Biopsy of Morulae and Blastocysts*

Embryo biopsy techniques with special reference to bovine morulae and blastocysts are reviewed. Techniques include methods involving holder pipettes and simplified methods more suitable for field use. The use of embryo biopsy for preimplantation diagnosis in combination with cattle embryo transfer is discussed.     

Detection ofClostridiue chauvoein in formalin-fixed,paraffin-embedded tissues of sheep by the peroxidase-antiperoxidase (PAP) technique

A peroxidase-antiperoxidase (PAP) technique was used to detectClostridium chauvoei in tissue sections from sheep inoculated intramuscularly with a pure culture of this microorganism. Samples of various tissues were taken for bacteriology, histopathology and immunohistochemistry. A primary antiserum againstC. chauvoei for use in the PAP technique was produced in rabbits. Formalin-fixed, paraffin-embedded sections of muscle samples were positively and specifically stained by the PAP technique. The results were consistent with those obtained by bacteriology, but the PAP test was simpler, quicker and less expensive than the bacteriological procedures.The use of the PAP technique would be appropriate for detecting clostridial infections without the constraints of conventional identification methods, especially where laboratory conditions for anaerobic procedures are not readily available.Abbreviations HE haematoxylin and eosin - NS normal rabbit serum - P-ab primary antibody - PAP peroxidase-antiperoxidase - PBS phosphate-buffered saline     

犬结肠双重造影术

对20只本地成年犬进行了结肠双重造影试验.建立了犬结肠双重造影的实施方法.造影剂的剂量以60%(w/v)硫酸钡浆100～200mL、空气100～150mL为宜.利用X线摄片记录了结肠双重造影像,并描述和分析了影像效果.     

犬瘟热的诊疗初探

对16例犬瘟热病犬经临床观察、犬瘟热ELISA试剂盒诊断、包涵体检查和白细胞分类计数等方法进行比较诊断研究。临床表现为肺炎型、肠炎型、神经型和眼型；从上皮组织细胞、嗜中性白细胞、淋巴细胞胞浆和胞核内检查到包涵体；白细胞分类计数结果表明嗜中性白细胞数减少，淋巴细胞和单核细胞数增多；犬瘟热ELISA试剂盒诊断阳性率较低。经治疗试验，探索出治疗各型犬瘟热的系统疗法。     

汝城毛竹枯梢症状诊断与病原鉴定

从湖南汝城病竹上分离的病原菌能完成柯赫氏定律，经ＰＤＡ培养基培养后有性世代的子囊壳及子囊孢子和无性世代的分生孢子盘及分生孢子的形态、大小，与文献中报道的毛竹枯梢病原菌一致．汝城毛竹枯梢是由竹喙球菌（Ｃｅｒａｔｏｓｐｈａｅｒｉａｐｈｙｌｌｏｓｔａｃｈｙｄｉｓｓｐ．ｎｏｖ．）侵染所致．     

兔瘟快速诊断的试验研究

使用醛化人“O”型红细胞和白瓷板进行血凝(HA)和血凝抑制(HI)试验诊断兔瘟,1～2分钟即可判定结果,反应时间比常规法缩短60倍。具有快速定性、定量检测的特点。同时该法特异、快速、简易、经济,是一种易于在基层推广应用的新方法。     

鸵鸟嗜肺性巴氏杆菌病的诊治

嗜肺性巴氏杆菌在兽医病原学中的地位并不受重视 ,对动物的致病作用也很少见报道 ,在鸵鸟病中更是罕见。文章针对一起鸵鸟慢性死亡的病例 ,经临床症状、病理剖检和实验室检验 ,诊断为嗜肺性巴氏杆菌所致 ,并提出了相应的有效防治方案     

犬心丝虫病的诊断与防治

在犬的饲养过程中 ,发现了犬的心丝虫病。再通过对周边地区养犬的调查、剖检 ,掌握了犬心丝虫病的临床症状、实验室诊断方法 ,采取了有效的治疗和预防措施     

A comparison of some of the pharmacokinetic parameters of three commercial sulphadiazine/trimethoprim combined preparations given orally to pigs

Three sulphadiazine/trimethoprim preparations were administered orally during feeding to pigs. Six male and six female pigs were used. Clinically important pharmacokinetic parameters of the two drugs in the three preparations were determined and compared.The plasma concentrations of sulphadiazine and trimethoprim increased rapidly in the pigs followed by a quite rapid decrease from 4 to 12 h after oral administration. The mean values of the absorption half-lives of sulphadiazine and trimethoprim were 0.9–1.6 h and 0.5–0.8 h, respectively. The corresponding values for the elimination half-lives of sulphadiazine and trimethoprim were 3.1–4.3 h and 3.4–6.0 h, respectively. There were no significant differences between the pharmacokinetic parameters of the two compounds in the three preparations with the exception of T_max for sulphadiazine and t_1/2 for trimethoprim. Comparative bioavailability calculations showed no statistically significant differences between sulphadiazine and trimethoprim in the three preparations.The weight increase of the pigs during the experimental period (mean = 37.3–64.9 kg) did not cause differences in the kinetics of the two drugs which could have consequences for the use of the three combined preparations in clinical practice.No unacceptable or antibacterial residues of sulphadiazine or trimethoprim were found in the kidneys of pigs slaughtered at 5, 7 and 10 days after administration.