The fate of fluroxypyr in the soil in an oil palm agroecosystem

A study on the fate of fluroxypyr in Malaysian agricultural soils was carried out under natural conditions in an oil palm plantation. Three experimental plots in the oil palm estate were selected and three types of treatments were administered, namely fluroxypyr at the recommended dosage, double the recommended rate, and the untreated control. Residues of fluroxypyr in the soil, water, and oil palm leaf were monitored at regular intervals up to 90 days after treatment (DAT). The recovery of fluroxypyr in the water and soil samples ranged from 91–102% when fortified at 1–25 p.p.b. and 91–98% when fortified at 4–50 p.p.b. The recovery from the oil palm leaf ranged from 76–114% when the oil palm leaf sample was fortified at 0.05–1.0 p.p.m. The results showed that fluroxypyr leached down to 50 cm depth at 1 DAT when the plots were treated with either dosage. However, fluroxypyr was not detected in the soil residue at 14 and 21 DAT. Fluroxypyr was not detected in the oil palm leaf samples irrespective of the rates of herbicide application or sampling intervals. The residue of fluroxypyr was detected in water from a nearby stream up to 5 DAT at levels ranging from 0.06–0.21 µg L⁻¹. This result suggests that fluroxypyr persists for a short period in the soil and water ecosystems, but not in the oil palm leaf.     

Detection and characterization of phytoplasmas in diseased stone fruits and pear by PCR-RFLP analysis in Turkey

During the late summer-early autumn of 2002, surveys were carried out in Turkey to determine the presence of phytoplasma diseases in fruit trees. Phytoplasmas were detected and characterized by PCR-RFLP analysis and TEM technique in stone fruit and pear trees in the eastern Mediterranean region of the country. Six out of 24 samples, including almond, apricot, peach, pear and plum, gave positive results in PCR assays. RFLP analysis usingSspI andBsaAI enzymes of PCR products obtained with primer pair f01/r01 enabled identification of the phytoplasmas involved in the diseases. Stone fruit trees, including a local apricot variety (‘Sakıt’) and a pear sample, were found to be infected with European stone fruit yellows (ESFY, 16SrX-B) and pear decline (PD, 16SrX-C) phytoplasmas, respectively. This is the first report in Turkey of PD phytoplasma infecting pear and of ESFY phytoplasma infecting almond, apricot, myrobalan plum and peach; ESFY phytoplasma infecting Japanese plum was previously reported. http://www.phytoparasitica.org posting July 21, 2005.     

苹果采前落果与内源激素的关系

为了探讨苹果采前落果与内源激素之间的关系,在采前8周间定期测定了不同苹果品种的果柄、果台和离层形成部位组织中IAA、ABA含量;在采收前20d内定期测定了离层部位组织中细胞壁分解酶(Cellulase)的活性;在收获期测定了果实乙烯的发生量。结果表明:不同品种果柄、果台和离层部位组织中IAA和ABA含量变化有差异,但它们变化的总趋势相似,都是随着果实成熟IAA含量下降,而ABA含量上升;不同品种的成熟果实中乙烯发生量有很大差异,以落果多的品种显著大于落果少的品种;采前落果重的品种离层部位组织中细胞壁分解酶的活性在果实成熟期急剧增加。由于果实进入成熟阶段后,IAA含量下降,ABA含量升高,ABA/IAA之间的相对平衡被打破,高ABA/IAA以及高乙烯会刺激离层组织中细胞壁分解酶的活性增高,进而促进离层形成,这可能是导致落果发生的原因。     

葡萄子油及原花青素研究与开发利用

综述了葡萄子油、葡萄子原花青素等天然产物的提取与保健作用的研究进展,原花青素的生物活性及药理研究的现状,葡萄子油成分分析方法和葡萄子,提取物中原花青素含量测定的方法;介绍了用超临界CO2萃取法制取高质量的葡萄子油和应用夹带剂提取葡萄子中的原花青素的方法。分析了葡萄子的综合开发的应用前景。     

套袋黄冠梨黑点病与钙素营养和果实衰老的关系

为探明套袋黄冠梨果实黑点病发病原因,从采后生理、矿质营养水平、酚类物质酶促褐变和果实衰老等方面对套袋黄冠梨果实黑点病进行了研究。结果表明:套袋果呼吸强度显著高于不套袋果;套袋果Ca含量仅为不套袋果的49%-63%,果皮钙素含量未套袋果>套袋健康果>套袋病果,套袋病果与不套袋果果皮N/Ca比值分别为9.8和6.1,K/Ca比值分别为17.6和8.8;果皮和果心酚类物质含量及多酚氧化酶活性(PPO)呈套袋病果>套袋健康果>未套袋果的趋势;套袋病果果皮丙二醛(MDA)含量及电导率显著高于未套袋果;果皮钙素含量与其PPO活性、多酚及丙二醛含量、电导率呈高度负相关。初步分析黄冠梨果实黑点病病因为缺钙性生理衰老。     

改良热硼酸法高效提取苹果果实RNA

苹果果实、尤其是成熟期的果实中多糖和其他次生物质含量高,难以提取RNA。对此,在原热硼酸法的基础上进行了改进,通过添加提取辅助剂并根据辅助剂特性调整提取步骤,高效、稳定地从苹果果实、尤其是后期果实中提取到了高质量的RNA。所得RNA的A260/A230大于2.0,A260/A280为1.8-2.1,并且RNA产量高,其中前期果实RNA产率在13.40μg/g以上,后期果实RNA产率在7.02μg/g以上,完全可以满足RT-PCR、cDNA-AFLP等分子生物学实验的要求。     

Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress

Summary A substantial amount of seed is left in the fields before and during harvest of oilseed rape. Although this crop exhibits little or no primary dormancy, the absence of certain environmental cues that promote germination of imbibed seeds induces secondary dormancy. The work reported investigated the extent to which environmental stress conditions, including osmotic stress, low oxygen stress and anaerobiosis, induce secondary dormancy in oilseed rape, and examined the variation in development of secondary dormancy between and within genotypes. Osmotic stress was most effective in inducing dormancy. Anaerobic treatment produced very few dormant seeds, as did an atmosphere low in oxygen and high in nitrogen. The development of secondary dormancy under osmotic stress varied considerably between and within genotypes. Dormancy ranged from almost zero to about 60% for winter genotypes and about 85% for spring types. Within genotypes, variations occurred between seed lots and years of harvest. Temperature variations affected the percentage of dormant seeds. More dormant seeds were likely to be produced with incubation under water stress at 20 °C than at 12 °C. In winter genotypes, fewer dormant seeds were produced when incubation temperature and germination test temperatures differed. Thus, incubating at 20 °C and 12 °C, followed by germination tests at 20 °C and 12 °C, respectively, produced most dormant seeds. Also, in the winter genotypes, the potential development of secondary dormancy was positively correlated with the pattern and speed of germination of untreated seeds.     

Movement of Xanthomonas campestris pv. vitians in the stems of lettuce and seed contamination

Xanthomonas campestris pv. vitians , the causal agent of bacterial leaf spot of lettuce (BLS), can be seedborne, but the mechanism by which the bacteria contaminates and/or infects lettuce seed is not known. In this study, the capacity of X. campestris pv. vitians to enter and translocate within the vascular system of lettuce plants was examined. The stems of 8- to 11-week-old lettuce plants were stab-inoculated, and movement of X. campestris pv. vitians was monitored at various intervals. At 4, 8, 12 and 16 h post-inoculation (hpi), X. campestris pv. vitians was recovered from 2 to 10 cm above (depending on stem length) and 2 cm below the inoculation site. Xanthomonas campestris pv. vitians was also recovered from surface-disinfested stem sections of spray-inoculated plants. Together, these results are consistent with X. campestris pv. vitians invading and moving systemically within the vascular system of lettuce plants. To investigate the mechanism of seed contamination, lettuce plants at the vegetative stage of growth were spray-inoculated with X. campestris pv. vitians and allowed to develop BLS. Seed collected from these plants had a 2% incidence of X. campestris pv. vitians external colonization, but no bacteria were recovered from within the seed.     

不同粘度矿物油助剂对除草剂活性的影响

室内及温室内测定了10种不同粘度矿物油助剂对除草剂防除苘麻和稗草药效的影响及对玉米苗期的安全性。结果表明,10种矿物油助剂均不同程度地增加了氰草津和烟嘧磺隆防除两种杂草的活性。其中粘度为46 mm2/s (40℃)的矿物油乳剂(MOC46)对供试除草剂药效的增强作用最为明显,其次为粘度32 mm2/s (40℃)的矿物油乳剂。10种助剂对氰草津药效的增强作用大于对烟嘧磺隆。MOC46对除草剂活性的增加由高到低依次为:莠去津、氰草津＞莠去津+氰草津、莠去津+烟嘧磺隆、氰草津+烟嘧磺隆＞烟嘧磺隆;对玉米苗期安全性依次为:莠去津、烟嘧磺隆、莠去津+烟嘧磺隆＞莠去津+氰草津、氰草津+烟嘧磺隆＞氰草津。氰草津与MOC46混用对玉米苗期药害较重。     

离体条件下水稻种衣剂对串珠镰刀菌生长及形态毒理影响

 检测了黑龙江水稻主产区4个当地主栽品种的种子内部镰刀菌寄藏情况,测定了20%克福甲和20%克多甲种衣剂对种子带菌消毒处理效果及对水稻串珠镰刀菌(Fusarium moniliforme)的抑菌作用和联合毒力,并借助扫描电镜观察了上述2种混配种衣剂对串珠镰刀菌的形态毒理影响。结果表明,种子内部镰刀菌的分离频率高达56.7%~96.0%,其中串珠镰刀菌的分离频率为32.6%~48.2%,2种种衣剂对带菌种子具有显著的消毒处理效果。20%克福甲和20%克多甲种衣剂对镰刀菌F.moniliforme的毒力指数分别为457.11和802.04,增效倍数分别为6.53和0.13。20%克多甲种衣剂(多菌灵:甲基立枯磷为5:5,W/W)对串珠镰刀菌的抑菌作用优于20%克福甲种衣剂(福美双:甲基立枯磷为8:6,W/W),增效作用低于20%克福甲种衣剂。电镜观察表明,种衣剂低浓度至高浓度处理下均可引起串珠镰刀菌菌丝不同程度的异常生长,表现为主菌丝局部膨大或形成菌丝束,菌丝顶端异常膨大、缢缩或形成穗状和花絮状分枝。