参与碳氮磷转化的水解酶对不同施肥响应的差异

本文旨在研究土壤水解酶对不同施肥的响应差异以及影响因素。通过在红壤中添加牛粪有机肥、化肥进行90d的室内土壤培养试验,采用微孔板荧光法动态分析5、30和90d参与碳氮磷转化的土壤水解酶(α-1,4-葡萄糖苷酶、β-1,4-葡萄糖苷酶、纤维素酶、木聚糖酶、亮氨酸氨基肽酶、β-1,4-N-乙酰氨基葡萄糖苷酶、磷酸酶)活性。与不施肥(对照)相比,在30 d后,化肥处理的总酶活性显著下降,对应的参与碳氮磷转化酶活性均有不同程度下降;而有机肥处理的总酶活性在培养期内均未发生显著变化,但是其α-1,4-葡萄糖苷酶显著增加,而磷酸酶活性显著降低。参与碳转化的4种水解酶中,只有α-1,4-葡萄糖苷酶活性对施肥的响应较强,且施加有机肥增加其活性而无机肥则降低其活性;对于参与氮转化的水解酶而言,化肥明显抑制了亮氨酸氨基肽酶活性,而有机肥增加了β-1,4-N-乙酰氨基葡萄糖苷酶活性;磷酸酶活性明显受到有机肥的抑制作用,而对化肥的响应总体不明显。不同水解酶对不同施肥的响应有明显差异,NMDS分析表明,α-1,4-葡萄糖苷酶和亮氨酸氨基肽酶响应最明显,其次为磷酸酶与木聚糖酶;相关和冗余分析显示,土壤p H、可溶性有机碳对酶活性的影响最大,一定程度说明了不同肥料通过影响土壤理化性质进而影响水解酶活性。     

Modern wheat cultivars have greater root nitrogen uptake efficiency than old cultivars

The availability of nitrogen (N) contained in crop residues for a following crop may vary with cultivar, depending on root traits and the interaction between roots and soil. We used a pot experiment to investigate the effects of six spring wheat (Triticum aestivum L.) cultivars (three old varieties introduced before mid last century and three modern varieties) and N fertilization on the ability of wheat to acquire N from maize (Zea mays L.) straw added to soil. Wheat was grown in a soil where ¹⁵N‐labeled maize straw had been incorporated with or without N fertilization. Higher grain yield in three modern and one old cultivar was ascribed to preferred allocation of photosynthate to aboveground plant parts and from vegetative organs to grains. Root biomass, root length density and root surface area were all smaller in modern than in old cultivars at both anthesis and maturity. Root mean diameter was generally similar between modern and old cultivars at anthesis but was greater in modern than in old cultivars at maturity. There were cultivar differences in N uptake from incorporated maize straw and the other N sources (soil and fertilizer). However, these differences were not related to variation in the measured root parameters among the six cultivars. At anthesis, total N uptake efficiencies by roots (total N uptake per root weight or root length) were greater in modern than in old cultivars within each fertilization level. At maturity, averaged over fertilization levels, the total N uptake efficiencies by roots were 292?336 mg N g^?1 roots or 3.2?4.0 mg N m^?1 roots for three modern cultivars, in contrast to 132?213 mg N g^?1 roots or 0.93?1.6 mg N m^?1 roots for three old cultivars. Fertilization enhanced the utilization of N from maize straw by all cultivars, but root N uptake efficiencies were less affected. We concluded that modern spring wheat cultivars had higher root N uptake efficiency than old cultivars.     

Application of urease inhibitor improves protein composition and bread‐baking quality of urea fertilized winter wheat

Background: Nitrogen losses is an economic problem for wheat production and a high risk to the environment. Therefore, improved N fertilizer management is a key to increasing the N efficiency and minimizing N losses. To increase N efficiency, enhanced fertilizers such as urea combined with urease inhibitor can be used. Aims: The aim of present study was to evaluate the effects of different N forms on grain storage protein subunits in winter wheat and to examine whether the observed changes correlate with parameters of baking quality. Methods: The investigation was performed over two consecutive years at two locations in Germany. Protein subunits were analyzed by SDS‐PAGE. Results: Protein concentrations were similarly increased after fertilization with ammonium nitrate and urea + urease inhibitor. Analysis of the individual storage protein fractions indicated that both fertilizers specifically enhanced ω‐gliadins and HMW glutenins, but the effect was more pronounced in the ammonium nitrate treatment. Application of urea + urease inhibitor had greater influence on the protein composition and resulted in higher specific baking volume as well as the best fresh keeping ability, in comparison with urea treatment. Conclusion: Considering that the urea + urease inhibitor treatment resulted in almost comparable improvements of NUE and baking quality, with the additional benefit of reduced N losses in combination with easy handling, urea + urease inhibitor can be recommended as a viable alternative to both urea alone and ammonium nitrate treatments. This opens up an opportunity for the reduction of N loss in wheat production when use of urea is preferred.     

京津风沙源区生态保护与建设工程对防风固沙服务功能的影响

[目的] 评价京津风沙源生态保护与建设工程自2000年启动实施近20 a以来的防风固沙效应,以指导工程二期的实施。[方法] 选取植被覆盖度、风蚀量和防风固沙服务功能保有率等指标进行分析。[结果] 京津风沙源区以草地为主,其次为林地和农田;工程实施以来,多年平均土壤风蚀量为7.87×10⁸ t,以微度和轻度侵蚀为主;一期工程实施期间的土壤风蚀量总体呈逐年减小趋势,二期工程实施以来,风沙源区遭受风蚀危害又逐渐加重,尤其是沙化草原亚区,该区风蚀模数变化趋势达到了8.96 t/（hm²·a）;就防风固沙服务功能保有率而言,整个风沙源区均值达到了0.82,低值区主要分布于沙化草原亚区（0.743）和晋北山地丘陵亚区（0.752）;二期工程实施以来,大部分区域保有率均显著提升,这与二期工程实施期间全年及冬春季的植被覆盖度变化情况一致。[结论] 京津风沙源的风蚀防治区重点在保有率下降区域和以草地和沙地为主的沙化草原亚区、浑善达克沙地亚区和科尔沁沙地亚区。     

菌剂挂膜3D-RBC联合BCO工艺处理养猪沼液废水

针对养猪沼液废水寡营养、高氨氮的水质特征,该研究采用耐高氨氮、适应贫营养生长的异养硝化-好氧反硝化（Heterotrophic Nitrification-Aerobic Denitrification,以下简称HN-AD）菌挂膜启动三维结构生物转盘+生物接触氧化反应器（3D-RBC+BCO）组合工艺对沼液进行处理。该文研究了3D-RBC+BCO组合工艺在真实沼液条件下的启动过程及污染物去除效果,重点考察了溶解氧（Dissolved Oxygen,DO）浓度和C/N比2个关键因素对组合工艺污染物去除效果的影响。同时,借助高通量测序技术对DO和C/N比优化过程中微生物群落结构的变化规律进行解析。结果表明：在真实沼液条件下,采用HN-AD菌剂挂膜启动方法,仅用12和18 d就分别完成3D-RBC和BCO反应器的挂膜启动,同时组合工艺对COD、NH4+-N和TN的去除率分别稳定在94.8%、95.7%和80.1%,出水优于城镇污水厂排放一级B标准。在对3D-RBC反应器DO和C/N比的优化过程中,增设底曝后COD、NH4+-N和TN等指标的去除率分别降低了25.4%、15.4%和15.5%。高通量测序结果显示,增加底曝后3D-RBC盘片生物膜中微生物菌属的数量小幅下降,但HN-AD优势菌属的种类与丰度显著降低,导致脱氮效率下降;贫营养型Acinetobacter、Pseudomonas菌属是3D-RBC可以对真实沼液高效脱氮的关键,提高C/N比会显著降低其丰度,进而影响脱氮效果。     

盐碱地玉米产量及土壤硝态氮对深松耕作和秸秆还田的响应

【目的】盐碱地是我国重要的土地资源,研究合理可行的耕作技术和培肥措施,为提高产量和实现盐碱地农业可持续发展提供理论依据。【方法】2014-2017年,在山东省滨州市无棣县的盐碱土上,以玉米为供试作物,连续进行了4年田间试验。试验采用裂区设计,以耕作方式为主区,分别设夏季旋耕15 cm (R)和旋耕后再深松35 cm处理(S);副区为冬季秸秆还田量,设秸秆半量还田(3350 kg/hm^2,B)和秸秆全量还田(6700kg/hm^2,Q)两个用量,以无秸秆还田为对照。所有处理的养分总量保持一致。开花后每隔10天取样1次,直到收获期,测定植株干物质和穗位叶硝酸还原酶活性。在玉米小口期、开花期及收获期,取0-100 cm土层样品,每10 cm为一层,测定了土壤硝态氮含量及累积量。【结果】各生育期玉米干物质积累量和产量在两个耕作方式间的差异不显著,与秸秆半量还田相比,秸秆全量还田处理开花期前干物质量较少,但在开花期时,已经开始赶超秸秆半量还田的处理。在收获期,秸秆全量还田处理的干物质量显著高于秸秆半量还田处理的干物质量,4年中的提高幅度为8.6%~9.7%,秸秆全量还田处理的籽粒产量显著优于秸秆半量还田处理(P <0.05)。4年干物质积累量,SQ处理平均比SB、RQ和RB分别提高2.5%~7.3%、1.6%~4.2%和7.6%~20.3%。深松与秸秆全量还田有明显的正耦合作用,秸秆全量还田与深松耕作相结合有利于籽粒产量的提高,与其他处理差异显著(P <0.05)。相同耕作方式下,4年中秸秆全量还田处理的平均硝态氮含量在小口期低于秸秆半量还田,在开花期显著高于秸秆半量还田,但在收获期又显著低于秸秆半量还田的处理,硝态氮累积量平均降低17.9%(P <0.05)。在4年中深松耕作处理的0-100 cm平均硝态氮累积量比旋耕处理的显著降低8.9%。【结论】在供试盐碱地土壤条件下,秸秆全量还田结合浅旋耕后再深松的效果最好,可提高玉米产量,减少土壤中硝态氮的累积。     

长江中下游平原单季稻田氮素径流损失风险评估

稻田氮素径流损失是农业面源污染主要来源之一,以巢湖地区单季稻田为研究对象,利用该地区1957—2019年的历史气象数据,通过设定插秧区间(6月6—25日)及施肥期水位(3,10,20 cm),建立SMNRL模型,模拟不同插秧时间和田面水水位稻田氮素流失,研究降低长江中下游平原气候区单季稻田氮素径流损失风险的插秧时间与水位控制模式。结果表明:(1)施肥后,稻田田面水氮素浓度呈指数衰减,基肥期田面水氮素衰减期为9天,分蘖肥和穗肥期为7天。(2)在LW、HW组合中,各施肥期占全生育的氮素径流损失为基肥期>分蘖肥期>穗肥期。在LW组合中,基肥期为氮素径流损失高发期,基肥、分蘖肥、穗肥的氮素流失为72.4%~98.4%,1.9%~27.6%,0~8.3%。(3)控制水位比选择插秧时间对降低氮素径流损失更有效。相同水位下,适宜的插秧期氮素径流损失在全生育期施肥中合计能减少0.4~4.5 kg/hm^2,降低32.8%~80.3%;相同插秧时间下,LW、MW组合相比HW组合氮素径流损失能减少8.8~13.1 kg/hm^2,降低92.1%~98.8%。(4)在LW、MW、HW 3种组合中,插秧期分别以6月19日、6月11日、6月17日为界,将6月6—25日分为前后2个阶段,前1阶段插秧产生氮素径流损失均值显著低于后1阶段,分别低37.0%,25.0%,21.7%。(5)降低巢湖地区稻田氮素径流损失有效措施为施肥期水位控制为3 cm,并选择6月6-19日期间进行水稻插秧。     

鲁西平原毛白杨造林地土壤全氮空间变异性研究

以位于鲁西平原冠县毛白杨6.0hm2造林地为研究区,将土壤在水平方向按50m×50m分为24块样地,每块样地分为0～20cm,20～40cm,40～60cm三层取样,进行三维空间采样和室内土壤全氮含量测定,对土壤全氮空间分异规律进行研究。结果表明:研究区土壤全氮在林地尺度存在一定的空间变异;从研究区土壤总体分异状况看,土壤全氮含量范围是0.02～0.46gkg-1,总体均值为0.17gkg-1,三个层次土壤全氮含量分别为0.33±0.06gkg-1,0.14±0.04gkg-1,0.05±0.02gkg-1,全氮含量属于最低水平。三个层次土壤全氮含量变异系数分别为19%、32%、39%,变异程度属于中等变异水平,土壤全氮随着土层加深分异程度明显增大。土壤全氮含量在不同土壤层次水平变化复杂,0～20cm层西北高,东南部低,20～40cm和40～60cm两层变化恰好与之相反。研究结果可为毛白杨造林试验地布设、养分精准管理、确定合理采样数提供依据。     

三江平原典型碟形湿地土壤氮素分布特征

以三江平原典型碟形沼泽湿地为研究对象,分析了湿地土壤氮素的分布特征和影响因素,及其与湿地演替的关系。结果表明:土壤TN和NH4+-N含量在碟形湿地内的分布规律是一致的,均表现为随着湿地水位的降低而逐渐降低,随着土壤深度的增加而降低。受水位波动的影响沼泽化小叶章湿地土壤NO3--N含量降低明显,而永久淹水湿地表层土壤该形态氮含量较下层高。碟形湿地土壤各形态氮素的分布受到土壤有机质含量、水分特征、生物量分布特征等多种因素的影响。     

Involvement of Bradyrhizobium japonicum denitrification in symbiotic nitrogen fixation by soybean plants subjected to flooding

Denitrification by Bradyrhizobium japonicum bacteroids contributes to nitric oxide (NO) production within soybean nodules in response to flooding conditions. However, the physiological relevance of NO production by denitrification in B. japonicum-Glycine max symbiosis is still unclear. In this work, soybean plants were inoculated with B. japonicum strains lacking the nirK or norC genes which encode the copper-containing nitrite reductase and the c-type nitric oxide reductase enzymes, respectively. 14 days flooding increased nodule number of plants inoculated with the WT and norC strains, but not of plants inoculated with the nirK mutant. However, nodule dry weight was not affected by 14 days flooding regardless of the strain used for inoculation. Supporting this observation, individual nodule growth was significantly higher in plants inoculated with nirK than those inoculated with WT or norC after 14 days flooding. Nodule functioning was strongly inhibited by flooding since leghemoglobin content of the nodules induced by any of the strains was significantly decreased after 7 or 14 days flooding compared to control plants. However, this effect was more relevant in nodules of plants inoculated with the WT or norC mutant than in those inoculated with the nirK mutant. Nitrogen fixation was also estimated by analyzing nitrogen content derived from biological nitrogen fixation in shoots, using the ¹⁵N isotope dilution technique. By using this approach, we observed that the negative effect of 14 days flooding on nitrogen fixation was more pronounced in plants inoculated with the norC mutant. However, nitrogen fixation of plants inoculated with nirK showed the highest tolerance to 14 days flooding. These findings allowed us to demonstrate the previously proposed hypothesis which suggests that NO formed by copper-containing nitrite reductase in soybean nodules, in response to flooding, has a negative effect on nitrogenase activity. We propose that inoculation of soybeans with a B. japonicum nirK mutant, which does not produce NO from nitrate, increases the tolerance of symbiotic nitrogen fixation to flooding.