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991.
【目的】细胞自噬和凋亡存在着相互制约,p38MAPK信号通路作为细胞凋亡的主要调控通路之一,也对细胞自噬存在促进和抑制的双重作用。已有研究表明,促性腺激素抑制激素(gonadotropin-inhibitory hormone , GnIH)对细胞自噬与凋亡均有影响,但作用机制尚不明确。故探究GnIH通过p38MAPK信号通路对猪卵巢颗粒细胞(pGCs)自噬与凋亡的影响及其机理,为解决母猪的产子率以及同期发情等问题提供参考。【方法】min、10 min、30 min、60 min、90 min)分组,用Western blot检测猪卵巢颗粒细胞p38与p-p38的蛋白表达量变化;2、验证GnIH对p38MAPK信号通路的影响:按(空白对照、GnIH、p38激活剂(U-46619)、U-46619+GnIH)分组,用Western blot检测p38与p-p38的蛋白表达量变化;3、探究不同浓度GnIH对自噬和凋亡的影响:按(空白对照、10 -6mol·L -1 GnIH、10 -8mol·L -1 GnIH、10 -10mol·L -1 GnIH、10 -12mol·L -1 GnIH)分组,用Western blot检测自噬与凋亡标志性蛋白的表达量变化;4、验证不同浓度GnIH通过p38信号通路对自噬和凋亡的影响:将细胞分成6组(空白对照、U-46619、U-46619+10 -6 mol·L -1 GnIH、U-46619+10 -8mol·L -1 GnIH、U-46619+10 -10mol·L -1 GnIH、U-46619+10 -12mol·L -1 GnIH),用Western blot检测自噬与凋亡标志性蛋白的表达量变化。【结果】1. GnIH孵育10 min后,显著降低p38与p-p38的蛋白表达量(P<0.05),提示,GnIH对p38MAPK信号通路的最佳作用时间为10 min;2. U-46619显著促进pGCs的p38磷酸化水平(P<0.05),GnIH显著抑制pGCs的p38磷酸化水平(P<0.05),提示,U-46619使p38MAPK信号通路活化,GnIH对p38MAPK信号通路的活化有抑制作用;3. 当 GnIH的浓度为10 -6 mol·L -1时,pGCs的自噬和凋亡水平显著升高(P<0.05),随着GnIH浓度的降低,pGCs的自噬水平逐渐升高(P<0.05),pGCs的凋亡水平逐渐降低(P<0.05),提示,高浓度GnIH可以促进自噬和凋亡,随着GnIH浓度的降低,自噬水平逐渐升高,而凋亡水平逐渐下降;4. 加入U-46619后,GnIH使pGCs的自噬显著上调(P<0.05),并且使pGCs的凋亡显著下调(P<0.05),提示,不同浓度GnIH通过p38MAPK信号通路影响pGCs的自噬和凋亡。【结论】GnIH可能通过抑制p38MAPK信号通路的活化,上调pGCs的自噬,减少pGCs的凋亡。 相似文献
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994.
AIM: To investigate the effect of microRNA-24-3p (miR-24-3p) on the viability and apoptosis of esophageal cancer cells. METHODS: The expression of miR-24-3p and KLF6 mRNA in the esophageal cancer cells TE11, Eca109 and EC9706 were detected by RT-qPCR. The protein expression of KLF6 was determined by Western blot. EC9706 cells were transfected with anti-miR-24-3p and KLF6 siRNA. The cell viability was measured by MTT assay, the apoptotic rate was analyzed by flow cytometry, and the proliferation, apoptosis and IL-6/STAT3 signaling pathways related proteins were determined by Western blot. The level of IL-6 was measured by ELISA. The dual luciferase reporter gene assay was used to verify the relationship between miR-24-3p and KLF6. RESULTS: The levels of miR-24-3p were up-regulated in the esophageal cancer cells TE11, Eca109 and EC9706 (P < 0.05), and the expression of KLF6 at mRNA and protein levels was down-regulated (P < 0.05). Knock-down of miR-24-3p expression inhibited the cell viability, induced apoptosis, and inhibited the protein levels of CDK4, cyclin D1, CDC25A, p-STAT3, Bcl-2 and IL-6, and promoted the protein expression of caspase-3 and Bax in EC9706 cells. CONCLUSION: miR-24-3p targets KLF6 gene to affect the viability and apoptosis of esophageal cancer cells by regulating IL-6/STAT3 signaling pathway. 相似文献
995.
DONG Yan DU Kai-xian JIA Tian-ming WANG Jun ZHANG Yan LI Lin GUAN Jing CHEN Hao 《园艺学报》2020,36(3):501-506
AIM: To explore the mechanism of microRNA-301a-3p (miR-301a-3p) regulating connexin 43 (Cx43) expression in the rat astrocytes. METHODS: miR-301a-3p agomir, miR-301a-3p antagomir and microRNA negative control (miR-NC) were synthesized and transfected into the astrocytes. The protein expression of Cx43 was determined by Western blot. The recombinant vectors wt-pEZX-MT05-Cx43 and mut-pEZX-MT05-Cx43 were constructed. The target gene of miR-301a-3p was validated by dual-luciferase reporter assay. The expressional vector pcDNA3.1-Cx43 was constructed and the biological activity regulation of miR-301a-3p on astrocytes was analyzed by restore experiment. RESULTS: The results of Western blot showed that the Cx43 expression was inhibited by miR-301a-3p agomir transfection (P <0.05). The results of dual-luciferase reporter assay showed that miR-301a-3p bound to 3′-UTR of Cx43, thus regulating expression of Cx43 negatively. Transfection with recombinant vector pcDNA3.1-Cx43 without 3′-UTR of Cx43 into astrocytes resulted in the restoration of the negative function of miR-301a-3p on Cx43 expression, also induced the apoptosis of astrocytes. CONCLUSION: miR-301a-3p binds to 3′-UTR of Cx43 mRNA in the rat astrocytes, thus regulating expression of Cx43 negatively. 相似文献
996.
为研究不同土壤容重下红壤土与黄土中水分与硝态氮垂直一维入渗运移特性差异,提高红壤与黄土地区水肥利用效率,以江西壤黏土与西安粉壤土为研究对象,采用垂直一维入渗方式模拟土壤容重对2种土壤中水分及硝态氮垂直运动规律的影响.结果表明:江西壤黏土与西安粉壤土的湿润锋运移距离及入渗速率均与土壤容重呈负相关;灌水结束时与再分布1 d后,2种土壤的含水率均与土壤容重呈负相关,西安粉壤土的含水率略大于江西壤黏土;土壤容重越大,硝态氮越集中于深层土壤中且其峰值越高,再分布过程中峰值下降,其中容重为1.25,1.35,1.40 g/cm3的峰值下降较大.灌水结束时土壤容重对40~50 cm土层内的硝态氮质量比在0.05水平下均具有统计学意义;再分布1 d后,2种土壤在0~30 cm土层内的硝态氮质量比相差甚小,在30~60 cm土层内的硝态氮质量比均较高,但西安粉壤土的硝态氮质量比更高.故江西壤黏土中硝态氮更容易淋溶,而西安粉壤土的持水持肥性较好. 相似文献
998.
AIM:To investigate the expression and clinical significance of microRNA-139-3p (miR-139-3p) in the apoptosis model of cardiomyocytes induced by hypoxia. METHODS:Under normal and hypoxic conditions, the expression of miR-139-3p in neonatal rat cardiomyocytes was detected by RT-qPCR. miR-139-3p inhibitor and miR-139-3p inhibitor negative control were transfected into the primary neonatal rat cardiomyocytes. The transfected cardiomyocytes were cultured in closed anoxic box (95% N2 and 5% CO2) at 37℃ for 12 h. Flow cytometry and Western blot were used to determine the apoptosis of cardiomyocytes. RESULTS:After hypoxia for 12 h, the expression level of miR-139-3p and the apoptotic rate of the cardiomyocytes in hypoxia group were significantly higher than those in normal group (P<0.05). Moreover, compared with the miR-139-3p inhibitor negative control group, the apoptotic rate of the cardiomyocytes was significantly decreased in miR-139-3p inhibitor group (P<0.05). CONCLUSION:The expression of miR-139-3p is signi-ficantly increased in apoptotic neonatal rat cardiomyocytes induced by hypoxia. Inhibition of miR-139-3p expression reduces hypoxia-induced apoptosis of cardiomyocytes. 相似文献
999.
AIM:To investigate the changes of hyaluronan and proteoglycan link protein 1 (HAPLN1) expression before and after resistance to methotrexate (MTX) in human colorectal cancer HT-29 cells and its effect on this drug resistance, and to explore the molecular mechanism in the process. METHODS:The drug-resistant HT-29/MTX cells were established by stepwise exposure of the cells to MTX, and then the HT-29/MTX cells were stably transfected with specific shRNA interference plasmid vectors targeting HAPLN1 and multidrug resistance-associated protein 2 (MRP2). The mRNA expression levels of HAPLN1 and MRP2 were measured by RT-PCR. CCK-8 assay was used to detect the viability of HT-29/MTX cells. The apoptosis rate was analyzed by flow cytometry. The protein levels of HAPLN1, MRP2, IκB kinase (IKK) α/β, p-IKKα/β (Ser176/Ser177), p65 and p-p65 (Ser536) were determined by Western blot. RESULTS:The HT-29/MTX cells had significantly higher mRNA and protein levels of HAPLN1 and MRP2 than HT-29 cells (P<0.05) with resistant factor of 463.756. HAPLN1 and MRP2 gene silencing significantly increased the cytotoxicity and apoptosis of HT-29/MTX cells induced by MTX (P<0.05). The IC50 value was decreased from 15.304 μmol/L to 6.119 μmol/L and 7.801 μmol/L, respectively, and their reversal folds were 2.501 and 1.962, respectively. Silencing of HAPLN1 and IKK inhibitor IKK16 inhibited the phosphorylation of IKKα/β and p65 (P<0.05), and down-regulated the protein level of MRP2 in the HT-29/MTX cells (P<0.05). However, IKK16 did not affect the protein level of HAPLN1 in the HT-29/MTX cells.CONCLUSION:Knock-down of HAPLN1 gene expression reverses the resistance to MTX in human colorectal cancer HT-29/MTX cells possibly by blocking the IKK/p65 signaling pathway and thus down-regulating the expression of MRP2. 相似文献
1000.