应用均匀设计研究电场刺激及培养因素对甘蓝叶原生质体分裂频率的影响

应用均匀设计法探讨了电场刺激及培养因素对甘蓝叶原生质体分裂频率的影响。结果表明 ,电场方向和强度强烈地影响细胞分裂频率。培养成分对细胞分裂频率均有不同程度的影响。均匀设计能筛选最优的植物原生质体培养条件或培养基。结合“回归 -通径”分析 ,有效地综合评判和研究了各培养成分对原生质体分裂频率的作用     

The inhibition of growth by sub-optimal temperatures and the potential for selection at the cellular level

Summary At present research into the inhibition of plant growth by sub-optimal temperatures is concerned with the examination of a large number of factors. No single aspect of the response to temperature has been found to be of paramount importance. As a consequence selection for low temperature tolerance is complicated.Here a new approach is proposed in which inhibition of cell division is regarded as a limiting process in growth under sub-optimal temperatures, and the role of other processes as the supply of substrate for cell division. This approach may be of use in the examination of the response of plant growth to temperature and could form the basis of a method for screening and selection for cold tolerance using cells in culture.Guestworker Project Group Tomato Physiology.     

Occurrence of 2n gametes in the F1 hybrids of Oriental × Asiatic lilies (Lilium): Relevance to intergenomic recombination and backcrossing

Cytological modes of the origin of 2n gametes were investigated in six different genotypes of F₁ hybrids between Oriental and Asiatic (OA) lilies (Lilium, 2n = 2x = 24). Chromosome pairing between the parental genomes was very low, the average frequency range from 0.3 to 1.2 bivalents per cell among the genotypes. Within a genotype the frequency of bivalents varied from 0 to 6 in some cases. The normally occurring haploid pollen grains were totally sterile. In contrast, in different genotypes, variable percentages of 2n pollen were found and shown to be fertile as estimated from pollen germination. A cytological analysis of Metaphase I and subsequent stages of meiosis using genomic in situ hybridization (GISH) revealed that there was intergenomic recombination between the alien genomes. Following Metaphase I stage, three different types of abnormal cytological events led to the formation of 2n pollen: (i) Post-Metaphase I division (PMI), (ii) Post-Metaphase II division (PMII) and (iii) Asymmetric Cytokinesis of the pollen mother cell followed by chromosome division. All three cytological events led to first division restitution (FDR) gametes. Based on in vitro pollen germination it was proved for two genotypes that 2n pollen was viable only during the first day of anthesis. It was possible to use 2n pollen successfully for backcrossing. Implications of 2n pollen for intergenomic recombination in BC₁ progenies are discussed.     

Reproductive Behavior of Hexaploid/Diploid Wheat Hybrids 1

The bottleneck restricting introgression of useful genes directly from diploid into hexaploid wheats is the low number of BC₁F₁ seeds obtained. In crosses between hexaploid wheat (Triticum aestivum L.; AABBDD) and Aegilops squarrosa L. (DD) or T. urartu Thum. (AA), this bottleneck may be overcome simply by pollinating a sufficient number of F₁ spikes. However, hybrids between hexaploid wheat cultivars (T. aestivum) and T. monococcum L. (AA) generally are highly female-sterile, often having no pistils. One T. monococcum accession, PI 355520, when crossed with T. aestivum, produced hybrids with female fertility in the same range as that of T. aestivum/A. squarrosa or T. aestivum/T. urartu hybrids, ca. 0.5 to 1.0 backcross seed per spike. We found that female fertility was controlled by two duplicate genes in PI 355520, and that this accession can be used as a bridging parent to introgress genes from other T. monococcum accessions into hexaploid wheat. Pairing of homologous chromosomes was less frequent and weaker in such crosses than in T. aestivum/A. squarrosa crosses, but homoeologous bivalents occurred at a rate of almost 0.5 II per cell. Restitution division was detected in crosses involving all three diploid species and was confirmed cytologically in crosses with PI 355520. Chromosome numbers of BC₁F₁ plants ranged from 35 to 67; plants with 49 or more chromosomes occurred at frequencies of 0.09 to 0.21 among progeny of A. squarrosa and T. urartu and 0.29 in progeny of T. aestivum/T. monococcum crosses involving PI 355520. These results are consistent with those of previous studies, demonstrating the potential of direct Hexaploid/diploid crosses for rapidly introgressing useful genes into Hexaploid wheat with minimum disturbance of the background genotype.     

Breeding behavior for tuber protein in Solanum tuberosum and Tuberosum-phureja hybrids

Summary True protein content among tubers within a plant of Solanum tuberosum cv. Oneida was found to be negatively correlated with tuber size. A similar study of S. phureja genotype 148-17 revealed no correlation. Tuber protein was determined for 250 genotypes in each of four hybrid potato populations in a factorial mating design with four potato cultivars as stylar parents mated to four groups of pollen parents (4x cultivars, 4x high protein selections, 2x S. phureja, 2x high protein S. phureja). The hybrid population derived from the 4x high protein selections was significantly higher in protein content than the other populations. High estimates of general combining ability for tuber protein content were found for both stylar and pollen parents. Poor photoperiodic adaptation to growing conditions in northern latitudes was a possible explanation for the variable protein phenotypes typical of S. phureja and the lack of transmission of the high protein character in phureja to 4x-2x hybrids. Although protein content was negatively correlated with total yield, high protein segregates with good yield potential were identified in all four populations.Scientific Journal Series Article 11, 616 of the Minnesota Agricultural Experiment Station.     

中国普通小麦生态区划及生态分类Ⅰ中国普通小麦生态区划

提出了由小麦气候生态区、地理生态区、栽培生态区及品种区域四级所构建的小麦生态区划体系。将中国普通小麦划分为５大气候生态区和２０个地理生态区，即：Ⅰ北方温带春麦气候生态区，包括东北、华北和西北３个春麦地理生态区；Ⅱ黄淮海暖温带冬麦气候生态区，包括海河流域、黄土高原、胶东沿海、华北平原、黄河中游丘陵川地和淮北平原６个冬麦地理生态区；Ⅲ南方亚热带（热带）秋（冬）播春麦气候生态区，包括长江中下游平原、秦巴山地、南方山地、四川盆地、云南高原、川贵高原６个秋播春麦地理生态区和华南冬播春麦地理生态区；Ⅳ新疆内陆冬春麦气候生态区，包括北疆春冬麦和南疆冬春麦两个地理生态区；Ⅴ青藏高原春冬麦气候生态区，包括青海春麦和西藏冬春麦两个地理生态区。     

地理信息系统在县级农业气候区划中的应用

利用City Star(3.0)地理信息系统软件和广西1：25万基础地理数据，开展了容县沙田柚种植的农业气候区划，取得了较好的效果。提出了应用GIS技术开展县级农业气候区划的新思路、新方法和具体步骤。     

中国西北部生态脆弱区典型区域土地综合整治分区——以甘肃省兰州市为例

[目的]探讨土地整治规划的重要内容——土地整治分区,为确定土地整治重点区域和土地整治规划提供参考依据。[方法]以西北部生态脆弱区典型区域甘肃省兰州市为研究区域,基于生态保护视角,在充分考虑区域自然地理特征、土地利用特点及区域主要生态问题的基础上,从地形地貌、降雨、土壤类型、植被类型、水土流失、土地利用等5方面选取指标因子初步构建分区指标体系,并从水资源供给、水系分布及行政区划等方面进一步完善。同时运用ArcGIS空间叠加分析功能,使用综合分析法进行土地综合整治分区。[结果]形成了"方位+地貌+土地利用类型"命名的土地整治两级分区体系,二级分区较为详细。兰州市划分为7个一级区和15个二级区。[结论]中国西北部生态脆弱区域在土地综合整理过程中应该通过选择合理的指标体系分区进行整治。各分区应该针对其土地资源、水资源、生态环境状况等具体特征,基于生态保护视角采取有效措施进行土地综合整治。     

五常市北部地区农村居民点用地适宜性评价与整治分区规划

农村居民点用地适宜性评价是实现农村土地合理布局、挖掘土地整理潜力的重要依据。以五常市北部地区为例,从自然、经济社会和生态三方面构建典型粮食产区农村居民点用地适宜性评价指标体系,结合地区内部特点量化指标因子;依据适宜性评价分级和区域模式差异,综合考虑人口城镇化影响实现居民点的整治分区规划。结果表明:北部12个乡镇中农村居民点用地适宜性区间为0~3.996 8,不存在最高适宜的居民点用地,域内以高适宜和中适宜用地为主,低适宜和不适宜的居民点用地相对比例之和5.02%,地区仍有部分居民点需重点整治;兴隆乡、营城子乡、民乐乡等部分地区应引导村内人口外迁,八家子乡、常堡乡、牛家镇等应积极迁村并点,兴隆乡部分地区、八家子乡和卫国乡实施内部改造,保留发展型均有分布。研究结果可为该地区居民点整理规划提供科学依据。     

食用菌仿野生栽培在北京生态涵养发展区的作用与展望

食用菌仿野生栽培能够有效利用人工林和天然林的大环境,产品天然优质。介绍了北京生态涵养发展区野生菌原始的生长状态、食用菌仿野生栽培的环境优势、仿野生栽培的作用、仿野生食用茵产品的优缺点、仿野生栽培适宜的种类、技术和发展前景等．