高原农作物豌豆抗氧化和脂质过氧化研究

1材料与方法 1．1材料供试豌豆产于西宁互助,海拔3600m;供试小鼠体重18-22g,雌雄对半,由青海动物养殖场提供。     

Effects of Monoclonal Antibody Against Porcine 40-kDa Adipocyte-Specific Membrane Protein on Endocrine Secretion in Pigs

The present study was to investigate the effect of monoclonal antibody against porcine 40-kDa adipocyte-specific membrane protein on endocrine secretion in pigs, in order to provide the evidence for application of this antibody to reduce excessive fat deposition in pig production. 40 Landrace×Saba pigs were randomly divided into 8 groups： 2 control groups were given saline with 10 mL, respectively, and the 6 treatment groups were given monoclonal antibody against porcine 40-kDa adipocyte-specific membrane protein with 0.1, 0.5, and 1.0 mg ·kg^-1 body weight at 15 or 60 kg body weight, respectively, all treatments were performed by intraperitoneal injection. The results showed that this monoclonal antibody could significantly reduce serum insulin level and increase levels of serum growth hormone （GH）, insulin-like growth factor-1（IGF-1）, triiodothyronine （T3）, and tetraiodothyronine （T4） either at 15 or 60 kg body weight injection. However, more marked effect was observed at 15 kg body weight treatment. Moreover, the dose-dependent effect of this monoclonal antibody on endocrine secretion was also observed. This result revealed that this monoclonal antibody increased secretion of hormones regulating fat lysis and reduced secretion of hormones regulating fat synthesis, suggests the reduction of porcine excessive fat deposition by this monoclonal antibody was carried out through affecting hormones regulating fat metabolism.     

柠檬酸铜对肉鸡生产性能、血脂指标和组织胆固醇含量的影响

【目的】探讨柠檬酸铜对肉鸡生产性能、血脂指标和组织胆固醇含量的影响。【方法】采用单因子设计,将420只7日龄AA肉鸡随机分为7组,每组设3个重复,每个重复20只,公母各半。Ⅰ(对照)～Ⅶ组日粮分别添加0,50,100,150,200,250和300mg/kg柠檬酸铜,49日龄空腹称体质量,计算采食量、日增质量和料肉比。每组取6只鸡,采集血样、肝脏和胸腿肌,测定血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、极低密度脂蛋白(VLDL-C)和高密度脂蛋白(HDL-C)及肝脏、胸腿肌胆固醇含量。【结果】与Ⅰ组(对照)相比,Ⅳ～Ⅶ组肉鸡日增质量及日采食量均显著增加(P<0.05),料肉比显著降低(P<0.05);血清TC、TG、LDL-C、VLDL-C显著降低(P<0.05),HDL-C显著升高(P<0.05);胸腿肌、肝脏中的胆固醇含量显著降低(P<0.05);Ⅰ、Ⅱ、Ⅲ及Ⅳ、Ⅴ、Ⅵ、Ⅶ组间,以上指标差异均不显著(P>0.05)。【结论】日粮中添加150～300mg/kg柠檬酸铜,具有提高肉鸡生产性能、降低肉鸡血脂和组织胆固醇含量的效果。     

低温弱光对茄子幼苗某些生理指标的影响

研究了茄子幼苗在低温弱光（10℃/5℃ 昼/夜,光强60和120μmol/）胁迫和恢复各7d后的部分生理指标的变化。结果表明,低温弱光胁迫后茄子幼苗的净光合速率（Pn）,气孔导度(Gs)和叶绿素(Chl.)含量显著降低;光补偿点(LCP)、光饱和点(LSP)、光饱和时的Pn、表观量子效率(AQY)降低;CO2补偿点（CCP）升高,CO2饱和点（CSP）、CO2饱和时的Pn、光合能力（A350）、CO2羧化效率（CE）降低;POD和CAT活性明显下降;Pro.和MDA含量有不同程度的升高;均以低温下较强光照时（120μmol/）的变化幅度较大;恢复7d后除MDA含量基本恢复到对照水平外,其它各项指标仍然不能恢复到对照水平。试验条件已使茄子幼苗发生了膜脂过氧化作用,并对茄子幼苗叶片光合机构的结构和活性造成了不可恢复性的伤害。     

禽大肠杆菌O2、O78及其耐药、融合菌株的外膜蛋白免疫研究

为了研究大肠杆菌O2、O78及其耐药、融合菌株外膜蛋白的免疫原性,用Sarkosyl处理和超速离心技术相结合的方法分别提取禽大肠杆菌强毒株O2、O78,耐药弱毒株O2(Nor^rChl^s)、O78(Not^sChl^r)及其融合双价弱毒株O2.78(Nor^rCll^r)等5个菌株的外膜蛋白(OMPs),经SDS-PAGE,结果均出现1条相同的相对分子质量在31000-43000之间的主要OMPs条带,表明5个菌株存在相同的OMPs成分,将3个耐药菌株的OMPs分别免疫小白鼠和鸡,结果均产生对O2、O78良好的同源及交叉免疫保护效果,用各耐药菌株的OMPs做包被抗原,ELISA方法都能交叉检测3个耐药菌株OMPs免疫鸡血清中IgG水平,且各IgG的消长曲线基本一致。     

芍药切花瓶插期衰老进程及膜脂过氧化研究

以6个芍药品种‘春晓’、‘山河红’、‘奇花争露’、‘紫凤朝阳’、‘菱花晨浴’、‘蓝海碧波’的切花为材料,瓶插观察其外部形态特征变化,测其膜透性、MDA含量、 超氧阴离子生成速率及SOD、CAT活性的变化,研究其衰老进程、膜脂过氧化与瓶插寿命的关系。结果表明：‘春晓’、‘菱花晨浴’、‘奇花争露’能达到最大开放程度,盛开期为3 d,瓶插寿命分别为8 d、7 d和7 d;‘山河红’、‘紫凤朝阳’、‘蓝海碧波’盛开期为2 d,瓶插寿命均为6 d。瓶插过程中,膜透性、MDA含量、 超氧阴离子生成速率持续上升, SOD、CAT活性先上升后下降。膜脂过氧化造成的细胞膜损伤是启动芍药切花衰老的生理原因之一。     

低温胁迫对扁桃枝条细胞膜系统和渗透调节物质的影响

为了进一步了解6个引进扁桃品种在莎车地区的抗寒性,在冬季的绝对低温下,以本地品种纸皮为对照,对引进的6个扁桃品种,测定其低温胁迫下不同方向枝条的细胞膜透性、MDA含量、可溶性糖含量等生理生化指标的变化.同时结合大田的冻害调查情况分析,以比较引进扁桃不同品种的抗寒性.结果表明:低温胁迫下同品种树体的西面和北面受冻害程度大于东面和南面,不同扁桃品种间的耐寒性存在差异:Sonora＞Mission＞Tompson＞Butte＞Nepulusultera＞Nonpareil.且Nonpareil与本地品种纸皮的抗寒性未表现出显著性差异.从而了解扁桃在低温胁迫下与细胞膜系统、渗透调节物质之间的关系.     

纳滤膜在植物叶子、果实及动物体液、软骨、内脏的活性物质提取中的应用

简述了纳滤膜技术应用于天然活性物质提取的优点。从发明专利角度综述了纳滤膜提取植物的果实、叶子及动物的体液、软骨、内脏中活性成分的国内应用。展望了纳滤技术在该领域应用的发展前景。第2期刘超锋纳滤膜在植物叶子、果实及动物体液、软骨、内脏的活性物质提取中的应用     

CANALOGRAPHIC EVALUATION OF THE EXTERNAL EAR CANAL IN DOGS

Canalography was performed to measure the diameter of the horizontal ear canal and to assess the clinical relevance of the measurements in identifying abnormalities of the ear canal. The diameter of the proximal and distal end of the annular cartilage in the horizontal ear canal was measured using canalography in 222 ears. The tympanic membrane could not be visualized with otoscopic examination in 70 ears even after ear cleansing. These canals were classified as being stenotic. The diameter of the proximal annular cartilage in the stenotic canals ranged from 0.8 to 3.1 mm (mean = 2.6 +/- 0.8 mm). The proximal annular cartilage was consistently smaller in diameter than the distal annular cartilage. The ratio between the diameter of the proximal and distal annular cartilage varied between dogs. In stenotic ear canals (70 ears) the ratio was less than 0.65. Total ear canal ablations and histopathological analysis were performed in 70 ear canals classified as stenotic after canalography. Hyperplasia of the epidermal layer was found in 56 stenotic canals. Stenosis of the canal due to otitis externa was found in the other 14 canals. Three canals with severe otitis externa were also diagnosed with canalography as having a ruptured tympanic membrane. The results suggest that canalography can be used to measure the diameter of the horizontal ear canal and to assess whether or not the tympanic membrane is ruptured prior to conservative or surgical therapy.     

过瘤胃烟酸和胆碱对围产期奶牛泌乳性能和肝脂质代谢的影响

旨在研究日粮中添加过瘤胃保护烟酸(rumen-protected niacin,RPN)和过瘤胃保护胆碱(rumen-protected choline,RPC)对围产期奶牛泌乳性能和肝脂质代谢的影响,为缓解奶牛脂肪肝提供理论依据.本试验选用24头健康、胎次相近的中国荷斯坦围产期奶牛,根据2×2试验设计分为4组,每组6...