Production of hybrids betweenCajanus platycarpus andCajanus cajan

Summary Cajanus platycarpus a wild species of pigeonpea incompatible with the cultigen has many desirable characters important for the improvement of cultivated pigeonpea. In the present study, barriers to hybridization were studied and were identified as post-zygotic. Efficient embryo rescue techniques were developed. As a result, hybrids were produced. Morphological and cytological studies as well as esterase isozyme band pattern confirmed the hybrid nature of the plants. The F₁ hybrids were completely pollen sterile. Meiotic studies were carried out to check for the cause of pollen sterility.Abbreviations MS Murashige & Skoog's (1962) medium - BAP 6-Benzylaminopurine - IBA Indole-3-butyric Acid - NAA Naphthaleneacetic Acid     

甘蓝型油菜生态雄性不育系533S花药发育的细胞学研究

对甘蓝型油菜生态雄性不育系533S的花药发育过程进行了石蜡切片和显微观察,结果表明:533S的花药发育受阻于孢原细胞时期,其主要特点是不能形成正常的孢原细胞进而分化出花粉囊,属无花粉囊型不育系。在败育过程中,花药的大小一直在增加,但始终无花粉囊的分化。随着生态条件的变化,533S的部分花药可分化出1～2个花粉囊,产生少量可育花粉粒。最终,随着生态条件的进一步变化,花药的发育完全正常,产生大量正常可育花粉粒。     

小麦白粉菌早期发育与初侵染行为的细胞学研究

病菌孢子的萌发不受寄主专化抗性的影响，在叶面及玻片上均可正常进行；但有些抗源材料可抑制附着胞分瓣的正常分化；附着胞分瓣的形成需要一定的接触刺激，否则将导致产生纤细附着胞；病菌附着胞的多分瓣现象是其多次入侵的表现；高抗性小麦品种多在病菌吸器产生前发生过敏性坏死反应；乳突频率的高低与乳突抗性强弱无必然联系。     

芍药的研究进展

对芍药的起源、分类,栽培技术、繁殖技术、引种培育状况、病虫害防治,鲜切花贮藏保鲜,细胞学及分子生物学研究,化学成分检测提取技术等方面的研究进展状况进行了论述,总结了目前芍药研究尚无定论的问题及有待深入的领域.     

不同类型雄性不育苎麻细胞学机理研究

为探明苎麻雄性不育材料和可育材料在花芽分化和花药发育上的差异，选取不可育材料雄花萎缩型园青5号、花被不开裂型弯子苎麻、花粉不育型黄小叶和可育材料中苎1号(对照)，采用常规石蜡切片技术对其进行雄花花芽发育形态比较。结果表明：3种雄性不育苎麻花芽发育较可育苎麻中苎1号快，在花芽发育到0.3~0.5 cm时，已完成了雌、雄蕊的分化。参照可育苎麻材料的花芽分化和花药发育，推测3种雄性不育材料不育的原因分别为园青5号花芽在分化出花药和花丝后，花药不会继续分化形成花粉囊，而是变形萎缩甚至消失形成空腔；弯子苎麻在花粉分化阶段花被木质化严重；黄小叶花粉囊不破裂，花粉粒颜色逐渐变深，花丝发育异常。     

Pot-bellied Vietnamese pig with visceral mast cell tumors and mastocytemia

An approximately 12-year-old female Vietnamese Pot-Bellied Pig was presented to the Mississippi State College of Veterinary Medicine Food Animal Service for anorexia of 2 days duration. On physical examination, the patient appeared depressed and lethargic with significantly pale mucus membranes, open mouth breathing, and nostril flaring. On abdominal palpation, the abdomen was tense and uncomfortable. A complete blood count (CBC) and chemistry profile were performed. The CBC revealed significant anemia and mild leukocytosis characterized by mild neutrophilia with a left shift. Mast cells were rarely observed. Hematocrit = 8.1% (RI 22-50), RBC = 1.25 × 10⁶/μL (RI 3.6-7.8), WBC = 19.85 × 10³/μL (RI 5.2-17.9), Neutrophils = 15.08 × 10³/μL (RI 0-11.4), and Bands = 0.993 × 10³/μL (RI 0-0.019). The chemistry profile was unremarkable with a mildly elevated BUN and slightly decreased total protein and albumin (BUN = 39 mg/dL [RI 4.2-15.1], total protein = 6.2 g/dL [RI 6.6-8.9], and albumin = 2.5 g/dL [RI 3.6-5.0]). An abdominal ultrasound revealed numerous hypoechoic nodules diffusely scattered throughout the hepatic parenchyma. An FNA of one of the hepatic nodules was performed. A mild suppurative component and numerous variably granulated mast cells were observed. A presumptive cytologic diagnosis of mast cell tumor was made. Histopathology was performed, confirming the cytologic interpretation.