福建省几种典型红壤性水稻土稀土含量的研究——Ⅱ.稀土与土壤成分的相关分析

对供试土壤的稀土总量(Σ REE),轻稀土含量(Σ LREE)和重稀土含量(Σ HREE)与土壤颗粒组成、有机质含量、腐殖质组成及不同形态氧化铁含量之间的相关关系进行了统计分析.结果表明:(1)ΣREE、Σ LREE 与土壤粘粒,有机质、腐殖质及组成,Fe_t,Fe_d,Fe_(d_o),Fe_(t-d)之间均呈极显著相关;(2)Σ HREE 与上述物质的相关均不显著.     

4种松树抗寒性的比较

通过人工冰冻处理和电导法测定了美国黄松、奥地利黑松、花旗松和油松4个针叶树种在不同低温条件下的电解质渗出率,然后用Logistic方程进行配合,求算组织的半致死温度。结果表明,花旗松耐受低温能力强,组织半致死温度-20.6℃,美国黄松耐低温较差,半致死温度-10.7℃,奥地利黑松与油松接近,居于二者之间。花旗松针叶组织的束缚水含量及其与自由水比值最大,抗性强;美国黄松自由水含量高,束缚水与自由水比值小,抗性弱。综合比较结果,花旗松抗寒性最强,美国黄松最弱,奥地利黑松和油松居中。     

光强、温度和N/P比对坛紫菜叶状体单性生殖发生的影响

为探究培养条件对坛紫菜叶状体生长和单性生殖发生的影响,研究了不同光强、温度和N/P比下坛紫菜叶状体的生长及单性生殖发生的特点。结果显示,当光强为10～40μmol/(m2·s)时,藻体生长缓慢、成熟晚、单性生殖发生晚;光强增至60～80μmol/(m2·s)时,藻体生长加快,成熟和单性生殖发生同时提前。温度为17～20°C时,藻体生长慢、成熟晚、单性生殖发生推迟;23°C时藻体生长最快;而29°C组的藻体虽然生长受到抑制,但藻体成熟和单性生殖发生得到促进。N/P比为16∶1处理组的叶状体比其他处理组(1∶1、4∶1、32∶1和64∶1)均生长变快,N/P比为64∶1时叶状体生长最慢,但易成熟、易发生单性生殖。因此,在高光强、高温和高N/P比下,藻体成熟和单性生殖发生均提早,说明这些因素对坛紫菜叶状体单性生殖的发生有重要影响。     

影响木薯叶片蛋白质双向电泳图谱的因素分析

以木薯华南205为试材,采用苯酚抽提法提取叶片蛋白质,并通过双向电泳技术研究影响木薯叶片蛋白电泳分析图谱的主要因素。结果表明：pH 4～7的IPG胶条可提高特定范围蛋白质点的分辨率;上样量200 μg可得到较好的双向电泳图谱。本研究不仅为进一步研究木薯叶片蛋白电泳分析提供技术手段,同时也为分析木薯块根、茎和繁殖器官的蛋白质提供参考。     

Effects of the herbicide LASSO MTX (alachlor 42% W/V) on biometric parameters and liver biomarkers in the common carp (Cyprinus carpio)

The aim of the study was to evaluate the effect of subchronic exposure to the herbicide LASSO MTX (alachlor 42% W/V) on biometric parameters and important liver biomarkers in the common carp (Cyprinus carpio). One year old fish were exposed for 28 days to LASSO MTX added to the tank water at concentrations of 240 and 2400 μg L⁻¹. The exposure did not affect fish biometric parameters. Glutathione-S-tranferase (GST) activity in liver (hepatopancreas) remained unchanged in exposed fish when compared to controls. However, significant induction of total cytochrome P 450 (CYP 450), ethoxyresorufin-O-deethylase (EROD) activity and elevated glutathione (GSH) in liver of exposed fish were detected.     

樱桃番茄嫁接砧木筛选研究

以格格樱桃番茄作接穗,农优野茄、托鲁巴姆、金钻砧木和砧木1号作砧木,进行嫁接和嫁接苗栽培试验.试验结果表明.4种砧木与樱桃番茄嫁接亲和性由强到弱依次为:砧木1号、金钻砧木、农优野茄和托鲁巴姆;嫁接苗青枯病发病率与自根苗差异均达到极显著水平,嫁接苗产量比自根苗增产15.9%～48.7%,在抗青枯病和产量方面表现都比较好的砧木为农优野茄和砧木1号.     

Over-expression of P21 attenuates cisplatin-induced HK-2 cells injury

AIM:To investigate the effect of P21 on cisplatin-induced renal tubular epithelial cells injury.METHODS:The expression of P21 at mRNA and protein levels in cisplatin treated human renal tubular epithelial cells (HK-2) cells was determined by RT-qPCR and Western blot. Over-expression of P21in the HK-2 cells was induced by the transfection of pcDNA3-P21. The cell viability and cell apoptosis were detected by CCK-8 assay and flow cytometry, respectively. Furthermore, the protein expression of kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), caspase-3, glucose-regulated protein 78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP), and phosphorylation level of eucaryotic translation initiation factor 2α (eIF2α) and protein kinase R-like endoplasmic reticulum kinase (PERK) were detected by Western blot.RESULTS:Cisplatin increased the mRNA and protein levels of P21 in a time-and concentration-dependent manner in the HK-2 cells. Over-expression of P21 inhibited cisplatin-induced cell apoptosis, and down-regulated the expression of KIM-1 and NGAL. Furthermore, Over-expression of P21 decreased the protein levels of GRP78, p-PERK, p-eIF2α, CHOP and cleaved caspase-3.CONCLUSION:Over-expression of P21 attenuates cisplatin-induced HK-2 cells injury, and the mechanism may be related to the modulation of endoplasmic reticulum stress pathway and inhibition of cell apoptosis.     

Dietary available phosphorus requirement for juvenile gibel carp (Carassius auratus gibelio var. CASIII)

A 57‐day growth experiment was conducted with juvenile gibel carp (13.48 ± 0.10 g) in a flow‐through system to study the effect of dietary phosphorus on growth performance, body composition, nutrition utilization, phosphorus loading and enzymes activities. Seven semipurifed diets were formulated to contain 0.07 (the basal), 2.27, 5.32, 8.10, 12.06, 15.24 and 19.48 g available phosphorus/kg diet. The results showed that specific growth rate, body length and feed efficiency significantly increased in the fish fed diets containing 0.07 to 15.24 g available P/kg diet (p < .05). Ash and P content increased in fish fed diets containing 0.07–12.06 P g/kg (p < .05) and then levelled off, while moisture, crude protein and lipid had no significant difference (p > .05). The protein retention efficiency increased in the fish fed with diets 0.07–5.32 g/kg P (p < .05) and then reached a plateau. The P content in faeces was higher in fish fed diets containing 15.24 and 19.48 g available P/kg. Total P concentration in tank water increased in fish fed 0.07–12.06 g available P per kg diet (p < .05). The plasma P was higher in the fish fed with 15.24 g available P/kg diet (p < .05), triglycerides was lower in the fish fed diet containing 15.24 and 19.48 g available P/kg (p < .05), no significant differences were observed in plasma Ca, plasma glucose and calcitonin (p > .05). Based on SGR, whole body P content and FE, dietary available P requirement for juvenile gibel carp were 13.37, 13.97 g/kg and 15.06 respectively.     

鲤血清转铁蛋白纯化及其与铁离子的结合特性

用硫酸铵分级沉淀法及制备性ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）法,获得含有鲤（Ｃｙｐｒｉｎｕｓｃａｒｐｉｏ）血清转铁蛋白（Ｔｆ）的ＰＡＧ条带,同时除去ＳＤＳ及考马斯亮蓝,分离纯化了转铁蛋白,并做铁离子结合试验及光谱分析。结果表明,转铁蛋白结合铁离子后的特异吸收峰为４６７ｎｍ,说明,去ＳＤＳ后转铁蛋白仍有很强的铁离子结合能力,用普通ＳＤＳ－ＰＡＧＥ法测得鲤血清Ｔｆ分子量约为７４０００Ｄａｌ     

卡拉库尔羊妊娠母羊铜、锌、铁、锰的含量测定

为了探索微量元素对妊娠母羊营养状况的影响,选取体重相近、年龄和胎次相同的卡拉库尔羊妊娠母羊和空怀母羊各6只,清晨采取颈静脉血样和臀部毛样,利用原子吸收光谱法对铜、锌、铁、锰4种微量元素含量进行了测定(国标法)。结果表明:血清中,铜的含量在妊娠母羊与空怀母羊之间差异显著(P<0.05);全血中,铁、铜、锰含量在妊娠母羊与空怀母羊之间差异显著(P<0.05);羊毛中,铜、锰含量在妊娠母羊与空怀母羊之间差异显著(P<0.05)。