卵泡液中总蛋白含量与卵泡直径的关系

通过检测不同直径卵泡的卵泡液中总蛋白含量的变化,从而进一步阐明卵泡液中蛋白成分在卵泡发育过程中的作用。将直径为3~5、5~8、8~10mm的卵泡和囊肿卵泡(>21mm)分为4组(n=10),分别抽取卵泡液。通过TCA/丙酮方法进行样品处理;根据Bradford法检测原理,利用分光光度计对其进行蛋白定量研究。结果表明:3~5mm为4440μg/mL,5~8mm为976μg/mL,8~10mm为686μg/mL,囊肿卵泡(>21mm)为537.2μg/mL。经SPSS13.0软件分析,3~5mm卵泡液中蛋白平均浓度高于其他组(P<0.01);其他3组间差异均不显著(P>0.05);囊肿卵泡中蛋白含量最低。由此可得出,卵泡液中总蛋白浓度随着卵泡直径的增加而降低,表明卵泡液中的蛋白含量与卵泡发育的成熟度呈负相关。而囊肿卵泡的发生很可能和蛋白含量降低有关,为卵泡囊肿发病机制的研究提供新的理论参考。     

厌氧悬浮颗粒污泥床反应器流态和运行状态相关关系研究

第三代高效厌氧反应器是在第二代反应器基础上,主要对水力流态进行较大改进而产生的新型反应器,该类型反应器不仅具有第二代反应器生物量高的特点,同时又具有良好的水力混合特性,大大提高了反应器的处理效率。本文选择厌氧悬浮床反应器作为研究对象,考察了反应器在运行过程中运行状态与流态之间的关系,建立高效厌氧反应器水力流态模型,利用水力学流态模型的测定结果,讨论不同条件下反应器的短流、死区和膨胀率等影响。     

The influence of anticoagulants on the measurement of total protein concentration in equine peritoneal fluid

The aim of this study was to evaluate the influence of two commonly used anticoagulants (K3EDTA and lithium heparin) on refractometric and spectrophotometric measurement of total protein (TP) concentration in equine peritoneal fluid samples. The influence of a commercial solution of K3EDTA, a solution of K3EDTA in distilled water and lithium heparin on the refractometric and spectrophotometric (biuret) quantification of TP content in peritoneal fluid samples was assessed. Total protein concentration measured by refractometry was consistently overestimated in samples with commercial K3EDTA. The solution of K3EDTA in distilled water only caused TP overestimation at high K3EDTA concentrations (>5 micromol/ml). By contrast, lithium heparin did not influence the refractometric values of TP. Neither anticoagulant modified TP values when measured by the biuret method. In conclusion, the use of K3EDTA as anticoagulant may result in a significant overestimation of TP values of peritoneal fluid samples measured by refractometry.     

Neospora caninum associated with septic peritonitis in an adult dog

A 7-year-old, male neutered Rhodesian Ridgeback dog was referred to the University of California-Davis Veterinary Medical Teaching Hospital with a 4-month history of peritonitis and episodic abdominal discomfort, lethargy, and weakness. Marked abdominal distension with a prominent fluid wave was noted on physical examination. Cytologic analysis of the abdominal fluid indicated a septic exudate with mixed bacteria and many protozoal zoites. Differentials for the identity of the protozoal zoites included Toxoplasma gondii, Sarcocystis neurona, and Neospora caninum. Indirect latex agglutination antigen testing, standard indirect fluorescent antibody testing, and PCR analysis were performed to identify the zoites. The dog's serum antibody titer for N caninum tachyzoites was 1:20,480, known polysera to N caninum reacted against zoites in the abdominal fluid, and PCR analysis of the abdominal fluid was positive for the presence of a known gene of N caninum. Based on the morphologic, immunologic, and molecular findings, the zoites were identified as N caninum. It remains unclear how the tachyzoites gained access to the peritoneal cavity. To the authors' knowledge, there are no reports of free N caninum in abdominal fluid of any species.     

Cerebrospinal fluid analysis and magnetic resonance imaging in the diagnosis of neurologic disease in dogs: a retrospective study

BACKGROUND: Diagnosis of central nervous system (CNS) abnormalities in dogs can be challenging antemortem. Historically, cerebrospinal fluid (CSF) analysis has been used for routine diagnostic evaluation of animals with suspected neurologic disease; however, with increasing availability of magnetic resonance (MR) imaging, the need for concurrent CSF analysis may be questioned. OBJECTIVE: The purpose of this study was to retrospectively assess and compare the diagnostic information contributed from MR imaging and CSF analysis in a population of dogs presenting with neurologic disease. METHODS: Results of concurrent MR imaging and CSF analysis were evaluated in dogs presented for neurologic diseases. Based on clinical diagnosis, the sensitivity of CSF analysis and MR imaging for detecting a nervous system abnormality was calculated. Dogs with diagnoses confirmed by other diagnostic modalities were also evaluated separately. RESULTS: A total of 256 dogs were included in the study. For clinical diagnoses in which abnormalities were expected, MR imaging abnormalities were found in 89% and CSF abnormalities in 75% of dogs; CSF abnormalities were more common than MR imaging abnormalities only in inflammatory CNS disease. The majority of CSF abnormalities were nonspecific; an etiologic diagnosis was determined in only 2% of CSF samples. MR imaging excelled in detecting structural disorders, revealing 98% of vertebral abnormalities. In confirmed cases (n = 55), 76% of MR images and 9% of CSF samples were diagnostic. When intervertebral disk disease (IVDD) and vertebral malformation were excluded from analysis (n = 16 remaining), 25% of MR images and 6% of CSF cytology results were highly indicative of the confirmed diagnoses; CSF titer results provided the diagnosis in 25% of these cases. CONCLUSION: CSF analysis may not be necessary when MR findings of IVDD or vertebral malformation/instability are obvious; however, when the cause of neurologic disorder is uncertain, concurrent MR imaging and CSF analysis provides the greatest assistance in establishing a clinical diagnosis.     

CO2超临界萃取法提取苦瓜籽油及其GC-MS分析

一种野生品种苦瓜的籽用索氏提取法提取苦瓜油,测得含油率为38.0％;苦瓜籽仁粉末在CO2超临界状态下,通过正交试验法对萃取压力、温度、时间和CO2流量等因素进行筛选,确定苦瓜籽油萃取工艺参数为萃取压力37 MPa、萃取温度40℃、CO2流量40 L/h、萃取时间为2h时苦瓜籽油得率为97.2％;超临界状态下萃取的苦瓜籽油经GC-MS分析结果表明,苦瓜籽油多种不饱和脂肪酸含量达79.60％,高含量多不饱和脂肪酸的存在使其具有生物活性产品的开发价值.