Behavior of phenolic substances in the decaying process of plants

The behavior of phenolic substances in the decaying process of rice straw, ladino clover, and fanen leaves of red oak under moist conditions, and also of rice straw under various conditions were compared in the laboratory. The amounts of phenolic substances, divided into either humic acid and fulvic acid fractions, or ether-extractable, butanol-extractable and organic solvent-unextractable fractions, and the amounts of individual phenolic acids were periodically determined during incubation for 150 days. The following results were obtained.

1) The amounts and behavior of phenolic substances in various fractions differed considerably among the plant materials. The total amount of phenolic substances was remarkably larger in red oak leaves than in the others during the whole period of incubation. The amounts of phenolics in the fulvic acid fraction changed to a larger extent than those in the humic acid fraction during the decaying process of plant materials. The changes in total amount of phenolics in decaying red oak leaves and ladino clover were mainly due to changes in the level of relatively hydrophilic phenolics in the fulvic acid fraction, but the changes in decaying rice straw were mainly due to changes in the level of relatively lipophilic phenolics.

2) Rice straw and ladino clover, especially the former, contained large amounts of p-coumaric and ferulic acids, but these decreased rapidly in the early stage of the decaying process. The amounts in red oak leaves were small, but did not decrease markedly during incubation.

3) The changes in amounts of phenolics in both humic acid and fulvic acid fractions in the decaying process of rice straw were largely influenced by temperature, moisture, and pH, but not to a large extent by C/N ratio and the presence of soil. At higher temperatures under moist conditions, phenolic substances disappeared rapidly. Also, acidification of the system inhibited the degradation process.     

Effect of rice variety on methane emission from an Indonesian paddy field

Variations in CH₄ emission from a Sumatra paddy field in which 8 popular modern varieties in Indonesia were grown were compared in the 1994/1995 rainy season. Total amounts of CH₄ emitted during the period of rice growth were in the ranges of 32.6-41.7 and 51.3–64.6 g CH₄ m^-2 for the plots amended with chemical fertilizer only and those amended with both rice straw and chemical fertilizer, respectively. The mean CH₄ emission rate was highest in the plot with the variety Bengawan solo and lowest in the plots with the varieties Atomita-4 and Way seputih among the plots which received chemical fertilizer, while highest in the plot with Way seputih and lowest in the plot with Bengawan solo among the plots amended with both rice straw and chemical fertilizer. The increase in the mean CH₄ emission rates by rice straw application was higher for the plots planted with Way seputih (1.98 times) and Atomita-4 (1.77 times) than for the plots with Bengawan solo (1.23 times) and IR-64 (1.35 times). The plots with Walanai and Cisanggarung recorded intermediate mean emission rates and the increase in CH₄ emission by rice straw application was also intermediate (1.57–1.64 times). It was noteworthy that Way seputih and Atomita-4 were derived from the variety Cisadane, Bengawan solo and IR-64 from the variety IR-54, and Walanai and Cisanggarung from the varieties IR-36 and Pelita 1-1, respectively.

The amounts of CH. emitted for 1 kg grain production ranged from 53 (Atomita-4) to 74 (Kapuas and Walanai) and from 89-93 (IR-64, Bengawan solo, and Atomita-4) to 121 (Kapuas) g CH₄ kg^-1 of grain for the plots amended with chemical fertilizer and those amended with rice straw and chemical fertilizer, respectively.     

温度对麦秸混合物料厌氧干发酵中糖类水解酶活性的影响

水解酶活性直接影响到秸秆厌氧干发酵的沼气产量。本文以麦秸为主要原料, 配制干物质含量(TS)35%的混合发酵物料, 设置20 ℃、25 ℃、30 ℃、35 ℃、40 ℃ 5种不同温度条件, 发酵周期为35 d, 测定发酵过程中料液的纤维素酶、木聚糖酶、淀粉酶和蔗糖酶的活性变化。结果表明, 沼气产气高峰在35 ℃左右, 20~40 ℃时, 随着温度的升高, 发酵后料液的料液干物质含量(TS)、可挥发固体含量(VS)、C/N均明显下降, 发酵产沼气后, 各处理发酵液 pH都降到7.0以下, 其中40 ℃的pH降低幅度最大。纤维素酶活性在发酵初期较低, 峰值出现也较晚, 温度升高明显有利于提高纤维素酶活性。木聚糖酶的起始活性较高, 整个发酵过程中呈先升高后降低并渐趋于平缓的变化趋势, 20~25 ℃处理的木聚糖酶活性在整个发酵过程中没有明显峰值。淀粉酶的起始活性较低, 但随后迅速升高, 30~40 ℃处理在10 d左右达到峰值后开始下降, 此后酶活力的变化幅度较前期平稳。蔗糖酶的起始酶活力较低, 随后升高迅速, 温度在30 ℃以上时酶活性增加更快, 35 ℃时15 d达到峰值后迅速下降, 下降速度明显大于30 ℃和40 ℃处理。由此可见, 温度对秸秆厌氧干发酵过程中不同水解酶活性的影响还是有所差异的。     

冻融期秸秆覆盖量对土壤剖面水热时空变化的影响

为了揭示季节性冻融期秸秆覆盖量对土壤剖面水热时空变化的影响,分析对比了裸地和5种不同玉米秸秆覆盖厚度(5、10、15、20和30 cm)地块的土壤剖面含水率和土壤温度等值线变化特征,采用数理统计分析方法对土壤剖面水热变化进行了统计学分析。结果表明:在季节性冻融期,裸地最大冻结深度为52 cm,土壤剖面水热变化较为剧烈,0~40 cm属于水热变化活跃层,覆盖厚度为5和10 cm时的土壤剖面水热变化活跃层分别为0~20和0~10 cm。秸秆覆盖厚度为15 cm时可平抑土壤剖面水热的变化,并能达到良好的保温效果。秸秆覆盖厚度为5 cm时,在土壤冻融作用和秸秆覆盖的双重效应下,耕作层土壤水分较其他地块高,储水保墒效果显著。当秸秆覆盖厚度大于15 cm时,土壤保墒保温效果不随秸秆覆盖量的增加而增强。从预防冻害和蓄水保墒角度出发,最佳秸秆覆盖厚度为10~15 cm。研究成果对于季节性冻土地区冬春季节农田秸秆覆盖的科学实施具有重要的指导意义。     

秸秆还田下氮肥运筹对白土田水稻产量和氮吸收利用的影响

【目的】研究小麦秸秆直接还田条件下不同氮肥基追比例运筹方式对白土稻田水稻产量和氮素吸收利用的影响, 为华中低产白土稻田水稻合理施肥提供科学依据。【方法】设置2种小麦秸秆还田量(0和3000 kg/hm2)及3种氮肥基肥-分蘖肥-穗肥施用比例(80-0-20、 60-20-20 和40-30-30)和不施氮的对照, 共7个处理, 分别为N80-0-20、 N60-20-20、 N40-30-30、 N80-0-20+S、 N60-20-20+S、 N40-30-30+S和CK。水稻收获期采集代表性样品考察水稻产量结构性状, 同时测定水稻籽粒和秸秆产量, 分析籽粒和秸秆氮素含量, 计算水稻氮素吸收量和氮肥利用效率。【结果】基肥-分蘖肥-穗肥施用比例60-20-20的处理水稻籽粒产量最高, 两年试验较不施分蘖肥的对照分别增产9.4%~12.9%和7.4%~8.9%。实施小麦秸秆直接还田后, 水稻籽粒产量较不施秸秆的对照分别提高10.2%~23.4%和0.8%~5.5%。不施秸秆条件下, 基-蘖-穗肥施用比例60-20-20的处理水稻籽粒含氮量最高, 较不施氮的对照提高11.3%, 而秸秆含N量随中后期追肥比例的加大而提高。秸秆还田条件下, 氮肥后移能明显提高水稻籽粒和秸秆含氮量。水稻籽粒氮素吸收量, 基-蘖-穗肥比例60-20-20处理最多, 2011年较对照N80-0-20分别增加13.7%和24.8%, 2012年提高14.5%和9.2%; 秸秆氮素积累量则随中后期追肥用量的增加而增多, 基-蘖-穗肥比例40-30-30处理最多。不施秸秆条件下, 基-蘖-穗肥比例60-20-20的处理氮素干物质生产效率、 氮素稻谷生产效率、 氮收获指数均最高, 百公斤籽粒吸氮量最低。秸秆还田条件下, 氮素干物质生产效率和氮素稻谷生产效率均随中后期追肥量的增加而下降, 而百公斤籽粒吸氮量则最高。氮素农学效率、 氮肥回收利用率和偏生产力也是60-20-20比例的处理最高, 较对照N80-0-20农学效率分别提高4.90和2.44 kg籽粒/kg N, 氮肥利用率提高7.82和21.29个百分点, 偏生产力提高4.90和2.44个百分点。【结论】综合水稻产量、 氮素吸收量以及氮肥利用效率, 安徽省江淮丘陵低产白土地区, 小麦秸秆直接还田条件下, 单季中稻氮肥的基肥-分蘖肥-穗肥施用比例, 以60-20-20运筹方式较为适宜。     

适量有机肥与氮肥配施方可提高河西绿洲土壤肥力及作物生产效益

【目的】 河西绿洲灌溉农业区是我国主要的商品粮生产及玉米制种基地,结合当地耕作制度进行土壤有效培肥是提高农业生产效益和可持续发展的基础。本研究以河西灌漠土长期定位试验为依托,探讨了长期施用一种或者多种有机肥以及有机无机肥配合对土壤养分、土壤酶活性及产量的影响,为筛选出适于当地农业可持续发展的施肥模式提供理论依据。 【方法】 试验采用随机区组排列,除对照外,在施磷肥 (P₂O₅) 150 kg/hm2 的基础上,再设 12 个处理:单施用农家肥 120 t/hm2 (M);单施绿肥 45 t/hm2 (G);单施秸秆 10.5 t/hm2 (S);单施氮肥 375 kg/hm2 (N);农家肥 60 t/hm2 + 绿肥 22.5 t/hm2 (1/2MG);农家肥 60 t/hm2 + 秸秆 5.25 t/hm2 (1/2MS);农家肥 60 t/hm2 + 氮肥 187.5 kg/hm2 (1/2MN);绿肥 22.5 t/hm2 + 氮肥 187.5 kg/hm2 (1/2GN);秸秆 60 t/hm2 + 氮肥 187.5 kg/hm2 (1/2SN);农家肥 40 t/hm2 + 绿肥 15 t/hm2 + 氮肥 124.5 kg/hm2 (1/3MGN);农家肥 40 t/hm2 + 秸秆 3495 kg/hm2 + 氮肥 124.5 kg/hm2 (1/3MSN);农家肥 30 t/hm2 + 秸秆 2625 kg/hm2 + 绿肥 11.25 t/hm2 + 氮肥 94.5 kg/hm2 (1/4MGSN)。调查了耕层土壤有机质和速效养分含量变化以及几种主要土壤酶活力,采用主成分分析、聚类分析、经济效益分析综合比较了不同施肥方式对土壤质量与经济收益的影响。 【结果】 长期不施肥、单施氮肥(N)、氮肥配施绿肥或者秸秆均会造成土壤钾素匮缺,较 1988 年初测土土壤速效钾含量分别下降了 16.59%、39.37%、25.04%、23.31%;M、G、S 三种有机肥单施或与氮配施均能提高土壤碱解氮、土壤有效磷、土壤有效钾、土壤有机质(SOM)含量,不同施肥方式下,其含量大小总体表现为高量有机肥 > 减量有机肥 + 减量 N > N > CK;M、G、S、N 单施或 1/2MN、1/2GN、1/2SN 处理均能提高蔗糖酶、磷酸酶、脲酶、过氧化氢酶活性,以 G、1/2GN 处理对提高土壤蔗糖酶、脲酶的活性的效果最显著,M、G、MN、GN 处理对提高碱性磷酸酶活性的效果最显著;主成分得分进行聚类分析,不同处理培肥土壤质量效果由高到低分成四类,分别为一类高量有机肥 M、G、1/2MG > 二种有机肥与氮肥减量配施 1/2MN、1/3MSN、1/3MGN、1/2GN、1/2MS、1/4MSGN > 三类氮肥、秸秆、秸秆与氮肥 N、1/2SN、S > 四类不施肥(CK);各施肥方式均能提高作物产量,与不施肥(CK)相比,增产幅度为 12.21%～235.4%,肥料贡献率在 10.89%～70.18% 之间,单施肥增产总趋势为 N > G > M > S,减量配施肥增产总趋势为 1/2GN > 1/2MN>1/2MS,施肥方式以有机肥配施 N 对产量贡献最大;经济效益分析表明施 N 或有机肥与 N 配施对于提高净收益作用较大。 【结论】 通过主成分分析与聚类分析、经济效益与产量综合比较,农家肥、绿肥和秸秆长期单施成本高,产量和经济效益低,维持土壤养分和产量需要的用量大。因此,提倡适量有机肥与氮肥配施,达到提高作物产量,增加经济效益,保证土壤肥力可持续发展。      

Diversity and abundance of springtails (Hexapoda: Collembola) in soil under 90-year potato monoculture in relation to crop rotation

The monoculture cropping system causes significant changes within the soil ecosystem, which constitutes a habitat for soil-dwelling springtails. Focusing on the response of soil fauna to 90 years of potato cultivation in monoculture the study investigates the abundance and diversity of soil-dwelling springtails, considering changes in the soil environment in relation to five-crop rotation. Another point was the soil quality evaluation using Collembola as bioindicators (QBS-c index). A long-term monoculture experiment was established in Poland in 1923 and has continued uninterruptedly to the present time. Soil samples were taken over a period of three years (2011–2013) to determine collembolan abundance and composition, as well as physical and chemical soil properties.

The study demonstrated that there were greater numbers of Collembola in the long-term monoculture of potatoes, especially before planting time, compared to numbers in a five-field crop rotation. At the same time apparently greater species diversity was found in potato culture within crop rotation. The biological indicator of soil quality based on the occurrence of springtails (QBS-c) has proved useful in assessing changes in soil caused by agrotechnical activities. This index indicated better biological soil quality in the five-field rotation system compared to monoculture.     

绿洲灌区小麦免耕秸秆还田对后作玉米产量性能指标的影响

产量性能是决定作物生长发育和产量形成的关键因素,研究前茬秸秆处理方式对后茬作物产量性能指标的影响,对于建立高效种植制度、优化栽培措施具有重要指导意义。2009—2012年,在甘肃河西绿洲灌区,通过田间定位试验,研究了前茬小麦不同秸秆还田和耕作措施(NTSS:25 cm高茬收割立茬免耕;NTS:25 cm高茬等量秸秆覆盖免耕;TIS:25 cm高茬等量秸秆翻耕;CT:不留茬翻耕对照)对后作玉米产量性能指标的影响,以期为优化试区玉米种植模式提供依据。结果表明,与CT相比,前茬小麦秸秆还田降低了后作玉米大喇叭口期之前的叶面积指数(LAI)与光合势(LAD),但增大了吐丝期之后的LAI与LAD,延缓了衰老,以NTSS、NTS延缓衰老作用突出;NTSS、NTS和TIS处理玉米全生育期的平均叶面积指数(MLAI)比CT分别提高12.8%、19.1%和7.0%,总光合势分别提高12.9%、18.6%和6.8%,免耕秸秆还田(NTSS和NTS)提高MLAI和LAD的效果最好。免耕秸秆还田提高了玉米全生育期的平均净同化率(MNAR),以NTSS提高作用明显,较CT高10.7%;但净同化率(NAR)表现为吐丝期之前增大,吐丝期之后降低。NTSS、NTS提高了后作玉米的籽粒产量,比CT分别高13.0%、15.6%,TIS比CT提高7.9%,NTS增产效应最大。不同秸秆还田及耕作方式下,玉米籽粒产量与MLAI、穗数(EN)、穗粒数(KNE)呈极显著正相关性,与收获指数(HI)呈显著正相关性,但与MNAR无显著相关性。MLAI、EN、KNE增加、HI提高是前茬小麦免耕秸秆还田提高后作玉米产量的主要原因。前茬秸秆免耕还田优化后茬玉米主要产量性能指标的效果最好。因此,前茬小麦25 cm秸秆覆盖免耕还田是绿洲灌区优化后作玉米产量性能指标及获得高产的可行栽培措施。     

热解油水相酸洗-烘焙二级预处理改善玉米秸秆热解特性

烘焙和酸洗都是可提升生物质品质的预处理方法。烘焙可以脱除生物质中的氧,酸洗则可有效脱除碱金属及碱土金属,而氧和AAEMs对热解油的品质和产率均具有影响。该文研究了酸洗-烘焙两级耦合预处理对玉米秸秆热解特性的影响。试验用酸液取自热解联产联供示范项目热解油的水相部分,烘焙温度选取230、260、290℃。研究发现,酸洗预处理能够有效脱除AAEMs,对K、Na、Mg脱除率分别达到97.53%、81.38%、84.86%。两级预处理能明显降低O/C;酸洗-290℃烘焙半焦相比玉米秸秆原样,O/C降低了25.32%。两级预处理能明显削弱烘焙对热解油产率的不利影响,酸洗-290℃烘焙半焦相比290℃烘焙半焦,其热解油产率提高127.66%;两级预处理显著提高了热解油中糖类的含量,同时降低了酚类和酸类的含量;对酸洗-290℃烘焙半焦,其热解油中糖类含量高达45.89%,酚类和酸类则低至9.76%和6.31%。其他化学组成如酮类和呋喃类的含量存在一定程度的下降,醛类含量则有小幅度的提升。该文提出的利用热解联产联供示范项目热解油的水相部分对秸秆进行酸洗,并结合烘焙的两级预处理方法可为对生物质热解提供参考。     

大型秸秆沼气集中供气工程温室气体减排估算

发展秸秆沼气工程可有效地减少农业温室气体排放,科学核算温室气体减排量为管理和监督温室气体排放状况提供数据支撑。该文以河北省沧州市耿官屯大型秸秆沼气集中供气工程为研究对象,参考和借鉴了自愿减排项目方法学、CDM方法学,构建了大型秸秆沼气集中供气工程温室气体减排计量方法,包括项目边界、基准线排放量、项目排放量、泄漏量、减排量5个方面,计算了2014年耿官屯大型秸秆沼气集中供气工程温室气体减排量。研究结果表明:项目基准排放量包括秸秆处理产生的温室气体排放、未建秸秆沼气工程情况下农村居民生活用能及农田施用化肥生产耗能产生的温室气体排放。项目排放量包括秸秆与沼肥运输过程耗能排放、工程运行过程耗能排放及沼气处理温室气体排放,项目泄漏量即沼气生产、储存、管网供气和利用过程中产生的因物理泄漏所造成的排放。2014年耿官屯大型秸秆沼气集中供气工程基准线CO2排放量为5 776.15 t,项目排放量为57.53 t,泄漏量为136.59 t,减排量为5 582.03 t,约相当于2 100 t标准煤CO2排放量,每消耗1 t(干质量)秸秆可净减排3.56 t,每利用1 m3沼气可净减排11.50 kg。同时,在工程设计、管道设计、工程管理、工艺技术改良升级等方面提出了提升大型秸秆沼气工程温室气体减排能力的策略。