高能混合粒子场诱变小麦的细胞学效应研究

利用北京正负电子对撞机直线加速器E2束流打靶产生高能混合粒子场,以0、109、145、1952、84和560Gy的剂量处理两个冬小麦品种ZY9和ZH7,并与相同剂量的60Coγ射线相比较,研究其细胞学效应。试验结果表明,混合粒子场辐照小麦种子可抑制根尖细胞有丝分裂,诱发染色体出现单微核、双微核、多微核、环状染色体、落后染色体、游离染色体、染色体断片等多种畸变类型。混合粒子场与γ射线诱发的微核畸变率和染色体畸变率均存在明显的剂量效应,但混合粒子场的损伤效应明显高于γ射线。混合粒子场可诱发高频率的环状染色体和染色体断片,显示出混合粒子场处理小麦具有比γ射线处理更高的相对生物学效应。     

不同类型微型蒸发器测定土壤蒸发的田间试验研究

利用微型蒸发器(Micro-Lysimeter,简称ML)测定土壤蒸发是一种简单、有效的方法,在中国西北旱区有很大的利用价值,但目前还没有统一的ML设计标准,所以寻求适合西北旱区使用的ML类型具有重要的现实意义。该文利用不同直径、不同长度、不同材料以及不同封底情况的ML在西北旱区进行了砂土和壤土的蒸发测量试验。研究结果表明：测得的土壤蒸发量随ML直径的增大而减小,不同直径ML测得的蒸发量在0.05的水平下有显著差异,测量砂土蒸发采用直径为15 cm的ML效果较好,壤土采用10 cm的较为理想。直径较小的情况下,ML的长度对蒸发量的影响显著,直径较大时则不显著,ML的长度以20c m为宜。ML的制作材料和是否封底对蒸发测量也有显著影响,在西北旱区,采用PVC不封底的ML较好。     

Soil sulphur status following long-term annual application of animal manure and mineral fertilizers

 In agricultural systems with low S inputs, crops rely on the release of S from organic forms in the soil. In the Askov long-term experiments, started in 1894 on both sandy and loamy soils, soil S status following long-term application of animal manure and mineral fertilizers was investigated in the growing season of 1995. In a field trial with oil-seed rape (Brassica napus, L.) soil analysis, leaf tissue analysis, yield and S removal in plant material was used to characterize differences in availability of soil S. One half of all plots received 63 kg S ha^–1 as gypsum. Long-term fertilization with animal manure or NPK fertilizer increased the content of soil organic C in both soils and of organic S in the sandy soil compared with unfertilized plots. Although dry matter yields were unaffected, the S uptake in harvested crop parts increased considerably after S application. The amounts of N and S in harvested seeds and straw were closely related, but the N : S ratio decreased when S was applied. Soil and plant analyses both indicated that critical levels of S concentrations were reached, and that S application was capable of raising S concentrations well above the critical level. Because no additional mineralization from residual organic S took place, it was concluded that the residual S effect from long-term annual applications of animal manure or mineral fertilizers did not significantly increase the level of soil S available for crops with a short growing season, such as oil-seed rape. Received: 9 January 1998     

甜玉米填闲减缓菜田土壤硝酸盐淋溶的研究

为提高菜田氮肥的利用率,降低氮肥对环境的污染风险。在中德合作项目东北旺试验田（土壤类型为潮土）,三年（1999～2001）9季蔬菜长期传统施氮灌水与推荐施氮灌水处理的地力基础上,于高温多雨的夏季选择甜玉米作为填闲作物,以休闲处理作对照,研究甜玉米在土壤硝态氮淋溶关键期对土壤残留硝态氮素分布及对后茬蔬菜产量的影响。研究结果表明甜玉米生长迅速、生物量大、吸收氮素能力强,吸氮量可达205.6～246.1 kg/hm²;与休闲处理相比,种植甜玉米能有效的吸收0～60 cm土壤中残留氮素,实现了土壤残留氮素的再利用。0～180 cm剖面中土壤硝态氮的残留量都有不同程度的降低,有效阻抑了氮素向土壤深层的淋洗。甜玉米也获得了较高的经济产量,穗净鲜重达9.2～10.2 t/hm²。后茬作物菠菜收获后未被利用的氮素大部分残留在土壤浅层;甜玉米处理与休闲处理相比未显著影响后季菠菜的生长,产量达18.4～20.7 t/hm²。该研究表明：甜玉米是较为理想的填闲作物。     

Genomewide association study reveals novel quantitative trait loci associated with resistance towards Septoria tritici blotch in North European winter wheat

Fungal diseases are a major constraint for wheat production. Effective disease resistance is essential for ensuring a high production quality and yield. One of the most severe fungal diseases of wheat is Septoria tritici blotch (STB), which influences wheat production across the world. In this study, genomewide association mapping was used to identify new chromosomal regions on the wheat genome conferring effective resistance towards STB. A winter wheat population of 164 North European varieties and breeding lines was genotyped with 15K single nucleotide polymorphism (SNP) wheat array. The varieties were evaluated for STB in field trials at three locations in Denmark and across 3 years. The association analysis revealed four quantitative trait loci, on chromosomes 1B, 2A, 5D and 7A, highly associated with STB resistance. By comparing varieties containing several quantitative trait loci (QTL) with varieties containing none of the found QTL, a significant difference was found in the mean disease score. This indicates that an effective resistance can be obtained by pyramiding several QTL.     

圆柏枝叶对小鼠自发行为及生理生化指标的影响

选择城市绿化重要树种圆柏为研究对象,探讨北美圆柏枝叶对小鼠自发活动及其生理生化指标的影响及挥发性物质与环境间的关系。采用旷场分析法,将入组的30只昆明小鼠分成3组:对照组、低剂量组和高剂量组,低剂量组和高剂量组分别对应地暴露于210 g和420 g圆柏枝叶中,每天4 h,连续处理6 d,观察小鼠各行为指标,结合医学手段分析小鼠相应的生理生化指标。结果表明,小鼠的中央格运动路程、总路程及中央格运动时间都呈现一定的剂量反应;体质量呈稳定增长趋势;生理指标正常,生化指标有部分超出正常范围。经低浓度枝叶挥发物处理的小鼠兴奋性增强,探究和认知能力增强,且其食欲没有受到影响,但是引起部分生化指标的异常,其作用机制还需进一步的探索。研究结果为圆柏作为生态保健树用于城市森林人居环境建设绿化配置提供科学依据。     

基于玉米种植的生长育肥猪粪污农田承载力

本研究旨在以生长育肥猪和玉米种植农田为模型,准确评定生长育肥猪粪肥养分的产生量和不同施肥量下玉米的生物产量及养分需求量,以此为基础建立基于粪肥还田利用的玉米种植农田粪肥承载系数。试验选取8头13周龄胎次和体重（（33.2±3.5）kg）相近的"杜×长×大"生长育肥猪进行饲养试验,分别于16和25周龄各进行一次消化试验,测定其粪、尿产量及粪肥氮、磷产生量。玉米种植试验设6个处理组,对照组施用化肥,负对照组不施肥,4个试验组分别按玉米种植全期氮标准需要量的100%、130%、160%和磷标准需要量的100%施用猪粪有机肥,分别在乳熟后期和完熟期测定玉米种植的氮、磷需求量,与猪粪肥氮、磷产生量拟合,测算粪肥农田承载系数。结果表明,按130%氮需求量施用猪粪有机肥,一季玉米种植乳熟后期和完熟期收获,对氮、磷的需求量分别为122.1、53.0 kg·hm^-2和190.2、62.8 kg·hm^-2,按130%氮施肥量下乳熟后期和完熟期收获的玉米农田承载系数,以N为基础为61.1头·hm^-2和95.2头·hm^-2,以P为基础为90.5头·hm^-2和107.3头·hm^-2,而且施用猪粪有机肥还可增加玉米生物产量,一定程度上增加玉米中氮、磷含量。因此,分别基于玉米对氮和磷的需求,单季玉米种植每公顷土地生长育肥猪的承载参数分别为95.2头和107.3头。     

冬小麦秸秆还田对夏玉米生长发育及产量的影响

【目的】探讨不同品种冬小麦秸秆、秸秆覆盖量和覆盖方式对夏玉米生长发育和产量的影响。【方法】于2012年在陕西关中一年两熟典型地区,采用随机区组设计,选用小偃22和小偃216 2个冬小麦品种秸秆,设秸秆混入耕层、秸秆带状覆盖、秸秆全面覆盖3种秸秆还田方式,以及4 500,7 500和10 500kg/hm2 3个秸秆还田量,以秸秆不还田为对照,于夏玉米幼苗期和发育中期测定株高、茎粗、次生根数、叶片颜色、单株干质量,并于成熟期测定株高、穗位高及产量构成因素,研究各处理对夏玉米生长发育及产量的影响。【结果】2012年夏玉米播种后由于较长时期干旱少雨,冬小麦秸秆覆盖的保墒作用大于其化感作用,各秸秆还田处理夏玉米生长发育及产量总体优于对照。在2个小麦品种秸秆中,以小偃216秸秆对夏玉米诸性状的表现较好;在3种秸秆还田量中,以7 500kg/hm2秸秆还田量较为适宜;3种秸秆还田方式中,则以秸秆带状覆盖方式对夏玉米生长发育影响较小;在此组合下,夏玉米幼苗和植株生长健壮,成熟期产量最高。【结论】对夏玉米进行秸秆覆盖时,以冬小麦秸秆覆盖量为7 500kg/hm2的带状覆盖方式最好。     

蔬菜农药残留问题分析及控制对策

介绍了当前蔬菜农药残留的主要来源及类型,探讨了蔬菜中农药残留对人畜和环境的危害,分析了蔬菜农药残留超标的根本原因,并提出了降低农药残留的对策,以提高农产品质量安全水平。     

新型具有力感知的柔性果蔬采摘机械手研究

研发了基于设施农业的果蔬采摘机械手,包括具有3个旋转关节自由度的采摘机械臂及具有力反馈功能的三指果蔬采摘末端执行器,并对其控制系统进行了开发。通过采摘模拟试验,验证了其作业性能、采摘效果。试验结果表明:采摘机械手各关节与控制系统配合良好,运行稳定;采摘末端执行器手指控制灵活,传感器响应灵敏,能够实现稳定抓取并不损伤果实,具有推广价值。