for 3 weeks. The cell morphology was observed under microscope. The myocardial-specific markers, such as cTnT, GATA-4 and NKx2.5, were examined by the methods of immunofluorescence, PCR and Western blot. RESULTS: Mature rat adipocytes dedifferentiated into fibroblast-like DFAT cells after ceiling adherent culture. The DFAT cells spontaneously differentiated into cardiomyocyte-like cells under normal culture condition with a low incidence. After treated with neonatal rat heart cell lysate, the DFAT cells became cardiomyocyte-like cells that had bigger size, longer shape and myotubule-structure. The expression of cTnT, GATA-4 and NKx2.5 was remarkably increased at both mRNA and protein levels as compared with the normal cultured DFAT cells. The expression of cTnT, GATA-4 and NKx2.5 was further increased in DFAT cells after treating with vitamin C. No spontaneous beating cell was observed. CONCLUSION: Vitamin C enhances the differentiation of DFAT cells into cardiomyocyte-like cells.     

Sublytic C5b-9 induces protective autophagy in cultured podocytes

AIM: In podocytes, autophagy occurs at a high basal level and dysregulated autophagy is associa-ted with a variety of podocytopathies. This paper is to investigate the role of autophagy in sublytic C5b-9-induced podocyte injury. METHODS: Sublytic complement C5b-9 stimulation was used as an in vitro model. Autophagosomes were confirmed using monodansylcadaverine (MDC) staining. Immunoblotting was used to measure the change of autophagy-related markers. Cellular morphological changes were observed by Wright-Giemsa staining. Immunofluorescence staining and confocal microscopy were used to detect the expression and distribution of nephrin. The cell viability was assessed by methylthiazol tetrazolium (MTT) assay. The cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/PI staining. RESULTS: For ensuring sublytic complement injury, the maximal amounts of anti-podocyte antiserum and 160×-diluted normal human serum were used without inducing cell lysis (defined as >5% LDH release). Sublytic C5b-9 promoted autophagy of podocytes in vitro. The proautophagic effect of sublytic C5b-9 manifested in the form of accumulated MDC-labeled vesicles and enhanced the expression of LC3-Ⅱ. Autophagy inhibitor 3-methyladenosine (3-MA) promoted sublytic C5b-9-induced podocyte morphological abnormalities. Compared with the sublytic C5b-9-injured podocytes, 3-MA exposure further decreased the expression of nephrin. 3-MA enhanced sublytic C5b-9-induced podocyte apoptosis. CONCLUSION: Sublytic C5b-9 attack induces autophagy, which may play a protective role against complement-mediated podocyte injury.     

Using composted wheat residue as a growth medium in culture of tomato

A greenhouse experiment was carried out to investigate the effects of different growing media on some properties of tomato (Lycopersicom esculentum L. cv. Falcato). Four growing media including wheat residue, composted wheat residue, coco peat + perlite, and coco peat + soil were applied in pot experiment with completely randomized design with three replications. The results showed that fruit yield, chlorophyll, and vitamin C contents of tomatoes grown in the composted wheat residue and coco peat + perlite treatments were significantly greater than the other growth media. Fresh weight of root and inflorescence were higher in the tomatoes cultivated in the composted wheat residue pots than the other media. Higher internode length and nutrient concentrations in leaf and fruit at the coco peat + perlite treatment were obtained as compared to the other growth media treatments. According to the results, it can be concluded that the coco peat + perlite can be substituted for by composted wheat residue for the cultivation of tomato.     

田间施用硝化抑制剂DMPP对小青菜贮藏过程中硝酸盐与Vc含量的影响

采用田间施药方法,研究了施用硝化抑制剂3,4-二甲基吡唑磷酸盐(3,4-dimethylpyrazolephosphate,DMPP)对冰箱贮藏条件下小青菜可食部分硝酸盐、维生素C(Vc)含量变化的影响。结果表明,在贮藏过程中,施与不施DMPP2种处理小青菜硝酸盐含量呈现先降低后略有升高的趋势,Vc含量呈降低的趋势;DMPP在贮藏前期能够有效地降低硝酸盐含量,延缓Vc含量的降低。贮藏2d后,DMPP处理小青菜硝酸盐、Vc含量分别降低了74.4和9mg·kg-1,而对照处理分别降低了54.4和50mg·kg-1,处理间硝酸盐、Vc降幅差异均达5%显著水平。贮藏2～4d中,DMPP处理硝酸盐、Vc含量分别降低了20.1和91mg·kg-1,而对照处理分别降低了144.7和84mg·kg-1,处理间硝酸盐含量降幅差异达5%显著水平。贮藏4～6d中,对照处理硝酸盐含量升高了60.6mg·kg-1,DMPP处理升高了76.2mg·kg-1,Vc含量分别降低23和117mg·kg-1,且处理间降幅差异达5%显著水平。蔬菜贮藏时间不要过长,以不超过4d为宜。     

Benzophenone glycosides and epicatechin derivatives from Malania oleifera

A new benzophenone C-glycoside, malaferin A (1), and two new epicatechin derivatives, malaferin B (2) and malaferin C (3), together with five known compounds were isolated from Malania oleifera. In addition, (-)-epicatechin-3-O-benzoate (6) was isolated for the first time from a natural resource. Structures of 1-3 were determined on the basis of their spectroscopic methods, including 1D and 2D NMR techniques. All of the compounds were evaluated for anti-HIV activities.     

Analysis of the structure of lignin-carbohydrate complexes by the specific13C tracer method

In the present study the specifically¹³C-enriched lignin precursors of biosynthesis (i.e., coniferin-[side chain–¹³C], coniferin-[side chain-–¹³C] and coniferin-[side chain-–¹³C]) were synthesized and administered exogeneously to ginkgo shoots (Ginkgo biloba L.) to obtain¹³C-enriched lignin-carbohydrate complexes (LCCs). The specifically¹³C-enriched LCCs were isolated from the newly formed xylem of ginkgo shoots administered with the¹³C-enriched precursors and degraded by enzymes. Lignin-rich fractions, so called enzyme-degraded LCCs (EDLCCs), were obtained. By determining their¹³C-NMR spectra, information related to the chemical structure of lignin building units and linkages between phenylpropane units of lignin and carbohydrates were obtained. It was found that these precursors were incorporated in natural lignin successfully. Three lignin-carbohydrates linkages (i.e., ether type, ester type, ketal type) were found at the C -position of the side chain of phenylpropane units in ginkgo LCC. No lignin-carbohydrate bond at the C- or C-position of the lignin side chain was observed in the¹³C-NMR spectra of the¹³C-enriched LCCs. This fact indicates that a specific¹³C tracer technique can be useful in NMR study of the chemical structure of LCCs.Part of this paper was presented at the 5th Pacific Polymer Conference, Kyongju, Korea, October 1997     

2个荔枝品种花芽分化期碳氮营养的变化

比较分析妃子笑和鹅蛋荔（Litchi chinensis Sonn．cv．Feizixiao＆Edan）2个荔枝栽培品种花芽分化过程中不同方位、不同部位枝条顶芽和花穗中碳氮化合物（C／N）比的动态变化。结果表明：荔枝花芽分化期间不同方位、小同部位、不同品种荔枝花芽分化期问枝条顶芽中在C／N比差片均达极显著;妃子笑的C／N比在前5个阶段均高于鹅蛋荔,只在第6阶段（开花期）低于鹅蛋荔;C／N比反映枝条顶芽和花穗的营养积累情况,比值较高有利于荔枝花芽分化。