伪狂犬病病毒引起仔猪腹泻的实验室诊断与分析

对一起伪狂犬病病毒引起仔猪腹泻的病例进行了实验室诊断与分析.结果表明,PRV检测样品中出现270 bp条带,PEDV、TGEV、CSFV、PRRSV和PCV-2检测样品中均未出现目的大小DNA片段,结果均为阴性;细菌性病原检测结果均未见有细菌生长;母猪群gE抗体检测,其中有33份血清检测为PRV gE抗体阳性,阳性率达91.7％.     

一种组合式单(双)铧犁的研究与设计

土壤的翻耕是农业生产中较为重要的环节之一,铧式犁是土壤耕作的主要农机具。分析当前农机市场上单铧犁和双铧犁存在的不足之处,着重论述组合式单(双)铧犁的结构设计,分析该种设计较传统铧犁所拥有的优点。     

基于GPS/BDS的大坝安全监测试验与分析

针对GNSS单系统大坝安全监测易受卫星分布不均致使有效解时段数不足,以及无法准确获取符合大坝mm级监测要求的稳定坐标序列问题,以琅琊山抽水蓄能电站大坝实测数据作为研究对象,研究了基于GPS/BDS组合定位的连续准静态模式在大坝安全监测中的可行性,从可见卫星数量和PDOP值两方面分析了GPS/BDS组合定位较单GNSS系统在大坝安全监测中的优势。对大坝单天安全监测数据进行卡尔曼滤波和坐标均值处理,以及在自主设计的可移动基座架进行滑动试验,结果表明GPS/BDS组合定位在连续准静态模式下通过一段时间的初始测量可以实时获取稳定的大坝监测数据,且具有优于1 mm的监测灵敏度,对分析大坝外部结构长期趋势性变化具有重要意义。     

Laboratory diagnosis of respiratory diseases of cattle

The authors describe the procedure of laboratory diagnosis for bovine respiratory diseases: direct diagnosis by isolation and for identification of bacteria or viruses and indirect diagnosis by serological methods. They specify the restraints and limits of this diagnosis and the significance results which are obtained and connected with knowledge of anamnestic information.     

模糊矩阵在土坝事故诊断中的应用

本文把模糊矩阵法引进土坝事故诊断。在简述模糊矩阵法的基本原理及步骤的基础上,通过土坝事故诊断实例,说明该法对于具有因果关系不太明显、多因多果等复杂情况的工程事故诊断是适用的。在诊断中,考虑了事故征兆并非是简单布尔变量的情况。     

Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology

The polymerase chain reaction has become an important diagnostic tool for the veterinary virologist. Conventional methods for detecting viral diseases can be laborious or ineffective. In many cases PCR can provide a rapid and accurate test. In this article we explain the basic principles of PCR and supply a reference list of its uses in diagnostic veterinary virology.Abbreviations BLV bovine leukaemia virus - BVDV bovine viral diarrhoea virus - DNA deoxyribonucleic acid - ds double-stranded - ELISA enzyme-linked immunosorbent assay - PCR polymerase chain reaction - RNA ribonucleic acid - ss single-stranded - TK thymidine kinase     

空肠弯曲菌共同抗原单克隆抗体的研制及其在细菌检验中应用——Ⅲ.在细菌快速检验中的应用

应用抗空肠弯曲菌共同抗原的单克隆抗体(4A7),建立了适用于空肠弯曲菌快速检验的间接-ELISA和BA-ELISA。两种方法的特异性检查表明,对受试的32种其它细菌,除与幽门弯曲菌发生交叉反应外,其余均为阴性反应。对空弯菌纯培养物检测的敏感性,BA-ELISA为3.2×104个菌/mL,间接-ELISA为1.6×105个菌/mL;对细菌动态培养物检测,BA-ELISA可检出接种约5个菌/mL、间接-ELISA可检出接种约10个菌/mL的24 h增菌培养物。应用BA-ELISA对人(140份)、猪(140份)、禽类(364份)及特种经济动物(265份)标本进行检测,阳性率依次为121%、52%、58.2%和32.8%;应用间接-ELISA对人(140份)、禽类(222份)及特种经济动物(265份)标本进行检测,阳性率依次为11.4%、61.7%、31.3%。两种方法的结果均与常规培养法相差不显著,可在27h内报告结果。     

山羊胚胎性别鉴定取样技术的研究

在进行山羊胚胎性别鉴定研究的同时，对山羊胚胎切割取样技术和切割胚胎的最佳阶段进行了研究，以确定最佳切割时间与取样细胞数。徒手胚胎切割方法取样，经１４０枚切割表明，以６～８日龄胚为好。囊胚和孵出囊胚切割取样成功率最高（９０％），经２２枚取样后的胚胎移植受体，妊娠成功率达３６．４％。     

MDF管道与搅拌联合施胶新工艺试验

在中密度纤维板生产过程中采用管道与搅拌联合施胶新工艺，可以有效地解决产品表面存在的胶斑问题。试验表明，管道施胶部分比例达到４５％－５５％可以取得预期的效果，产品力学性能还有所提高，而胶耗量比传统的管道施胶有明显的减少。     

Diagnostic problems posed by respiratory infections of dogs

The following viruses as well as bacteria and mycoplasma have been isolated from dogs with contagious respiratory disease: canine distemper virus; Canine adenoviruses (type 1 and 2); Parainfluenza type 2 (SV5); Reovirus type 1; Canine Herpesvirus; Bordetella bronchiseptica, Streptococcus, Pasteurella, Staphylococcus and Mycoplasma. The occurrence of these agents can be in direct relationship with: the evolution of a systemic disease; respiratory disorders being a regular or inconsistant symptom of this disease; the evolution of a disease restricted to the respiratory tract; the tropism of the bacterial or viral agent is exclusively respiratory; secondary bacterial complications to a primary viral infection; saprophyte state or latency without pathologic significance. These various infectious agents are implicated alone or in mixed infections and the wide variety of clinical symptoms don't allow to precise a clinical diagnosis. We will try to bring some bases allowing, by the help of laboratory an etiologic diagnosis. This diagnosis is essential for providing an efficient prevention. We will approach some parameters which we have been confronted with as regards Canine Distemper and Canine Adenovirosis. Our purpose is, through these examples of the canine pathology, to confirm and complete some other similar situations which can appear in other animal species.