羊肚菌冷冻干燥加工技术研究

通过实验,研究确定了了冻干羊肚菌的共晶点和共融点,得到了最佳冻干曲线,通过对冻干羊肚菌和传统风干羊肚菌的理化指标及感官指标的比较,证实了冷冻干燥羊肚菌的加工工艺的优越性。     

超高效液相色谱-串联质谱法检测棉花和土壤中呋虫胺残留

在QuEChERS方法基础上,建立了棉花和土壤中呋虫胺残留的超高效液相色谱-串联质谱(UPLC-MS/MS)快速检测分析方法。方法选用乙腈为提取剂,N-丙基乙二胺(PSA)和石墨化炭黑(GCB)为净化剂,外标法定量。添加回收试验结果表明,不同添加浓度的呋虫胺(0.01、0.05和0.5mg/kg)在棉花植株、棉籽和土壤中的平均回收率为80.9%~107.5%,变异系数为3.3%~10.3%,定量限(LOQ)分别为4.81、3.41和2.26μg/kg。呋虫胺在棉花植株上的消解动态表明,呋虫胺在河南省和山东省两地棉花植株中的降解半衰期分别为1.9d和1.2d。     

HPLC-MS/MS快速检测水产品中甲基睾丸酮的研究

用甲基叔丁基醚提取水产样品中的甲基睾丸酮,提取物经C18粉和正己烷净化后,通过电喷雾离子源电离多反应监测(MRM)对甲基睾丸酮作定性和定量分析。结果表明,甲基睾丸酮在1.0～500.0μg/L浓度范围内线性良好(r>0.999);在4.0～200.0μg/kg添加水平下,回收率为80.4～95.2%,相对标准偏差为4.1%～10.5%,检测限为1.0μg/kg(S/N=5)。     

Antioxidant Efficacy of a Spirulina Liquid Extract on Oxidative Stress Status and Metabolic Disturbances in Subjects with Metabolic Syndrome

Lipid peroxidation is associated with the development of some pathologies, such as cardiovascular diseases. Reduction in oxidative stress by antioxidants, such as Arthrospira (formely Spirulina), helps improving this redox imbalance. The aim of the study was to evaluate the effect of the Arthrospira liquid extract “Spirulysat^®” on oxidative markers—in particular, oxidized LDL (oxLDL)/total LDL cholesterol—and isoprostanes and to investigate its impact on lipid and glucose metabolism in the metabolic syndrome subject. A controlled, randomised, double-blind design was conducted in 40 subjects aged 18 to 65 years with metabolic syndrome after a daily intake of Spirulysat^® or placebo for twelve weeks. Blood and urinary samples were collected at three visits (V1, V2, V3) in the two groups for parameters determination. Although the Spirulysat^® group showed a decrease at all visits of the oxLDL/total cholesterol ratio, there was no significant difference compared to the placebo (p = 0.36). The urinary isoprostanes concentration in the Spirulysat^® group was reduced (p = 0.014) at V3. Plasma triglycerides decreased at V3 (p = 0.003) and HDL-cholesterol increased (p = 0.031) at all visits with Spirulysat^®. In conclusion, Spirulysat^® did not change the oxidized LDL (oxLDL)/LDL ratio but decreased the urinary isoprostanes, plasma triglycerides and increased HDL cholesterol, suggesting a beneficial effect on metabolic syndrome.     

1-O-Alkylglycerol Ethers from the Marine Sponge Guitarra abbotti and Their Cytotoxic Activity

The cytotoxicity-bioassay-guided fractionation of the ethanol extract from the marine sponge Guitarra abbotti, whose 1-O-alkyl-sn-glycerol ethers (AGEs) have not been investigated so far, led to the isolation of a complex lipid fraction containing, along with previously known compounds, six new lipids of the AGE type. The composition of the AGE fraction as well as the structures of 6 new and 22 previously known compounds were established using ¹H and ¹³C NMR, GC/MS, and chemical conversion methods. The new AGEs were identified as: 1-O-(Z-docos-15-enyl)-sn-glycerol (1), 1-O-(Z-docos-17-enyl)-sn-glycerol (2), 1-O-(Z-tricos-15-enyl)-sn-glycerol (3), 1-O-(Z-tricos-16-enyl)-sn-glycerol (4), 1-O-(Z-tricos-17-enyl)-sn-glycerol (5), and 1-O-(Z-tetracos-15-enyl)-sn-glycerol (6). The isolated AGEs show weak cytotoxic activity in THP-1, HL-60, HeLa, DLD-1, SNU C4, SK-MEL-28, and MDA-MB-231 human cancer cells. A further cytotoxicity analysis in JB6 P⁺ Cl41 cells bearing mutated MAP kinase genes revealed that ERK2 and JNK1 play a cytoprotective role in the cellular response to the AGE-induced cytotoxic effects.     

CnAβ基因对大鼠RBL-2H3细胞脱颗粒的影响

过敏是人和养殖动物常见的疾病,而且发生的频率呈现逐渐增加的趋势.为了深入了解过敏反应的机制,本研究利用CRISPR/Cas9基因编辑技术,构建钙调磷酸酶A beta(calcineurin A beta,CnAβ)基因敲除的大鼠RBL-2H3细胞株,并探讨CnAβ基因对RBL-2H3细胞生长及脱颗粒的影响.选取大鼠Cn...     

副溶血性弧菌PCR检测方法的建立

为了建立适于基层单位应用的针对副溶血性弧菌检测的特异PCR方法,检测副溶血性弧菌的基因序列.针对该菌的属特异性基因t1基因进行引物设计,扩增片段大小为449 bp.运用该PCR法可特异性的从副溶血性弧菌ATCC17802标准株中扩增出目的基因片段,与GenBank上发表的序列同源性为100%.而经常污染海产品的溶藻弧菌,嗜水气单胞茵标准株J-1,铜绿假单胞茵标准株ATCC27853结果均为阴性.该PCR法最低检出菌体DNA量为10-2ng以及最低检出菌数为5.7 × 103 CFU/mL.对临床上送检的35份样品进行菌体DNA PCR方法检测并同时与国标GB/T4789.7-2003中使用的检测方法进行比对,两种检测方法的结果完全符合,与国标中使用的检测方法相比可大大缩短鉴定时间.因此该PCR法能快速鉴定当前副溶血性弧菌流行群,有利于流行病学溯源等研究.     

牦牛病毒性腹泻病PCR诊断方法的建立

采用阻断ELISA方法对来自天峻县的327份牦牛血清进行了牛BVDV特异性抗体的检测,共检出231份血清阳性,血清阳性率为70.64%。其中:在135份野牦牛血清中检出110份阳性血清,阳性率为81.48%;在192份家牦牛血清中检出阳性121份,阳性率为63.02%。从231份ELISA检测阳性血清中随机抽取了20份,采用RT-PCR方法进行检测,结果共检出18份样品为BVDV/MV RT-PCR阳性,阴性血清未扩增出E0基因,RT-PCR方法检测结果与ELISA方法符合率为90%。     

基于B/S模式无纸化考试系统的设计与实现

本文给出了基于B/S结构的无纸化考试系统的设计模型,并从设计思路、开发环境、系统功能以及几个关键技术等方面进行了阐述,最后给出有待进一步研究的问题.     

牦牛奶渣乳酸菌对虉草青贮品质的影响

为评价牦牛奶渣乳酸茵对藕草青贮饲料发酵品质和化学成分的影响,试验设置对照组（CK）和3个不同乳酸茵含量处理组（Nzl：0．1ml·kg-1FM;NZ2：0．3ml·kg-1FM;NZ3：0．5ml·kg—FM）,青贮60d后检测发酵品质和化学成分。试验表明：不同添加剂比例对藕草青贮饲料影响不同;NZ1和NZ3处理能够显著降低祷草青贮饲料的pH（P〈0．05）,具有较高的乳酸和乙酸含量（P〈0．05）,NZ3处理的中性、酸性洗涤纤维含量显著降低（P〈0．05）,说明牦牛奶渣乳酸茵可以改蒿草青贮饲料的发酵品质;随着添加浓度的增加赫草青贮饲料的可溶性碳水化合物和粗蛋白具有显著增加趋势,中、酸性洗涤纤维具有降低趋势,说明牦牛奶渣乳酸菌能够改籍草青贮饲料的营养价值;牦牛奶渣乳酸茵的最适添加量为0．5ml·kg-1FM。