草莓镶脉病毒ORF I基因的克隆、原核表达及抗血清制备

The total DNA was extracted from strawberry leaves infected with Strawberry vein banding virus (SVBV) by CTAB method. Specific primer pairs were designed to amplify the gene ORF I of SVBV-Shenyang isolate by PCR, gene ORF I was cloned into modified prokaryotic expression vector pET-32a (+)-GST, the recombinant plasmid pET-ORF I was transformed into E. coli DH5α, then the positive clones were screened and sequenced. The recombinant plasmid was extracted and transformed into Escherichia coli BL2l (DE3), the fusion protein with an approximate molecular weight of 56 kDa was obtained with IPTG induction and Ni²⁺-NTA affinity column purification. The purified fusion protein was used to immunize the rabbits to prepare the specific antiserum. The result of ELISA showed that the titer of the prepared antiserum is up to 1:520 000, Western blot analysis indicated that the prepared antiserum could reacted specifically with purified recombinant fusion protein. Not only the expression of P1 protein in SVBV infected-strawberry leaves, but also the expression of P1 protein in P1-infiltrated Nicotiana benthamiana leaves could be detected, using 2 000 times diluted antiserum.     

橡胶树优良品种热研7-20-59的选育

热研７－２０－５９是一个速生高产而相对晚熟的橡胶树优良无性系,在基本试区１～９割年干胶产量逐年递增,第９割年平均年产干胶为５．７９ｋｇ／株,２０５７ｋｇ／ｈｍ＾２,分别比对照ＰＲ１０７增产８６．８％和７３．４％;头９割年平均年产干胶３．９５ｋｇ株,１４６７ｋｇ／ｈｍ＾２,分别为对照的１７８．７％和１６８．６％。此外,该品种抗能力较强,风害后有较强的恢复生长能力。在海南农垦１９９８年橡胶树优良品种汇     

Lfy cDNA高效单子叶植物表达载体的构建及转化兰花研究初报

构建一个适合在单子叶植物中高效表达的含有Lfy cDNA的表达载体（pBIL-1),应用基因枪转化法将其导入兰花原球茎中,获量了一些卡那霉素抗性克隆。     

番茄LE-ACC_2合酶基因5′端前导序列分离方法的研究

通过ＰＣ／Ｇｅｎｅ软件对 Ｒｏｔｔｍａｎｎ等人发表的番茄 ＬＥ－ＡＣＣ２合酶的 ５’端 ２．０ Ｋｂ的前导序列作分析,设计了４对特异引物;以番茄果实、叶片的总ＤＮＡ为模板,采用特异ＰＣＲ扩增技术获得了 ２．０, １．８７, １．５８, １．２８ Ｋｂ ４个特异片段,用 Ｔ－Ｖｅｃｔｏｒ技术构建了１个克隆（２．０ Ｋｂ）、３个亚克隆（１．８７,１．５８,１．２８ Ｋｂ）,对４个克隆产物进行了 ＤＮＡ序列测定,借助 ＰＣ／Ｇｅｎｅ软件对所获得的各克隆序列进行综合处理与分析,获得了番茄 ＬＥ－ＡＣＣ２ ５’端前导序列的同源率为９９．９％,但第－９７９位的 Ｃ和第－１０７６位的 Ｔ分别为 Ｔ和 Ｃ所替代。对利用 ＰＣＲ技术分离大片段ＤＮＡ的各环节作了较详细的研究。     

早生优质抗寒茶树新品种"浙农117"选育研究

以福云自然杂交后代为育种材料,经系统选种、无性繁殖育早生优质抗寒茶树良种“浙农１１７”。品比试验与试种结果表明：该品种发芽期特早,适制扁形名茶和大宗红绿茶,品质超过福鼎大白茶;小区产量显著高于对照;茶多酚、咖啡碱、儿茶素平均含量比福鼎大白茶分别高３．３２、０．４５和５５．８９个百分点,氨基酸低０．３６个百分点;抗寒性强,尤其在倒春寒中表现甚佳,抗病虫能力也较强。     

油松雌球果发育对枝条生长的影响

通过对油松种子园无性系生殖生长和营养生长协调关系的研究,发现多数无性系２年生球果的发育对２次芽数、２次顶芽长及侧枝数和当年雌球花芽的分化无显著影响;但２年生球果发育降低当年顶枝生长量,增加侧潜芽数,不同无性系当年生顶枝长,侧枝总长和２次顶芽长差异显著,而２次芽数、侧枝数和侧潜芽数则差异不明显。母枝总干质量和枝长类型对当年生总干质量的直接通径系数分别为１．２３４２和０．４４００,对多元决定系数的直接     

毛白杨优良无性系选择研究

以鲁毛５０为对照,经过８年的观测试验,选出憨白杨、３８号、Ｔ１７３个毛白杨优良无性系。３个无性系的育苗造林成活率达到８５％～１００％,单株材积生长量超过对照７３％～１６５％,均达到差异显著或极显著水平,材积连年生长量从第４年开始迅速增加,形质指标相当于或超过了对照。     

杜梨绿枝扦插快速繁殖的研究

采用当年生杜梨新梢为插条 ,用不同激素和浓度处理后分别插于不同基质中培养。试验结果表明 ,IBA和 NAA对促进杜梨生根均有一定效果 ,以 40 0～ 80 0mg/L IBA效果最好 ,成活率达 83.3%。利用绿枝扦插技术可以快速繁殖生长势一致的杜梨无性系砧木苗