全文获取类型
收费全文 | 137718篇 |
免费 | 8063篇 |
国内免费 | 10717篇 |
专业分类
林业 | 13226篇 |
农学 | 9128篇 |
基础科学 | 9996篇 |
33631篇 | |
综合类 | 58231篇 |
农作物 | 5621篇 |
水产渔业 | 4063篇 |
畜牧兽医 | 11787篇 |
园艺 | 3420篇 |
植物保护 | 7395篇 |
出版年
2024年 | 980篇 |
2023年 | 2821篇 |
2022年 | 4146篇 |
2021年 | 4677篇 |
2020年 | 4740篇 |
2019年 | 5399篇 |
2018年 | 3590篇 |
2017年 | 5708篇 |
2016年 | 7023篇 |
2015年 | 5509篇 |
2014年 | 7321篇 |
2013年 | 8302篇 |
2012年 | 11469篇 |
2011年 | 10362篇 |
2010年 | 8163篇 |
2009年 | 8219篇 |
2008年 | 7381篇 |
2007年 | 8336篇 |
2006年 | 7004篇 |
2005年 | 5800篇 |
2004年 | 4538篇 |
2003年 | 3818篇 |
2002年 | 3007篇 |
2001年 | 2779篇 |
2000年 | 2380篇 |
1999年 | 1938篇 |
1998年 | 1569篇 |
1997年 | 1433篇 |
1996年 | 1261篇 |
1995年 | 1285篇 |
1994年 | 1146篇 |
1993年 | 920篇 |
1992年 | 755篇 |
1991年 | 702篇 |
1990年 | 543篇 |
1989年 | 479篇 |
1988年 | 312篇 |
1987年 | 252篇 |
1986年 | 126篇 |
1985年 | 64篇 |
1984年 | 49篇 |
1983年 | 30篇 |
1982年 | 26篇 |
1981年 | 31篇 |
1980年 | 23篇 |
1979年 | 16篇 |
1978年 | 14篇 |
1976年 | 9篇 |
1962年 | 13篇 |
1956年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 234 毫秒
981.
文章从学生实践能力和创新能力两个核心能力培养方面进行了理论研究与实践探索。首先简要介绍了专业概况与研究背景,然后对几个核心概念进行了定义与解释,最后结合齐齐哈尔大学的一些做法与经验提出了大类招生下机械电子工程专业学生实践和创新能力培养的建议。 相似文献
982.
983.
984.
985.
To study the effect of weaning stress on dairy buffalo calves,5 healthy calves were chosen and weaned,and the blood samples were collected at 1 day before weaning,and 1,14 and 21 days after weaning to analysis blood physiology and biochemistry indexes and immune function.The results showed that the count of white blood cell,lymphocyte,erythrocyte,monocytes and hematocrit,aspartate aminotransferase activity,blood glucose,cortisol and immunoglobulin G contents of dairy buffalo calves were no significant difference between before and after weaning treatments (P > 0.05).The count of neutrophils at 21 d after weaning were significantly lower than that of 1 d before weaning of dairy buffalo calves (P < 0.05).However,lactate dehydrogenase and alanine aminotransferase activity in weaned treatments were significantly higher than those of animals in the treatment before weaning (P < 0.05).The serum urea nitrogen of dairy buffalo calves at 21 d after weaning was significantly higher than that of 1 d before weaning (P < 0.05) and hemoglobin content at 21 d after weaning was significantly lower than that at 1 day before weaning (P < 0.05).It indicated that the physiological and immunity function of dairy buffalo calves were challenged under weaning stress. 相似文献
986.
The objective of the study was to investigate the effects of supplemental complex water soluble vitamins on production performance, blood biochemical parameters and oxidative resistance of dairy cows in spring and summer.The experiment was designed by 2×2 factorial randomized blocks design.Eighty healthy dairy cows with similar parity and body weight were randomly divided to four groups, with 7 days of adaptation and 70 d experimental phase.The dairy cows in group Ⅲ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in spring.The dairy cows in group Ⅱ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in summer.The results showed that the ADFI and daily milk yield of dairy cows receiving water soluble vitamin in spring were extremely significantly higher (P<0.01);The ADFI of dairy cows receiving water soluble vitamin was significantly higher in summer (P<0.05) and daily milk yield were extremely significantly higher (P<0.01);The butterfat rate was extremely significantly increased and SCC was extremely significantly decreased in spring and summer (P<0.01);Supplementation of water soluble vitamin was also significantly increased LYM (P<0.05).In summer, T-AOC, T-SOD were extremely significantly increased (P<0.01), and TP, CK, LDH, TP, HDL and ALB were extremely significantly decreased (P<0.01) by water soluble vitamin supplementation than that of control group.In conclusion, complex water soluble vitamins could increase the production performance and non-specific immune function, improve the oxidative resistance and relieved the heat stress of the dairy cows. 相似文献
987.
To establish a rapid,sensitive and specific assay for the differential detection of Nipah virus (NiV) and highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV),a duplex Real-time RT-PCR was developed with specific primers and probes targeting to the special sequences of NiV M gene and HP-PRRSV nsp2 gene by optimization of reaction conditions.The performance of the assay was linear ranging from 4.6×101 to 4.6×107 copies/μL for RNA standard control of NiV M (NiV-M-RNA) and from 4.1×101 to 4.1×108 copies/μL for RNA standard control of HP-PRRSV nsp2 (HP-PRRSV-nsp2-RNA),and detection limits of the assay was 46 copies for the NiV-M-RNA and 4.1 copies for the HP-PRRSV-nsp2-RNA,respectively.The coefficients of variation (CVs) of both inter-assay and intra-assay repeatability were less than 2.0%,showing good repeatability.The assay was able to specifically detect NiV and HP-PRRSV simultaneously without cross-reaction with classical swine fever virus (CSFV),porcine epidemic diarrhea virus (PEDV),swine influenza virus (SIV),porcine parvovirus (PPV),pseudorabies virus (PRV) and porcine circovirus type 2 (PCV2).Of the 236 samples from pigs for both NiV and HP-PRRSV detection by the established assay,all the samples were negative for NiV,8 samples were HP-PRRSV positive.In conclusion,this assay offers a useful approach for the differential detection of NiV and HP-PRRSV in clinical specimens from the pigs. 相似文献
988.
为验证APSIM模型对宁夏海原地区草田轮作系统的适用性,基于10年生苜蓿(Medicago sativa)与小麦(Triticum aestivum)、谷子(Oryza sativa)草田轮作试验数据和同期气象资料,运用APSIM模型对系统进行了模拟。通过试错法和文献记载完成了苜蓿、小麦和谷子几个品种的参数本地化。用统计和图形校验方法评价了APSIM模型模拟结果的可靠性和准确性。结果表明,谷子-小麦-小麦(MWW)、小麦-谷子-小麦(WMW)、谷子-谷子-小麦(MMW)、谷子-小麦-谷子(MWM)、小麦-谷子-谷子(WMM)、小麦-小麦-谷子(WWM)6种轮作方式下产量实测值和模拟值的决定系数R2值范围在0.83至0.98之间,D值范围在0.94至0.99之间,表现出了良好的相关性和一致性。土壤含水量实测值和模拟值的决定系数R2值范围在0.52至1之间,D值范围在0.92至0.97之间,相关性和一致性表现良好。表明APSIM模型对宁夏海原地区苜蓿与小麦、谷子轮作具有较好的模拟能力,可以用来模拟分析该地区草田轮作系统生产潜力和土壤水分动态,对该地区气候变化影响下草田轮作的优化管理具有一定指导意义。 相似文献
989.
鸡毒支原体(MG)是对养禽业危害很大的支原体,主要导致禽类慢性呼吸道疾病(CRD),以禽的结膜炎、产蛋率及饲料转换率下降、屠宰率下降等为主要特征。MG可通过垂直和水平传播方式在鸡群中传播,每年给全球家禽产业带来巨大经济损失。随着对MG细胞表面抗原黏附素蛋白(pMGA)和PvpA、GapA的结构与功能研究的深入,K株、TG5株等MG疫苗研究也取得较大进展。由于抗生素的滥用,MG基因中也发生耐药突变,产生了QRDRs等抗药结构,导致MG在耐药性上也出现新的特点。论文主要对国内外MG的疫苗开发、耐药情况和检测技术等进行综述,旨在对家禽MG的综合防控提供借鉴。 相似文献
990.
为探讨不同放牧管理模式下高寒草甸草原土壤养分特征的变化,对青藏高原东缘禁牧(NG)、全生长季休牧(RG)、传统夏季休牧(TG)和连续放牧(CG)4种放牧管理模式下高寒草甸草原土壤有机质、全氮、全磷、全钾、速效氮、速效磷和速效钾含量进行测定和分析。结果表明:(1)NG显著增加了0~30cm土层有机质含量,RG和TG显著增加了0~20cm土层有机质含量;(2)NG,RG和TG显著提高了高寒草甸草原0~30cm土层全氮含量和20~30cm土层速效氮含量,而降低了0~10cm土层的速效氮含量;(3)NG,RG和TG显著提高了高寒草甸草原0~30cm土层全磷和速效磷含量;(4)不同模式放牧对高寒草甸草原土壤全钾含量无显著影响,但总体上,NG,RG和TG高于CG;NG,RG和TG降低了0~10cm土层速效钾含量,而显著增加了10~30cm土层速效钾含量。连续放牧使土壤养分流失严重,从而导致土壤肥力降低,而禁牧和季节性休牧能有效增加青藏高原东缘高寒草甸草原土壤的养分含量,是该区和类似区域草地放牧管理的理想选择。 相似文献