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901.
适于蛋白质组研究的大豆种子蛋白双向电泳技术的改进 总被引:8,自引:4,他引:8
以大豆种子为材料,通过对样品制备、电泳条件以及染色方法等关键步骤进行改进,在大豆种子蛋白分析研究中获得了质量较高的双向电泳图谱,为大豆种子蛋白质组研究提供一较好的实验方法. 相似文献
902.
J.A. Lucas B. Ramos Solano F. Montes J. Ojeda M. Megias F.J. Gutierrez Mañero 《Field Crops Research》2009
Biocontrol capacity of two plant growth-promoting rhizobacteria (PGPR) strains, against blast disease in rice paddy fields in Southern Spain was studied in three cropping seasons. Both strains (Pseudomonas fluorescens Aur 6 and Chryseobacterium balustinum Aur 9) had already shown biocontrol capacity against pathogens, ability to induce systemic resistance against leaf pathogens and against salt stress in different plant species. Bacterial treatments were carried out on seeds and/or on leaves. Strains were inoculated individually and in combination. Protection against natural disease incidence was evaluated, and rice production and quality measured in 2005 and 2006 trials. In 2004, natural disease incidence was low (between 0.1% and 0.35% of damaged leaf surface) due to environmental conditions; under these conditions, both strains significantly protected plants against rice blast. In 2005, disease incidence was higher than in 2004, reaching higher values of affected leaf surface in controls. In these conditions, each strain individually protected rice against rice blast, although the combination of both strains was the most effective treatment. All three treatments (Aur 6, Aur 9 and Aur 6 + Aur 9) reached 50% protection in panicles, with Aur 9 being the most effective. In 2006, the most effective treatment was the combination of both strains on leaves in three physiological stages, suggesting a biocontrol mediated protection. On the other hand, when bacteria were applied to seeds, disease incidence decreased up to 50%, suggesting induction of systemic resistance. Finally, a direct relation between protection mediated by the PGPR and the increase in rice productivity (mT/ha) and quality (weight of 1000 seeds and number of intact grains after milling) was found. 相似文献
903.
904.
基因枪转化的bar基因表达盒在水稻中的重组结构 总被引:2,自引:1,他引:2
应用Southern杂交和引物组合PCR技术研究了基因枪转化的bar基因表达盒(包括启动子、编码区和终止子)在水稻(Oryza sativa L.)中的重组结构。bar基因表达盒的多个拷贝通过重组连接形成1~3个转基因串联子,彼此邻近整合在受体基因组内一个较大的染色体区域,不同转基因串联子之间由水稻基因组DNA间隔,形成转基因簇。bar基因表达盒形成转基因串联子时,存在头接头、头接尾、尾接尾3种连接方式,通常伴随着bar基因片段的截短。 相似文献
905.
Nimesha Fernando Joe Panozzo Michael Tausz Robert M. Norton Glenn J. Fitzgerald Samuel Myers Cassandra Walker James Stangoulis Saman Seneweera 《Journal of Cereal Science》2012
We investigated wheat (Triticum aestivum) grain quality under Free Air CO2 Enrichment (FACE) of 550 ± 10% CO2 μmol mol−1. In each of two full growing seasons (2008 and 2009), two times of sowing were compared, with late sowing designed to mimic high temperature during grain filling. Grain samples were subjected to a range of physical, nutritional and rheological quality assessments. Elevated CO2 increased thousand grain weight (8%) and grain diameter (5%). Flour protein concentration was reduced by 11% at e[CO2], with the highest reduction being observed at the late time of sowing in 2009, (15%). Most of the grain mineral concentrations decreased under e[CO2] - Ca (11%), Mg (7%), P (11%) and S (7%), Fe (10%), Zn (17%), Na (19%), while total uptake of these nutrients per unit ground area increased. Rheological properties of the flour were altered by e[CO2] and bread volume reduced by 7%. Phytate concentration in grains tended to decrease (17%) at e[CO2] while grain fructan concentration remained unchanged. The data suggest that rising atmospheric [CO2] will reduce the nutritional and rheological quality of wheat grain, but at high temperature, e[CO2] effects may be moderated. Reduced phytate concentrations at e[CO2] may improve bioavailability of Fe and Zn in wheat grain. 相似文献
906.
907.
宁夏小麦品种慢锈基因Lr34/Yr18的分子检测 总被引:1,自引:0,他引:1
Lr34/Yr18是重要的小麦慢叶锈/慢条锈基因,可用于小麦锈病抗性改良。为了明确Lr34/Yr18基因在宁夏小麦中的分布特点,利用STS标记csLV34对111份宁夏小麦品种中慢锈基因Lr34/Yr18的等位变异进行了分子检测,并且进行了成株期条锈病抗性鉴定。结果表明,20份材料携带Lr34/Yr18基因,占18.0%。不同来源的小麦品种中Lr34/Yr18基因分布频率不同,农家品种中分布频率最高,占90.9%;引进品种中所占比例为14.3%;育成品种中所占比例最低,仅占7.7%。含有Lr34/Yr18基因的品种对条锈病具有较好的抗性,可作为今后宁夏小麦抗锈病育种的重要抗源。 相似文献
908.
茶多糖的分级纯化及组成分析 总被引:4,自引:2,他引:4
从粗老绿茶中提取茶叶粗多糖,经DEAE-纤维素DE-52和Sephacryl S-300柱层析纯化,获得三个纯化的茶多糖组分:TPS2-H2O、TPS2-0.25和TPS2-0.40;此三个组分的中性糖含量分别是:41.23%、29.33%和21.39%,糖醛酸含量分别是:19.5%、22.5%和56.4%,蛋白质含量分别是:未检出、0.8%和未检出;GC-MS分析了三个分级组分均由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖和半乳糖等六种单糖构成的杂多糖,其摩尔比分别是TPS2-H2O(21.3:13.8:1.8:23.3:6.9:32.9),TPS2-0.25(60.7:13.4:1.6:2.0:1.2:21.1),TPS2-0.40(60.2:10.8:4.4:3.2:5.7:15.7);HPGPC-ELSD法分析了各组分的分布及所占比重。 相似文献
909.
为了给小麦品质改良提供依据,以强筋小麦品种藁城8901和弱筋小麦品种山农1391为材料,设置不同氮素水平,研究了籽粒谷蛋白大聚合体(GMP)颗粒粒径分布特征及对氮素水平的响应.结果表明,小麦籽粒GMP颗粒粒径范围为0.375~200 μm.GMP颗粒体积分布总体上呈双峰曲线变化,<12 μm的GMP颗粒体积百分比为11.2%~45%,>12 μm的GMP颗粒体积百分比为55.0%~88.8%.与弱筋小麦山农1391相比,强筋小麦藁城8901有较高比例的>12 μm颗粒.GMP颗粒数目分布表现为单峰分布,<4 μm和<12 μm的颗粒数目百分比分别为97.5%~98.0%和99.9%,表明小麦GMP颗粒中绝大多数为<4 μm的小颗粒.在施氮0~240 kg·hm-2范围内,随着施氮量的增加,<12 μm颗粒体积百分比显著降低,12~90 μm、>90 μm颗粒体积百分比增加,说明适量施氮能够显著提高GMP大、中颗粒比例.过量氮素不利于小麦籽粒谷蛋白大聚合体的形成. 相似文献
910.
Phytosulfokine(PSK)is a new peptide plant hormone,which was isolated in the conditioned medium of cultures derived from both monoco-tyledonous and dicotyledonous plants,such as Asparagus officinalis mesophyll[1],rice[2],zinnia[3],and carrot[4].PSK has two types of structure:PSK-αand PSK-β.The former is a sulfated pentapeptide[H-Tyr(SO3H)-Ile-Tyr(SO3H)-Thr-Gln-OH],the latter is a sulfated terapeptide[H-Tyr(SO3H)-Ile-Tyr(SO3H)-Thr-OH].Both are heat-stable,susceptible to pronase… 相似文献