促进麝香增产的新途径研究

通过研究,发现外激素对麝的生香过程有调节控制作用,利用这个原理,定期给雄麝以外激素刺激,结果雄麝的年产香量明显提高,获得了显著的经济效益。此外,依据麝释放化学信息的原理,提出了回收麝香的可行性措施,为扩大麝香资源,提高麝香产量开辟了新途径。     

朝鲜鹌鹑遗传多样性微卫星标记分析

选用鹌鹑上的12个微卫星位点,对随机选取朝鲜鹌鹑的40个个体进行多态性检测,共检测到55个等位基因,每个座位平均等位基因数为4.583个。该群体平均多态信息含量和平均杂合度分别为0.6945和0.7111,表明朝鲜鹌鹑属多态性较丰富的群体。     

财务信息对上市公司高层变动的影响分析

上市公司高层变动受多种因素的影响,经营业绩是其中一个重要因素,能有效反映公司经营绩效的财务信息,对高层变动的影响也是不容忽视的.通过对深市样本公司的高层变动与会计信息的实证分析,进一步证明了不同会计信息对高层变动的影响程度及二者的关系,从而为投资者和管理者及其他财务数据使用者提供有效的参考依据.     

广西动物防疫物资信息管理系统的构建与应用

为实现广西动物防疫物资的信息化管理,适时掌握广西动物防疫物资的流转状况,广西壮族自治区动物疫病预防控制中心开发了“广西壮族自治区动物防疫物资信息管理系统”。该系统为B/S架构,采用JSP技术和MySQL数据库平台。该系统建立后,广西各级动物疫病预防控制机构均可通过互联网登录,及时存储和统计动物防疫物资信息数据,全程监控和实时了解物资流转情况。该系统为广西动物防疫物资的规范化管理提供了重要的技术支持,也为全区重大动物疫病防控提供了坚强的后勤保障。     

How do visual and auditory cues influence calf stress reactions at weaning?

We investigated the effects of visual and auditory information related to cow and calf stress responses at weaning. Three weaning conditions were established with physical separation only (PS), physical and visual separation (VS), and physical, visual and auditory separation (VAS). Stress responses such as vocalization and self‐grooming, and changes of three maintenance behaviors (lying, walking and grazing) were recorded for experimental cows and calves in the respective conditions. Comparison of the data revealed that the cow responses differed slightly among groups. However, the peak frequency of vocalization was higher in the PS calves than in those of either VS or VAS. Self‐grooming frequency was significantly different in calves and was high in the order of VAS, VS and PS calves. Furthermore, the percentage of time spent lying down was greater, and that of walking was less, in VAS calves than in either VS or PS calves. These results demonstrate that different information at weaning might strongly affect the stress responses of calves but not those of cows. Relative impacts of different types sensory information on stressors must be considered to plan animal‐friendly weaning.     

河南省陆地植被净第一性生产力估算及其时空分布

基于地理信息系统和卫星遥感技术,利用地面气象数据和MODIS数据,同时考虑河南省自身实际地理和植被覆盖情况,在对最大光利用率和水分胁迫系数的获取进行改进的基础上,利用CASA模型对河南省植被净第一性生产力（NPP）进行了估算,并分析了其时空分布。结果表明,2008年河南省NPP生产量为34.87 Mt C,NPP空间分布和植被类型有密切关系,森林植被最大,然后依次是灌丛、草地、农田等。12、1和2月NPP最小,植物基本停止生长,植被的NPP只占全年的1.38％;5、7和8月的NPP最大,占全年总量的56.84％。与Miami、Montreal、Chinkugo模型相比,本研究结果和Miami模型结果最为接近,植被类型NPP均值排序和Chinkugo模型相似;与实测数据对比结果显示,模拟值接近于实测值,说明模型适用于河南省NPP的模拟。     

基于RNA-Seq技术的苜蓿根蘖性状发生相关下调基因

为了从分子水平上分析苜蓿(Medicago sativa)根蘖性状的发生机制,对根蘖型苜蓿的根蘖与非根蘖根部进行RNA-seq转录组文库构建,并分析差异表达基因及其功能。分别提取品系"BL-101"的根蘖型苜蓿根蘖与非根蘖部位总RNA,利用磁珠法分离m RNA后通过PCR扩增构建RNA-Seq转录组文库,并对差异表达基因进行分析,得到差异表达基因15 978条,本研究重点分析下调基因,通过生物过程分类,发现与根蘖性状发生有关的表达下调的基因为5个,经生物学信息分析确定,其中2个属于Lon蛋白酶家族,其余分别属于植物肌动蛋白酶家族,硫氧还蛋白和脂氧合酶,其与植物抗逆性相关,且与植物激素ABA的调控有关。研究结果可以为探讨苜蓿根蘖性状发生的分子机制提供理论依据。     

Evaluation the Clinitek status automated dipstick analysis device for semiquantitative testing of canine urine

The aim of this prospective study was to evaluate the Clinitek status™ analyser using Multistix10SG™/Microalbustix™ dipsticks (all: Siemens Dx) for canine urine (n = 101) compared to reference methods: visual reading (Combur9 dipstick, Roche), refractometry, microscopy, and quantitative protein/creatinine analysis (Pentra400, AxonLab). The automated analyses were done twice and visual tests were performed by two examiners.An excellent to good concordance was demonstrated between the first/second analysis with the Multistix10SG and the Combur9 dipstick, respectively with Cohen’s κ-values ranging from 0.776 to 1.000. Agreement between both dipsticks was good for glucose (κ = 0.753), blood (κ = 0.793), protein (κ = 0.788), and moderate for bilirubin (κ = 0.431) and ketones (κ = 0.540). In 6/101 specimens, false positive ketone reactions were obtained with the Multistix10SG™. Multistix10SG™ could not be used for determination of pyuria or specific gravity. Semiquantitative/quantitative protein results correlated well (ρ = 0.90) and creatinine measurements moderately (ρ = 0.76). Due to automated data transmission to the laboratory information system, the Clinitek status™ is of advantage in veterinary laboratories/clinics.     

2012年湖南省奶业的发展状况与前景展望

为促进湖南省奶业发展,本文对2012年湖南省奶业信息进行了监测.监测结果表明,2012年湖南省奶牛存栏数比2011年下降59.8％.生鲜乳价格持续走低,而奶牛饲料价格普遍上涨.生鲜乳质量安全状况一般,防治费用较高,部分乳制品加工企业拖欠奶款严重,使奶牛养殖面临困境.     

五指山猪小群体遗传学检测

本研究利用26个微卫星基因座对中国热带农业科学院热带作物品种资源研究所保种场的五指山猪小群体58个样本进行了遗传学检测,用非变性(中性)聚丙烯酰胺凝胶电泳检测微卫星的PCR扩增产物,统计了群体平均基因纯合率、等位基因数,利用等位基因频率计算出各群体的平均遗传杂合度(H)、多态信息含量(PIC)。统计结果:全群平均基因纯合率为66.5%;平均多态信息含量、平均观测杂合度为、平均期望杂合度分别为0.807、0.338、0.835;每个位点平均等位基因数为11.92,此结果说明该群体虽然近交程度较高,但仍具有丰富的遗传多样性,遗传变异较大。