不同比例缓释肥、烟草专用复合肥配施对植烟土壤肥力及烤烟产质量的影响

为探究不同比例缓释肥、烟草专用复合肥配施对植烟土壤肥力及烤烟产质量的影响,在宣威市热水镇植烟区设置了5种不同肥料配比组合和对照,对植烟土壤及烤后烟叶进行取样分析。结果表明：不同比例缓释肥配施烟草专用复合肥使土壤综合肥力指数(IFI)相较于CK（单施烟草专用复合肥）提高了12.50%~23.21%,以60%~100%缓释肥等比替代复合肥(T3、T4、T5)表现最优。从烤烟综合品质来看,不同比例缓释肥替代烟草专用复合肥使烤烟综合品质平均得分相较于CK提高了0.19%~6.64%,其中以80%缓释肥替代复合肥处理(T4)表现最优。烤后烟叶经济效益也以T4最优,公顷产值达到59995.80元,高于对照和其他处理。综上所述,缓释肥和复合肥比例为80%:20%,能较好的提高烟叶综合品质,烤后烟叶能获得较好的经济效益,并在一定程度上改善土壤养分结构。     

浅析等离子体催化联合脱硝机理研究

主要描述了现有等离子体催化脱硝的机理和现有催化剂的种类，通过对脱硝机理的研究和催化剂的选择，发现了其中的一些具有较好的脱硝效果的催化剂如Al2 O3负载型催化剂、分子筛催化剂（ZSM －5）等，有的甚至能达到90％以上，针对研究中发现有些方面还是空白，提出了未来等离子体催化联合脱硝的研究方向。     

基于联合仿真的林业采育作业模拟系统研究

为解决大型林业装备操作训练复杂的问题,优化驾驶与作业,提高森林资源利用率,本文提出基于分布式系统,对采育作业环境进行视景模拟的虚拟现实作业操作模拟器。系统利用虚拟现实软件Unity3D系统,能够模拟车辆行驶与工作的动力学模型,实时运动仿真包括底盘系统与地面的交互作用,机械臂作业运动以及伐木头作业操作运动,使操作人员能够更快地适应熟悉驾驶和采育作业,减少安全隐患和装备损耗,提高装备作业安全性及效率。     

猪巴氏杆菌、大肠杆菌二联苗的制备及免疫效果观察

以分离某猪场的多杀性巴氏杆菌、致病性大肠杆菌制备猪巴氏杆菌和大肠杆菌二联灭活苗,同时选择病变明显的病料制备组织灭活苗,探讨它们的免疫效果。结果发现它们的免疫保护率分别为：二联灭活苗为80%以上,组织灭活苗为60%。因此二联苗可试用于控制该猪场巴氏杆菌病的流行。     

DNA amplification fingerprinting and marker screening for pseudo-testcross mapping of flowering dogwood ( Cornus florida L.)

DNA amplification fingerprinting (DAF) with arbitrary oligonucleotide primers was used to study genetic relationships between cultivars of flowering dogwood (Cornus florida L.), evaluate extent of plant hybridization, and generate markers in pseudo-testcross mapping at the intraspecific level. Modified Taguchi optimization methods defined a robust DAF system based on high annealing temperature (48–52 °C) and primer concentration (typically 8 μM) that was used to study genetic diversity of representative dogwood cultivars and hybrids. Phenetic analysis using cluster and numerical methods showed that: (1) cultivars were relatively conserved at the genetic level; (2) their hybridization could be identified in the F1 progeny in the absence of phenotypic or physiological markers; (3) several cultivars grouped according to their recorded ancestry; and (4) dogwood anthracnose-resistant lines originally selected in Catoctin Mountain Park (Maryland) grouped separately from those of southern origin. The DAF protocol was also tested in pseudo-testcross mapping of dogwood at the intraspecific level. A preliminary screening of parents ‘Pink Sachet’ and ‘Fragrant Cloud’ and 7 F1 segregants with 22 octamer primers produced 703 amplified loci, 30 and 39 of which were male and female markers segregating at 1:1 ratios with 98.6% confidence levels in pseudo-testcross configuration. Overall results show that DAF generated markers very efficiently (3 per primer) despite the close relatedness of parental dogwood cultivars. This study constitutes the basis for a future genetic linkage mapping and marker-assisted selection (MAS) effort initially targeted to control important fungal diseases in dogwood. This revised version was published online in July 2006 with corrections to the Cover Date.     

棉花AFLP银染技术及品种指纹图谱应用初报

本文介绍了一种适合于棉花研究的ＡＦＬＰ银染方法,并利用该方法获得了几个棉花品种的指纹图谱     

Molecular characterization and genetic relatedness among walnut (Juglans regia L.) genotypes based on RAPD markers

The potential use of the Randomly Amplified Polymorphic DNA (RAPD) technique for characterization and assessment of genetic relationships was investigated in nineteen walnut (Juglans regia L.) genotypes used as parents or released as cultivars from the breeding program of the University of California at Davis. Most of the 72 decamer primers used yielded scorable amplification patterns based on discernable bands. The results obtained produced a unique fingerprint for each of the walnut genotypes studied. Cluster analysis separated the 19 walnut genotypes into two main groups whose differences were related to their pedigree. Genotypes sharing common parents tend to group together and with at least one of the parents. Thus, RAPD markers can detect enough polymorphism to differentiate among walnut genotypes, even among closely related genotypes, and the genetic similarity based on RAPDs appears to reflect the known pedigree information. RAPD technology can be useful in current walnut breeding programs, allowing the identification of new cultivars as well as the assessment of the genetic similarity among genotypes which will help in selecting the best parents to obtain new genetic combinations. This revised version was published online in July 2006 with corrections to the Cover Date.     

Low level of polymorphism detected by SSR probes in bread wheat

In-gel hybridization patterns were studied in a set of nine diverse bread wheat ( Triticum aestivum L. em. Thell) genotypes using 23 simple sequence repeat (SSR) probes in combination with 14 different restriction enzymes. Multilocus fingerprints due to SSR probes, shown earlier to be characteristic of a majority of plant genomes, were not obtained and only a very low level of polymorphism was detected when using as many as 142 probe-enzyme combinations. The hybridization of a prominent solitary high molecular weight fragment (> 23 kb) with a number of SSR probes suggested the presence of these SSRs (microsatellites) within the long stretches of repeated DNA sequences. This indicates that the genome of bread wheat differs from that of other plants in the organization and distribution of SSRs and that SSR probes detect very little polymorphism.     

草鱼胆汁酸与细菌生长试验

本文研究了草鱼结合胆汁酸直游离胆汁酸盐的抑菌作用,研究结果表明,草鱼结合胆汁酸直力游离胆汁酸酸盐对三种革兰氏阳性菌,如金黄色葡萄球菌、藤黄八叠球菌和苦草孢杆菌有抑菌作用,能够明显地抑制革兰氏阳性菌的生长。草鱼结合胆汁酸盐像乳糖培养基中的牛胆盐一样,能够抑制革兰阳性菌的生长,而有利于革兰氏阴性菌和如大肠杆菌的生长。草鱼游离胆汁酸盐像ＧＮ增菌液中的去氧胆酸钠一样,能够抑制革兰氏阳性菌的生长,而对革兰氏