Surgical repair of skull fractures in four horses using cuttable bone plates.

Three horses with severely comminuted, open facial bone fractures and one horse with a comminuted, open orbital rim fracture were referred for treatment. Severe facial bone asymmetry and epistaxis were apparent in all cases and subcutaneous emphysema was present in two, however physical and neurological examinations were otherwise normal. Radiography and endoscopy were of some use in assessing the degree of damage, although the true extent of the damage was more apparent at surgery. Surgical reduction of the fractures was recommended to maximise cosmetic and functional outcome. After surgical debridement and reduction of the fractures the bone fragments remained unstable and were not amenable to stabilisation with interfragmentary wires alone, so 2 mm cuttable bone plates were used to maintain fracture alignment. Screw migration occurred in three horses and in one of these horses the plate had to be removed. Other complications were minor and in all horses the fractures healed with good cosmetic and functional outcome. The use of cuttable bone plates should be considered as a reasonable alternative to inter-fragmentary wiring for unstable, comminuted fractures of the facial bones, even where fractures are open.     

饲粮粗蛋白质、能量水平对3岁梅花公鹿鹿茸生长和体增重的影响

为探讨 3岁梅花鹿生茸期饲粮适宜营养水平 ,本研究采用 2 (CP∶2 1%和 19% )× 2 (GE∶16 74MJ/kg和 15 90MJ/kg)二因子交叉设计 ,选用 3岁 (二锯 )梅花公鹿 6 7头 ,分为 4个试验组 ,进行了饲养试验和消化试验。试验结果表明 ,饲粮蛋白质水平对鹿体增重有显著影响 (P <0 0 5 ) ,在本试验所设能量浓度范围内 ,饲粮蛋白质水平为 19%处理组鹿体增重显著高于 2 1%蛋白组 ;鹿茸产量组间差异不显著 (P >0 0 5 ) ;饲粮粗蛋白质水平对蛋白质消化率和能量消化率均有显著影响 (P <0 0 5 ) ;3岁梅花鹿生茸期饲粮中能量、蛋白质适宜水平分别为 15 9～ 16 7MJ/kg (GE)和 19% (CP) ;平均每头鹿每天对消化能和可消化蛋白质的需要量分别为 2 9 9～ 31 3MJ和 388～ 394 g。     

水貂阿留申病病毒CIEP抗原结合蛋白初探

以水貂阿留申病病毒对流免疫电泳(CIEP)细胞抗原为材料,经酶印迹(Westemblotting)测定,水貂阿留申病病毒CIEI细胞抗原与多克隆阳性血清反应,分子量为60000,50000和25000,而与CIEP阴性的抗水貂阿留申病病毒的单克隆抗体(Y—2—9)反应,分子量为60000,50000.因此初步确定水貂阿留申病病毒CIEP细胞抗原决定族位于分子25000蛋白上.     

Effect of Nitrogen Rate and Stubble Height on Dry Matter Yield,Crude Protein Content and Crude Protein Yield of a Sorghum–Sudangrass Hybrid[Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf.] in the Three‐Cutting System

In this study, the effects of nitrogen (N) rate (60, 120, 180 and 240 kg N ha^?1 applied in three equal dressings at seeding and after the first and second cuttings) and stubble height (7, 14 and 21 cm) on the dry matter (DM) yield, crude protein (CP) content, and CP yield of a sorghum–sudangrass hybrid [Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf., cv. Pioneer 988] in the three‐cut system was investigated. The N rate had no significant effect in the first and third cuttings, but in the second cutting DM yields increased significantly with increase in N rate. The highest yield of 9.1 t ha^?1 was obtained with 80 kg N ha^?1 for the average of 2 years at the second cutting, but no significant difference was found among the 40, 60 and 80 kg N ha^?1 rates. CP content and yield were not significantly affected by N rate at the first and third cuttings, but CP content and yield were significantly affected by application of N at the second cutting. Stubble height had a significant effect on CP content at the third cutting. However, it had no significant effect on CP content at the first and second cuttings. Stubble height had a significant effect on the CP yield at the first cutting, but no significant effect on CP yield at the second and third cuttings.     

猪肺炎支原体膜蛋白的电泳分析及免疫印迹检测

以猪肺炎支原体Z株为试验材料,采用SDS-PAGE和Western印迹对该菌膜蛋白进行了分析。研究表明:猪肺炎支原体经A_(26)液体培养基培养,15000 r/min离心收集菌体细胞,低渗超声法破膜获得膜制剂后,应用12.5％的凝胶对膜蛋白进行SDS-PAGE分离,在电泳图谱上呈现出36条蛋白带,相对分子质量范围为1.18×10~4～9.12×10~4。同时以膜制剂作为抗原,分4次免疫试验兔,心脏采血,提取抗血清,并分离纯化得到IgG,然后进行Western印迹法检测。其结果发现在SDS-PAGE分离得到的36种膜蛋白(或多肽)中,有6种蛋白具有免疫原性。     

生长抑素(SS)与硫氧还原蛋白(Thioredoxin)相融合生成的2种基因工程化融合蛋白SS-N/X和SS-N/S的表达特性

对基因工程化生长抑素（somatostatin,SS)-硫氧还原蛋白（Thioredoxin)融合蛋白SS－N／X和SS－N／S的表达产量、水溶性、Triton X-100对SS融合蛋白水溶性的影响、包涵体的溶解和复性特点以及SS的抗原性与免疫原性等特性进行了研究。结果显示，在30℃的低温下用IPTG诱导表达，SS－N／X融合蛋白主要以可溶性形式存在，占75．8％，色涵体仅占24．2％；而SS－N／S融合蛋白则主要以包涵体的形式存在，占81．1％，可溶性蛋白仅占18．9％；32C载体（pEG-32C)蛋白则居二者之间，可溶性蛋白与包涵体大约等比例出现。SS－N／S在低温下诱导可超量表达SS融合蛋白，其表达量约占菌体总蛋白的40．94％。0．5％ Triton X-100对融合蛋白的不溶性有非常显著的影响，几乎可使所有的SS－N／X融合蛋白和大部分SS－N／S融合蛋白及32C硫氧还原蛋白变成水溶性的。32C硫氧还原蛋白包涵体在尿素中的溶解度明显高于SS－N／S包涵体，2mol/L尿素可使近一半的32C硫氧还原蛋白包涵体溶解，而SS－N／S包涵体只有27．5％溶解；但在5mol/L尿素时，两者均可基本全部变为可溶性的。在SS融合蛋白包涵体溶解时加入还原剂二硫苏糖醇，可明显降低SS的抗原性。SS融合蛋白（水溶性融合蛋白和复性后的包涵体）具有良好的SS抗原性和免疫原性。由SS融合蛋白与弗氏不完全佐剂制成试验用疫苗，免疫BALB／c小鼠、仔猪和羔羊等动物，可显著提高免疫动物的增重。     

家蚕病原性微孢子虫孢子表面蛋白的选择性分离与总蛋白比较分析

选择性分离了Ｎｏｓｅｍａｂｏｍｂｙｃｉｓ孢子表面蛋白和总蛋白，采用ＳＤＳ－ＰＡＧＥ检测发现，孢子表面蛋白由分子量不同的３种亚基（３２０００、２６０００、２４５００Ｄａ）组成，其总蛋白至少包括２５个条带。同时将从养蚕生产中收集到的两种微粒子ＭＡ－１和ＭＤ－１以及从野外昆虫收集到的微孢子虫Ｐｈａ－Ｍ和Ｈａ－Ｍ与Ｎ．ｂｏｍｂｙｃｉｓ进行了比较，发现不同微孢子虫间蛋白组成有差异。     

CLINICAL USE OF 99mTC-MDP SCINTIGRAPHY IN THE EQUINE MANDIBLE AND MAXILLA

^99mTc-methylene diphosphonate (MDP) radionuclide imaging examinations were done in four horses having clinical evidence of skull trauma or infection. Radiographs were made of each horse prior to scintigraphy. Four case histories are presented. In each instance, scintigraphy provided complementary information to that obtainable through radiography, which aided in accurately localizing and characterizing the site and extent of abnormalities, and which proved particularly valuable as an aid for therapeutic planning.     

三种幼虫外寄生蜂蛋白酶含量与离体培养饲料的关系

在离体培养幼虫外寄生蜂过程中，发现不同的蜂对饲料中的蛋白质及游离氨基酸的含量有不同的要求，本文对麦蛾茧蜂（Ｂ．ｈｅｂｅｔｏｒ），矛茧蜂（Ｏ．Ｐｌａｌｉａｔｕｓ），管氏肿腿蜂（Ｓ．ｇｕａｎｉ）的蛋白酶活性，饲料中蛋白质及游离氨基酸含量进行了测定．实验表明：蛋白酶含量低的蜂，饲料中含高游离氨基酸及低蛋白；蛋白酶含量高的蜂，饲料中含低游离氨基酸及高蛋白。     

脱毒桐籽饼(粕)蛋白质及能量营养价值评价

选用8头30kg的阉公猪和6只1.7kg的伊莎褐公鸡分别对3种脱毒桐籽饼(粕)Ⅰ、Ⅱ和Ⅲ的蛋白质、氨基酸消化率、代谢率进行了测定。结果表明 :3种脱毒桐饼(粕)Ⅰ、Ⅱ和Ⅲ的蛋白质含量分别为31.17 %、35.12 %和23.98 % ;总氨基酸含量为226.93mg/g、278.38mg/g和178.71mg/g,其中赖氨酸含量4.14mg/g、5.01mg/g和3.47mg/g ,蛋氨酸含量为1.24mg/g、1.33mg/g和0.92mg/g ;能量(GE)为17.72kJ/g、16.42kJ/g 和18.02kJ/g。脱毒桐粕Ⅰ的蛋白质消化率为75.15 % ,消化能为12.85MJ/kg;脱毒桐饼(粕)Ⅰ、Ⅱ的表观代谢能分别为4.88MJ/kg 和6.29MJ/kg,真代谢能为5.53MJ/kg 与6.94MJ/kg。3种脱毒桐饼(粕)的氨基酸之间存在不平衡状况 ,从限制性氨基酸的角度来看 ,脱毒桐饼(粕)的第一限制性氨基酸是蛋氨酸、第二限制性氨基酸为赖氨酸。