日本晚樱同源异型基因PrseAP3的克隆及其在单瓣与重瓣花中的表达分析

用同源克隆的方法和3′RACE技术,从日本晚樱(Prunus lannesiana)中分离得到了1个AP3同源基因PrseAP3的cDNA全长,GenBank登录号为JF710371。其cDNA全长977bp,包括1个编码235个氨基酸共708bp的开放阅读框。序列比对和分子系统发生分析表明,PrseAP3是拟南芥的AP3同源基因,其蛋白质的C末端具有2个保守基元:PI-derived模体和paleoAP3模体,属B类转录因子中paleoAP3进化系。半定量RT-PCR分析表明,PrseAP3主要在单瓣日本晚樱品种大岛的萼片、花瓣和雄蕊中表达;在重瓣品种一叶的花瓣、雄蕊和叶化心皮中表达。其表达组织特异性在2个品种间表现出明显的差异,并与拟南芥的AP3基因有一定的差别。     

Lipid-induced p62dok up-regulation enhances hepatic gluconeogenesis

AIM:To investigate the potential role of p62^dok in the regulation of hepatic gluconeogenesis. METHODS:The expression of p62^dok, insulin signaling transduction, and hepatic gluconeogenesis were investigated in the liver tissues of mice treated with high-fat diet(HFD) and in cultured mouse hepatocytes treated with free fatty acid(FFA). The experiments of gene silencing and overexpression were conducted to observe the effects of p62^dok on insulin signal transduction and hepatic gluconeogenesis in cultured mouse hepatocytes. Western blotting was used to detect the protein levels and the phosphorylation statue. RESULTS:The increased p62^dok levels were found in the liver tissues from HFD-treated mice and FFA-treated hepatocytes. Meanwhile, phosphorylation of Akt and forkhead box O1 protein(FoxO1) was were decreased and the expression of glucose-6-phosphatase(G6Pase) and phosphoenolpyruvate carboxykinase(PEPCK) was increased. Silencing of p62^dok in cultured hepatocytes treated with FFA induced the increase in phosphorylation of Akt and FoxO1, and decrease in the protein levels of G6Pase and PEPCK. CONCLUSION:Up-regulation of p62^dok induced by HFD or FFA enhances hepatic gluconeogenesis via inhibiting insulin signal transduction.     

内毒素对肝脏p53表达及Ca^2＋浓度的影响

为探讨内毒素在致大鼠肝损伤过程中对肝脏p53表达及ca^2＋浓度的影响,将48只SD大鼠随机分2组：Ⅰ组为NS对照组;Ⅱ组为ET致病组,2组大鼠分别于第3、4、8、12h每组各处死6只,采集肝脏分别用于p53表达的Western-blotting检测和ca^2＋浓度的FCM检测。结果显示,Ⅱ组p53的表达明显高于Ⅰ组（P＜0．01）,且Ⅱ组一直呈上升趋势;Ⅱ组Ca^2＋的荧光指数明显高于Ⅰ组（P＜0．01）,且一直呈上升趋势。结果表明,ET能有效上调肝细胞p53蛋白的表达及Ca^2＋浓度。     

鸡、鹌鹑及其杂交禽胚胎肌肉发育过程中MSTN和p21基因的表达规律

旨在研究MSTN和p21基因在鸡、鹌鹑和杂交禽胚胎肌肉发育过程中的表达规律,比较这2个基因在杂交禽与亲本鸡和鹌鹑中的差异表达,探讨MSTN和p21基因与肌肉发育的关系。通过人工受精获得鸡(♂)与鹌鹑(♀)杂交禽蛋,与鸡蛋、鹌鹑蛋按照鸡标准孵化条件同批入孵,采集第7～17天活胚胸肌组织,应用实时荧光定量PCR技术检测MSTN和p21基因在3个物种中每一天mRNA的相对表达量。MSTN和p21基因在胚胎肌肉发育的第7～17天均有表达,这2个基因mRNA的表达在鸡胚中第9天到达第1次峰值,在鹌鹑中第7天到达第1次峰值,后都随胚胎的发育表达水平上升,并维持相对稳定的高水平表达。杂交禽的表达规律与鸡一致,也在第9天达到峰值,而p21mRNA表达极显著高于鸡和鹌鹑(P<0.01)。MSTN mRNA表达规律与成肌细胞退出细胞周期的时间规律一致;在胚胎肌肉发育过程中,MSTN基因特异性上调p21的表达。     

细叶朱蕉扦插繁殖技术研究

在塑料大棚中,试验研究不同扦插基质、不同植物生长调节剂及浓度、不同扦插时间对细叶朱蕉扦插成苗率的影响。结果表明:细叶朱蕉扦插繁殖以4月中旬—6月中旬和10月中旬为最适宜时期;以河沙+黄心土(1∶1)为扦插基质,插穗基部经GGR6#1000 mg·L-1浸泡2 min,生根效果较佳,平均扦插成苗率可达87%。     

Effects of p204 expression on expression of p21 and proliferation of vascular smooth muscle cells in rats

AIM: To observe the effect of interferon-inducible protein 204 (p204) on the expression of p21 and proliferation of vascular smooth muscle cells (VSMCs) in rats. METHODS: Interferon alpha (IFN-α) and small interference RNA (siRNA) targeting p204 gene ( Ifi204 ) was used to intervene cultured VSMCs in vitro instantaneously, then the cell vitality was determined by MTT assay to reflect the cell proliferation. The cell cycle was analyzed by flow cytometry. The expression of p204 and p21 at mRNA and protein levels was determined by semi-quantitative RT-PCR and Western blotting. RESULTS: In rat VSMCs, IFN-α induced the increase in the expression of p204 at mRNA and protein levels, reduced the cell vitality and the G₁/S phase transition, and up-regulated the expression of p21 at mRNA and protein levels. Transfection of Ifi204 siRNA restrained the expression of p204 and p21, increased the cell vitality and promoted the G₁/S phase transition. CONCLUSION: The expression of p204 restrains the proliferation of rat VSMCs, probably by activating the expression of p21.     

内毒素对肝脏p53表达及Ca2+浓度的影响

为探讨内毒素在致大鼠肝损伤过程中对肝脏p53表达及Ca2+浓度的影响,将48只SD大鼠随机分2组:Ⅰ组为NS对照组;Ⅱ组为ET致病组,2组大鼠分别于第3、4、8、12h每组各处死6只,采集肝脏分别用于p53表达的Western-blotting检测和Ca2+浓度的FCM检测。结果显示,Ⅱ组p53的表达明显高于Ⅰ组(P<0.01),且Ⅱ组一直呈上升趋势;Ⅱ组Ca2+的荧光指数明显高于Ⅰ组(P<0.01),且一直呈上升趋势。结果表明,ET能有效上调肝细胞p53蛋白的表达及Ca2+浓度。     

p27基因在宫颈癌组织中的表达及其意义

目的：探讨肿瘤抑制基因p27在宫颈癌组织中的表达及其临床意义。方法：应用免疫组织化学方法（SP法）检测p27基因在62例官颈癌组织和20例正常官颈组织中的表达。结果：62例宫颈癌组织中,p27的阳性表达率为38．7％（24／62）;p27的阳性表达与宫颈病灶大小、临床分期、细胞分化程度、转移及预后有密切关系（P〈0．01或〈0．05）;与宫颈癌的病理类型及年龄无关（P〉0．05）。结论：p27基因的表达与宫颈癌的生长、浸润及淋巴结转移有关,其表达水平的高低可作为判断宫颈癌预后的重要指标之一。     

Anti-Inflammatory Components of the Starfish Astropecten polyacanthus

Inflammation is important in biomedical research, because it plays a key role in inflammatory diseases including rheumatoid arthritis and other forms of arthritis, diabetes, heart disease, irritable bowel syndrome, Alzheimer’s disease, Parkinson’s disease, allergies, asthma, and even cancer. In the present study, we describe the inhibitory effect of crude extracts and steroids isolated from the starfish Astropecten polyacanthus on pro-inflammatory cytokine (Interleukin-12 (IL-12) p40, interleukin-6 (IL-6), and tumor necrosis factor α (TNF-α)) production in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs). Among those tested, compounds 5 and 7 showed potent inhibitory effects on the production of all three pro-inflammatory cytokines with IC₅₀ values ranging from 1.82 ± 0.11 to 7.00 ± 0.16 μM. Potent inhibitory activities were also observed for compound 1 on the production of IL-12 p40 and IL-6 with values of 3.96 ± 0.12 and 4.07 ± 0.13 μM, respectively, and for compounds 3 and 4 on the production of IL-12 p40 with values of 6.55 ± 0.18 and 5.06 ± 0.16 μM, respectively. Moreover, compounds 2 (IC₅₀ = 34.86 ± 0.31 μM) and 6 (IC₅₀ = 79.05 ± 2.05 μM) exhibited moderate inhibitory effects on the production of IL-12 p40, whereas compounds 3 (IC₅₀ = 22.80 ± 0.21 μM) and 4 (IC₅₀ = 16.73 ± 0.25 μM) moderately inhibited the production of TNF-α and IL-6, respectively.     

秦岭北麓关中平原一次温度异常变化分析

通过对2012年12月4日秦岭北麓关中平原部分地区逐小时气温变化分析,发现12月4日关中平原部分地区出现了一次气温日变化异常过程,西安城区4日上午中度强度的雾霾天气抑制了其局部大气的升温幅度,是4日白天西安城区气温升幅缓慢的原因;西北路冷空气到达关中前下沉增温及秦岭地形阻挡引起的大气压缩增温效应是19:00～21:00关中部分地区出现温度异常升高的主要原因;与周边区县相比,西安城区城市热岛效果显著。