刺参生殖腺发育的生物学零度和有效积温研究

[目的]为刺参的繁殖以及工厂化人工育苗提供理论基础。[方法]以渤海湾地区刺参为试验材料,研究渤海湾地区刺参生殖腺发育生物学零度及其性成熟的有效积温。[结果]渤海湾地区刺参生殖腺发育的生物学零度为6.14℃,而有效积温为800.19℃·d。[结论]该研究在渤海湾地区刺参育苗生产中具有重要的参考价值。     

基于机载LiDAR数据的玉米叶面积指数反演

以机载LiDAR离散点云数据为数据源,基于植被冠层孔隙率与叶面积指数的关系,提出一种反演大田玉米叶面积指数的方法。对反演LAI和实测LAI进行对比分析,结果表明:基于Axelsson改进的不规则三角格网加密方法可以将地面点和非地面点分开,结合高分辨率影像能够提取出玉米冠层点云;基于孔隙率反演LAI,尼尔逊参数的选择对结果影响很大,利用扫描天顶角模拟尼尔逊参数,LAI反演结果接近于真实情况。利用机载LiDAR点云数据能精确地反演大田玉米LAI,该研究方法适用于中等高度的农作物,可以扩展到甜菜、甘蔗等其他中等高度农作物。     

云南佤族文化旅游资源开发

分析了佤族的司岗里文化、猎头风俗以及其他独特文化的旅游资源价值,认为长期以来佤族旅游由于交通不便、开发意识不足、宣传力度不够等原因使这些资源处于沉睡状态。因此,在旅游需求、通达条件、国家西部大开发深度都已发生深刻变化的今天,主张抓好相关设施建设,做好宣传工作,不仅将大力促进这些地方旅游业发展,还将为广大旅游消费者提供高品质的旅游商品。     

景泰绿洲3种小谷物的产草量与牧草营养成分预测

为了预测景泰绿洲春小麦(Triticum aestivum)、燕麦(Avena sativa)、黑麦(Secale cereale)的产草量和营养品质,通过田间试验,测得牧产草量、株高和分蘖,室内测定粗蛋白、粗脂肪、中性洗涤纤维、酸性洗涤纤维、粗灰分和可溶性碳水化合物,分析各指标之间的相关性。结果表明,收获干草的3种小谷物分蘖和株高均与可溶性碳水化合物之外其它5个营养指标存在显著相关性(P0.05),模拟轮牧的春小麦和黑麦株高与营养指标均无相关性(P0.05),燕麦和黑麦的分蘖与6个营养指标均呈显著相关(P0.05)。建立了根据株高预测产草量和营养品质的模型、用分蘖预测牧草营养品质的模型、用产草量预测牧草营养品质的模型、用分蘖和产草量两个因子综合预测营养品质的模型。经过与实测值对比,各预测模型的准确性均较高。     

经络穴位给药系统在兽医临床上的应用

经络穴位给药系统以中医经络理论为基础,具有经穴效应和药物效应的双重治疗特性,已广泛应用于兽医临床.目前,兽医临床上常用穴位给药方式是穴位埋植和穴位注射,而常用穴位主要是后海穴、百会穴、肺龠穴.随着经络穴位给药系统的广泛应用,新型经络穴位给药制剂和经络穴位给药作用机理已成为经络穴位给药系统研究的重点.     

论影响麻花钻切削因素之间的关系

学生搞不清楚麻花钻刃磨时几何角度之间的关系,导致麻花钻头的刃磨失败,最终使学生形成了麻花钻内容太难,麻花钻刃磨起来太麻烦的结论。用图示、口诀配合文字讲解的方式讲解标准麻花钻切削部分之间的关系,学生易于接受。     

家庭猪场防控非洲猪瘟的关键点分析

非洲猪瘟对家庭猪场的生产稳定性带来极大的挑战。文章分析了家庭猪场防控非洲猪瘟存在的问题及对关键点针对性的防控措施。     

Construction and Identification of Yeast Surface Display Libraries of Ovis aries DRA Gene Exon 2

The specific primers were designed according to Ovis aries DRA gene sequence deposited in GenBank and the multiple cloning site of the plasmid pYD1,which was a vector used for protein surface display on Saccharomyces cerevisiae.The gene encoding DRA was amplified by PCR using the genomic RNA of Ovis aries.The 762 bp fragment was cloned and released in GenBank and registration number was KR422362.The PCR product was inserted into the yeast surface display plasmid vector pYD1 by double enzyme digestion.It was indicated that DRA gene was successfully integrated into the genome.Dot mutation was made at both ends of exon 2 in DRA gene for making restriction enzyme cutting site and design the exon 2 specific primers according to mutated Ovis aries DRA gene sequence.Sequenced exon 2 amplification products based on DNA pooling of sheep large sample template was analyzed the polymorphic loci.The polymorphic exon 2 246 bp fragment was obtained by double enzyme digestion and connected to surface display restructuring mutation carriers pYD1-DRA by the same double enzyme digestion,and then we successfully constructed yeast surface display libraries.We transformed it into Saccharomyces cerevisiae EBY100 cell.Yeast monoclone was identified by PCR amplification and sequencing,and we confirmed that DRA gene had been integrated into Saccharomyces cerevisiae genome.After galactose induced,it was detected that DRA gene library had been successfully demonstrated on the yeast cell surface under the fluorescence microscope by immunofluorescence method.     

ASVCP guidelines: quality assurance for portable blood glucose meter (glucometer) use in veterinary medicine

Portable blood glucose meters (PBGM, glucometers) are a convenient, cost effective, and quick means to assess patient blood glucose concentration. The number of commercially available PBGM is constantly increasing, making it challenging to determine whether certain glucometers may have benefits over others for veterinary testing. The challenge in selection of an appropriate glucometer from a quality perspective is compounded by the variety of analytic methods used to quantify glucose concentrations and disparate statistical analysis in many published studies. These guidelines were developed as part of the ASVCP QALS committee response to establish recommendations to improve the quality of testing using point‐of‐care testing (POCT) handheld and benchtop devices in veterinary medicine. They are intended for clinical pathologists and laboratory professionals to provide them with background knowledge and specific recommendations for quality assurance (QA) and quality control (QC), and to serve as a resource to assist the provision of advice to veterinarians and technicians to improve the quality of results obtained when using PBGM. These guidelines are not intended to be all‐inclusive; rather they provide a minimum standard for management of PBGM in the veterinary setting.     

Optimising cutting method and timing for the control of Abutilon theophrasti seed production

Abutilon theophrasti (velvetleaf) is a widespread and problematic annual weed. Greenhouse and field experiments were conducted to investigate the effects of different cutting methods on the viability of A. theophrasti seeds. Three cutting methods were assessed: (1) Entire plant cut and dried (EPD)—plants were cut at soil level and dried with capsules attached on the greenhouse bench or soil surface for 4 weeks; (2) capsules detached and dried (CD)—capsules were removed from plants and dried for 4 weeks; and (3) capsules detached and tested while fresh (CF)—a control treatment. Before drying, the developmental stage (stage one, dark green; stage two, light green; stage three, yellowish-green; or stage four, black with the slightly open capsule) and age (days after flowering, DAF) of each capsule was recorded. Seed viability was measured immediately in the CF treatment and after the 4-week drying period in the EPD and CD treatments. No seeds in the EPD and CD treatments were viable when harvested at the first developmental stage (1–8 DAF) in either experiment, but 100% of seeds in the CF treatment in the field were viable when harvested at 8 DAF. In both greenhouse and field experiments, seeds attained full viability at earlier harvest ages in CF than in EPD or CD treatments, suggesting that seeds might become viable relatively early in development but lose viability if allowed to dry. These findings could be applied to optimise late-season mechanical control of A. theophrasti.