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121.
122.
Histochemical localization of proteolytic activities in the dorsal epidermis of Japanese eel was demonstrated by fluorescent microscopy utilizing 4-methoxy-2-naphthylamide (4M$\beta$NA) derivatives as substrates and 5-nitrosalicylaldehyde as a trapping agent. Carbobenzoxy-L-phenylalanyl-L-arginyl-4M$\beta$NA (Cbz-Phe-Arg-4M$\beta$NA) and Cbz-Arg-Arg-4M$\beta$NA were used for direct detection of cathepsins L and B activities, respectively, in fresh frozen sections and unfixed cells of the eel epidermis. The fluorescing areas, where Cbz-Phe-Arg-4M$\beta$NA was hydrolyzed by cathepsin L, were shown in mucus secretory cells and club cells and broadly around skin surface. The fluorescing areas due to Cbz-Arg-Arg-4M$\beta$NA hydrolysis by cathepsin B were localized similarly in these tissues. The fluorescing intensity for both catheptic activities in mucus secretory cells was higher than that in club cells, where small fluorescing granules were distributed. These results indicate that eel cathepsins L and B are stored in epidermal secretory cells at different levels and probably serve as defense factors before or after secretion by these cells. Abbreviations: Cbz – carbobenzoxy; 4M$\beta$NA – 4-methoxy-2-naphthylamide; NSA – 5-nitrosalicylaldehyde. 相似文献
123.
Lynne van Herwerden William J. Aspden Stephen J. Newman Graham G. Pegg Leica Briskey William Sinclair 《Fisheries Research》2009,100(2):148-155
Lethrinus miniatus and Lutjanus sebae are important commercial and recreational species of reef fish. Within Australian waters the former species is less widespread than the latter and has a discontinuous distribution, whilst the latter is continuously distributed in tropical Australian waters. The demographic attributes of these species (e.g. long life span, low rates of natural mortality) make them vulnerable to over-exploitation. Consequently, conservative harvest strategies including no-take zones for these species have been adopted by fisheries management agencies to control exploitation. Information on the genetic stock structure of these species is important for developing specific management strategies. However, little is known about genetic stock structures within and between east and west Australian populations of these species. The current study used the mitochondrial genome hypervariable region 1 (HVR1) of the control region to examine variation between two sites from both the east and west Australian coasts for each species. HVR1 for L. sebae did not differ genetically either within or between coasts (Fst < 0.018, p > 0.15) at the sites studied, suggesting a panmictic population structure. Similarly, L. miniatus did not differ significantly between sites sampled within coast. However, the west coast HVR1 for L. miniatus east and west coast populations, were discrete (Fst of at least 0.92, p < 0.0001). The degree of genetic sub-division between east and west coast populations indicates that they should be managed as discrete stocks. Further, when considering both species, the lower genetic (both haplotype and nucleotide) diversity in three of the four sites on the west coast of Australia, indicates that this region is genetically impoverished and neutrality tests suggest that selection is responsible. Consequently, west Australian populations will be less resilient to perturbations (e.g. fishing, climate change) than east Australian populations, which have higher genetic diversity. 相似文献
124.
Marine fisheries target and catch fish both for direct human consumption (DHC) as well as for fishmeal and fish oil, and other products. We derived the fractions used for each for 1950–2010 by fishing country, and thus provide a factual foundation for discussions of the optimal use of fisheries resources. From 1950 to 2010, 27% (~20 million tonnes annually) of globally reconstructed marine fisheries landings were destined for uses other than DHC. Importantly, 90% of fish destined for uses other than DHC are food‐grade or prime food‐grade fish, while fish without a ready market for DHC make up a much smaller proportion. These findings have implications for how we are using fish to feed ourselves or, more appropriately, how we are not using fish to feed ourselves. 相似文献
125.
通过Eviews31软件就1990年到2008年我国国民收入与茶叶内销售量关系进行分析,得出我国国民收入与茶叶内销量存在协整关系,同时运用格兰杰检验得出我国国民收入提高是我国茶叶内销量增加的成因,认为通过预测我国国民收入水平可推算出我国茶叶的内销量,为茶叶产销决策提供依据。 相似文献
126.
应用ANSYS程序建立了几种不同渡槽槽体结构的有限元模型,数值结果表明,采用板壳单元能很好地模拟槽体的剪力滞效应,计算精度满足工程要求。对单跨简支单箱、多箱槽体的剪力滞效应进行了较详细的数值研究,得到了槽体底板与上部翼缘的纵向正应力分布规律,研究了渡槽的截面形式、宽跨比等参数对渡槽剪力滞效应的影响。研究结果对渡槽结构设计具有参考价值。 相似文献
127.
128.
借助于紫外扫描,通过对氯化铝显色法、硝酸铝—亚硝酸钠显色法和直接测定法3种比色方法与高效液相色谱法测定结果的对比和方法评价的研究,确定了直接测定法(测定波长为350 nm)是一种操作简单、结果较准确的方法。以芦丁为标准品,可用于中原牡丹花黄酮含量的快速测定。 相似文献
130.
The attraction ofTrichogramma platneri Nagarkatti (Hymenoptera: Trichogrammatidae) to eggs of two lepidopterous avocado pests,Boarmia sel-enaria (Schiffermüller) (Geometridae) andCryptoblabes gnidiella (Millière) (Phycitidae), was evaluated in olfactometer tests. The mobility of the wasp as measured by a mobility index was
0.6 for males and 1.8 for females; the males had a lower mobility and a lower search capacity index than the females. Frozen
and fresh youngB. selenaria eggs had the greatest attraction forT. platneri, which diminished with increasing age of the eggs. The eggs ofC. gnidiella were also attractive to the parasitoid.
Contribution from the Agricultural Research Organization. No. 2704-E, 1989 series. 相似文献