高压水射流破碎岩石数值模拟

针对高压水射流破碎岩石的特性，结合多物质流固耦合算法与ALE(任意拉格朗日欧拉)算法，利用显示动力学有限元程序对该复杂过程进行数值模拟，得出岩石破碎过程中的流体与固体的相互作用及岩石的破碎动态扩展过程并对连续射流冲击与脉冲射流冲击对岩石的切割效果进行对比。     

Variation among stallions in sperm quality after single layer centrifugation

Although single layer centrifugation (SLC) selects robust spermatozoa from stallion semen, the effect of individual variation has not been studied in detail. The objective of this study was to determine the variation among stallions in the effects of SLC on sperm quality during cooled storage for up to 48 hr. Semen samples from seven stallions (18 ejaculates) were split, with one portion being used for SLC and the other serving as a control (CON). Sperm quality (kinematics, reactive oxygen species (ROS) production, membrane integrity (MI) and chromatin integrity) were analysed at 0, 24 and 48 hr using computer-assisted sperm analysis and flow cytometry. Sperm quality was better in SLC than in CON at all timepoints, especially chromatin integrity and MI (p < .0001 for both), and some categories of ROS production (e.g. proportion of live hydrogen peroxide negative spermatozoa, p < .0001), but the degree of improvement varied among stallions and type of ROS (p < .05–p < .0001). Total and progressive motility were also better in SLC samples than in CON at 24 and 48 hr (p < .0001), although the effect on sperm kinematics varied. The interaction of treatment, time and stallion was not significant. In conclusion, sperm quality was better in SLC samples than in CON, although there was considerable individual variation among stallions. The improvement in sperm quality, particularly in chromatin integrity, was clearly beneficial, and therefore the use of this technique would be warranted for all stallion semen samples.     

磷酸、膦酸酯类农药的质谱裂解规律研究

在对23种磷酸、膦酸酯类农药及其氧化物的质谱数据进行归纳整理的基础上,明确了这类农药质谱裂解规律。结果表明:电子轰击离子化条件下,磷酸、膦酸酯类农药分子极易裂解,且分子离子峰较弱。发现C_2H_6O_3P~+(m/z109)是其最具代表性的特征碎片离子。磷酸酯类农药裂解规律是二甲氧基磷酸酯P-0键易发生a断裂,生成m/z 109特征碎片离子。二乙氧基磷酸酯易脱去中性碎片C_2H_4,形成m/z81的特征离子。R'含有烯烃结构,易产生m/z127的特征碎片离子。膦酸酯类农药裂解规律与磷酸酯类似,特征性弱,膦键容易发生a断裂。选定了磷酸、膦酸酯类农药在质谱分析中常见的碎片离子,为农药残留筛查、确证分析提供参考。     

Body size, niche breadth, and ecologically scaled responses to habitat fragmentation: mammalian predators in an agricultural landscape

The ability to make a priori assessments of a species' response to fragmentation, based on its distribution in the landscape, would serve as a valuable conservation and management tool. During 1997-1999, we monitored 717 scent stations to examine seasonal use of forest patches, corridors, and crop fields by coyotes (Canis latrans), domestic cats (Felis catus), foxes (Vulpes vulpes and Urocyon cinereoargenteus), raccoons (Procyon lotor), striped skunks (Mephitis mephitis), opossums (Didelphis virginiana), and long-tailed weasels (Mustela frenata). For each species we developed landscape-based ecologically scaled landscape indices (ELSI), and we modeled species spatial distribution across three spatial scales (landscape-level, element-level, and local habitat-level). Our results suggest that these predators view landscape fragmentation at different spatial scales and demonstrate strong interspecific differences in their response to elements of the landscape. All species except coyotes and domestic cats avoided agricultural fields. In general, predator species that were more mobile (i.e. high ESLI for landscape connectivity; coyotes) were characterized by landscape- and element-based logistic models. In contrast, models including local habitat features generally were most appropriate for less mobile or more stenophagous predators (e.g. long-tailed weasels). Our analysis extends the application of the ESLI concept to species assemblages that do not appear to function as metapopulations, and it highlights the importance of examining spatial scale and species-specific responses to habitat fragmentation. We discuss the relevance of these findings for defining ecological landscapes, understanding predator-prey interactions at multiple spatial scales, and conserving predator and prey populations in fragmented landscapes.     

Impacts of land fragmentation on input use,crop yield and production efficiency in the mountains of Nepal

Amidst the growing concern about agricultural underdevelopment in developing countries, this study addresses the causes and trends of land fragmentation. It analyses the impact of land fragmentation on input use efficiency, crop yield and production efficiency, especially with reference to a mountain district in Nepal. Necessary information was obtained from discussions with groups and individual land users and a questionnaire survey covering 184 households representing different degrees of land fragmentation. The analyses focused on two staple crops, namely, maize and paddy. The analysis revealed an increasing trend in the number of land parcels and a decreasing trend in the size of land parcels, primarily due to the heredity tradition of equal division of land among the inheritors. Other factors such as land purchase have also contributed to land fragmentation. Yield analysis revealed that small parcels are more productive than large parcels, because of higher applications of inputs. Consistent with this, small parcels also appeared to have a higher production efficiency than large parcels—considering both benefit and cost of purchased inputs—indicating a positive impact of land fragmentation on farmers' income. However, an analysis considering also the cost of inputs produced on the farm revealed an opposite trend, that is, on large land parcels, production is more efficient than on small parcels. In view of the need to also recover the cost of the inputs produced on‐farm, it is concluded that land fragmentation—leading to small plots—has a negative impact on production efficiency, thereby constraining agricultural development. Copyright © 2007 John Wiley & Sons, Ltd.     

基于核密度估计的京津冀地区耕地破碎化分析

为了深入分析京津冀地区耕地破碎化和人工干扰程度,以空间网格为基本单元,采用核密度函数法对耕地进行密度计算,继而采用自然断点法进行密度分区,采用景观格局指数法,对耕地的空间分布特征、破碎化程度进行定量分析,最后采用耕地破碎度指数和人工干扰指数对比分析提出耕地整治的方向。结果表明:耕地核密度变化范围为0~95.08点/km2,85%以上的耕地集中分布在区域中部和东南部的中密度区到高密度区,耕地面积随着密度降低而减少;耕地斑块密度指数、斑块边界密度指数、面积加权分维数和耕地斑块聚集度在不同密度区的变化情况均反映出破碎化程度随着耕地密度减小而增大;耕地破碎度指数和人工干扰度指数均随着耕地密度降低而升高,人工干扰是该区域耕地破碎化程度加剧的主要因素。采用核密度估计法能合理地反映耕地聚集程度和破碎化程度,可为京津冀地区协同发展、耕地整治和土地利用优化布局提供理论支撑。     

泥胡菜水提物的体外抗菌作用试验

采用试管预试法和圆形滤纸层析法对泥胡菜的化学成分进行分析,结果表明,泥胡菜含有生物碱、有机酸、蛋白质、多肽、氨基酸、酚性成分、鞣质、糖类等化学成分;用纸片法和试管法对泥胡菜水提取液进行体外抗菌作用试验,结果表明,泥胡菜水提取液对金黄色葡萄球菌、巴氏杆菌、链球菌、沙门菌、大肠埃希菌都有较强的抗菌作用.抑菌圈直径分别为19.66 mm±0.53 mm、13.45 mm±0.21 mm、15.84 mm±1.23 mm、12.78 mm±0.34 mm、12.43 mm±0.52 mm;泥胡菜水提取液对金黄色葡萄球菌、巴氏杆菌、链球菌、沙门菌、大肠埃希菌的最低抑菌浓度(MIC)分别为0.063、0.125、0.25、0.125、0.25 g/mL.最低杀菌浓度(MBC)分别为0.063、0.25、0.5、0.125、0.25 g/mL.     

Relationship between reservoir size and genetic differentiation of the stream caddisfly Stenopsyche marmorata

Reservoirs have the potential to block the gene flow of stream macroinvertebrates along a channel, which takes place via larval drift and adult flight, resulting in genetic differentiation above and below the reservoirs. Gene flow across the reservoirs may be strongly obstructed if the area of standing water is larger. Using random amplified polymorphic DNA markers, we investigated the genetic structure of Stenopsyche marmorata (Stenopsychidae: Trichoptera) populations found above and below the reservoirs, and the reference stream of six reservoirs with small to large water surface area ranging from 0.12 km² to 6.00 km². As a result, the two largest reservoirs with a standing water area larger than 3.27 km² showed significant differences in pairwise θ between fragmented and reference streams, whereas the other four reservoirs with a standing water area smaller than 1.64 km² showed insignificant differences. The genetic differentiations in the two largest reservoirs did not result in the reduction of genetic diversities in the fragments. Based on the significant correlation between relative population size and mean expected heterozygosity, we concluded that local genetic diversities were constrained in smaller populations due to the effect of genetic drift.     

耕地细碎化对耕地利用效率的影响——基于不同经营规模农户的实证分析

耕地细碎化是我国现代农业发展面临的突出问题,解决耕地细碎化问题是提高耕地利用效率的关键。基于农户耕地经营规模划分农户类型,利用武汉城市圈部分区县农户调查数据,采用DEA-Tobit模型分析了地块平均面积表征的耕地细碎化对耕地利用效率的影响。研究结果表明:1)小规模农户耕地经营面积平均为0.194 hm~2,利用效率为0.360;中规模农户耕地经营面积平均为0.492 hm~2,利用效率为0.446;大规模农户耕地经营面积平均为1.179 hm~2,利用效率为0.497;耕地利用效率与农户耕地经营规模呈正相关关系。2)耕地细碎化对农户耕地利用效率具有显著的影响,但对不同经营规模农户的影响存在显著差异;耕地细碎化对小规模农户的耕地利用效率具有正向作用,而对中、大规模农户的耕地利用效率具有负向作用;相对于小规模农户而言,细碎化对大规模农户的耕地利用效率的影响较大。因此,提高耕地利用效率,应鼓励耕地流转,为农户规模化经营耕地创造条件;在大力推进农业规模化经营的过程中,应将耕地整治与耕地流转有机结合起来,降低细碎化程度,以提高耕地利用效率。     

自然保护区布局监测和生境破碎化监测——以海南铜鼓岭自然保护区为例

建立自然保护区是保护生物多样性的主要方法和手段。我国的自然保护区建设发展得很快,但是仍存在许多困难和问题,当务之急是建立一套完整的自然保护区管理体系、监测体系,组织自然保护区开展资源、生态等方面的监测工作。应用GAP对自然保护区的布局和现状进行分析表明,目前尚未得到有效保护的优先生态系统有27类。并以海南铜鼓岭自然保护区为例,利用RS和GIS技术对自然保护区的生境破碎化进行了监测研究。结果表明研究区生境破碎化程度增加,致使斑块内部物种向外迁移,生物多样性降低。