蛋氨酸羟基类似物的生产工艺及其在动物营养中的应用

蛋氨酸羟基类似物(MHA)作为蛋氨酸(Met)替代物被广泛应用于饲料添加剂中.MHA因其特殊的结构性质,不仅具备Met的营养功能,而且可以发挥Met不具备的酸化剂、抗生素等功能.随着我国畜牧和饲料添加剂行业的发展,MHA合成方法的研究及应用必将得到进一步的关注,因此,本文概述了MHA的生产工艺及其在动物营养中的应用.     

Effects of Tannic Acid on Active Aluminum Species Distribution in Various Tea Soils

Effects of tannic acid on active aluminum species distribution in different pH tea soils in Yunnan and Jiangxi tea gardens were studied by field observation and laboratory analysis.There were 4 species of active aluminum in the tea soil,i.e.,exchangeable aluminum Al3＋,unimer hydroxyl aluminum Al（OH）2＋ Al（OH）2＋,acid-soluble aluminum Al（OH）30 and humic-acid aluminum Al-HA.Experimental results showed that tannic acid at levels 0 ～0.4 mmol/kg and 0 ～ 2.0 mmol/kg,with the rising of soil pH value,the content of exchangeable aluminum decreased significantly,hydroxyl aluminum,acid-soluble aluminum and humic-acid aluminum were increased gently.When the concentration of tannic acid increased to above 2.0 mmol/kg,the inhibition of higher concentration of tannic acid on content of active aluminum was strengthened with the rising of soil pH value and variation law of 20 ～40 cm soil layer was similar to 0 ～20cm soil layer.Generally speaking,the content of active aluminum in upper layer of soil were more than subsoil and total content of active aluminum in Yunnan tea soils were higher than Jiangxi tea soils.The correlation analysis showed that the soil pH were positively correlated with hydroxyl aluminum,humic-acid aluminum,pHBC （r =0.796,0.960,0.852 ; p ＜ 0.01,0.01,0.01） in 0 ～ 20 cm soil layer.pHBC were significant negative correlated with exchangeable aluminum （r =-904,p ＜ 0.01）,hydroxyl aluminum （r =-645,p ＜ 0.05）,and with humic-acid aluminum have a positive correlation,correlation coefficient was0.795 （P ＜ 0.05）.Meanwhile,the tannic acid addition concentration were 0 ～ 0.4 mmol/kg soils pH were increased significantly,while samples attains a peak pH value afterwards decrease continuously,the relationship between the pH and the concentration of tannic acids accords with the equation：YpH =-0.04CDN ＋3.82（R2 =0.95,P ＜0.01）.When the concentration of tannic acid up to about 8.0 ～ 12.0 mmol/kg,soil pH keep unchanged.Therefore,we can clearly draw that effects of tannic acid on active aluminum content of the inflection point is not the same in different region of tea garden soil.Low concentration tannic acid can improve the soil content of various forms of aluminum,but with the increase in the amount of tannic acid,the various forms of aluminum content are inhibited.With the soil pH increased,high concentrations tannic acid on the inhibition of the release of active aluminum increased.pH and tannic acid on the total amount of active aluminum garden soil was mutual weakening effect relationship.     

Physiological mechanisms for antagonistic interaction of manganese and aluminum in barley

In a previous study, we found that the combined addition of Al and Mn in the culture solution could alleviate the inhibition of barley growth by addition of Al or Mn alone. The current experiment was conducted in a greenhouse to investigate the physiological mechanisms of the antagonistic interaction using two barley genotypes, XZ16 (both Al and Mn tolerant) and ZU9 (both Al and Mn sensitive). The treatments consisted of three Al levels (0, 0.1, and 0.5?mM) and three Mn levels (0, 0.2, and 1.0?mM) and their combinations, and a completely randomized block design was used with three replications. The combined treatments had larger plant biomass, lower Al and Mn concentrations and accumulation in plant tissues, lower malondialdehyde content, and higher root ATPases activities, compared with Al or Mn alone treatment. The two genotypes had the similar trend in the antagonistic interaction, with ZU9 being more predominant than XZ16.     

林木对铝的耐性机理研究进展

在酸性土壤中,铝对林木的毒害是限制林木生长、降低林木生产力、进而导致森林退化的主要因素之一。目前,国内外在林木铝毒害及耐铝机制方面已有较多的研究,但关于林木耐铝的生理和分子机制的综述性报道很少。文中综述了近年来林木对铝的富集、铝对林木生理和分子水平上的毒害等方面的研究进展,总结了林木耐铝的生理机制和分子机制,简要介绍了外源添加物对林木耐铝毒的调控机制,提出了今后有关林木铝毒需进一步研究的重点,以期为林木铝毒的深入研究提供参考以及为缓解我国酸性土壤林木铝毒害及森林的健康持续经营提供参考。     

Extraction of mobile element fractions in forest soils using ammonium nitrate and ammonium chloride

The extraction of earth alkaline and alkali metals (Ca, Mg, K, Na), heavy metals (Mn, Fe, Cu, Zn, Cd, Pb) and Al by 1 M NH₄NO₃ and 0.5 M NH₄Cl was compared for soil samples (texture: silt loam, clay loam) with a wide range of pH(CaCl₂) and organic carbon (OC) from a forest area in W Germany. For each of these elements, close and highly significant correlations could be observed between the results from both methods in organic and mineral soil horizons. The contents of the base cations were almost convertible one‐to‐one. However, for all heavy metals NH₄Cl extracted clearly larger amounts, which was mainly due to their tendency to form soluble chloro complexes with chloride ions from the NH₄Cl solution. This tendency is very distinct in the case of Cd, Pb, and Fe, but also influences the results of Mn and Zn. In the case of Cd and Mn, and to a lower degree also in the case of Pb, Fe, and Zn, the effect of the chloro complexes shows a significant pH dependency. Especially for Cd, but also for Pb, Fe, Mn, Zn, the agreement between both methods increased, when pH(CaCl₂) values and/or contents of OC were taken into account. In comparison to NH₄Cl, NH₄NO₃ proved to be chemically less reactive and, thus, more suitable for the extraction of comparable fractions of mobile heavy metals. Since both methods lead to similar and closely correlated results with regard to base cations and Al, the use of NH₄NO₃ is also recommended for the extraction of mobile/exchangeable alkali, earth alkaline, and Al ions in soils and for the estimation of their contribution to the effective cation‐exchange capacity (CEC). Consequently, we suggest to determine the mobile/exchangeable fraction of all elements using the NH₄NO₃ method. However, the applicability of the NH₄NO₃ method to other soils still needs to be investigated.     

黑麦品种间耐铝差异性机制研究

采用室内模拟方法,研究了铝诱导黑麦根系分泌有机酸、根尖磷对铝的固定作用。结果表明,在铝胁迫下冬牧品种（Win）相对根伸长率高于King品种。在铝胁迫下,经磷预处理的根尖铝和磷含量增加,且以Win品种根尖中的Al、P含量较高,说明根尖磷对铝的固定是黑麦耐铝机制之一。另一方面,在铝胁迫下,两品种根系均分泌柠檬酸和苹果酸,且Win的分泌速率较高。有机酸的分泌随着铝处理浓度（10、30、50 mol/L）和时间（0.5、3、6、9、12 h）的增加而增加,但在低温（4C）下柠檬酸分泌量显著减少。Al处理0.5 h后苹果酸已明显分泌,而柠檬酸的分泌在铝处理6 h后才明显增加。在铝处理前,进行缺磷预处理（3 d）不能增加有机酸的分泌,10 mol/L的La3+、Cu2+、Ni3+也不能诱导根系分泌有机酸。说明铝诱导根系专一性分泌有机酸也是黑麦品种间耐铝性差异的机制。     

Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken

1. The effect of supplementing water-soluble vitamin E analogues 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox) and butylated hydroxy toluene (BHT) was studied in two separate experiments.

2. In the first experiment, trolox was supplemented at 0.2 mM, 0.4 mM and 0.8 mM concentrations along with N-methylacetamide (MA; 12% final concentration) and semen was cryopreserved in 0.5 ml French straws. Different semen parameters and fertility were assessed from post-thaw samples.

3. Sperm motility, live sperm, and mitochondrial activity were significantly lower (P < 0.05) in cryopreserved semen. Lipid peroxidation (LPO) was significantly higher (P < 0.05) in cryopreserved semen that was reduced by trolox supplementation. The treatment containing trolox at 0.2 mM concentration produced significantly higher (P < 0.05) fertility compared to unsupplemented cryopreservation treatment.

4. In the second experiment, BHT was supplemented at 0.25 mM, 0.5 mM, and 1 mM concentrations along with MA during semen cryopreservation.

5. Sperm motility, live sperm and MTT dye reduction test were significantly lower (P < 0.05) in cryopreserved semen. These parameters declined with increasing BHT concentration. Abnormal sperm was significantly higher (P < 0.05) in the BHT supplemented treatments. The sperm chromatin dispersion (SCD) test was significantly higher (P < 0.05) in cryopreserved samples and was highest in samples supplemented with 0.5 mM and 1 mM BHT. The percentage fertility was significantly lower (P < 0.05) in cryopreserved semen and BHT supplementation did not improve fertility.

6. In conclusion, trolox supplementation at 0.2 mM concentration during semen cryopreservation improved fertility, whereas BHT supplementation resulted in a decline in post-thaw semen parameters.     

施用碱稳定污泥污水土壤经γ-辐照后土壤溶液中Cu和Zn

Soil samples collected from several acid soils in Guangdong, Fujian, Zhejiang and Anhui provinces of the southern China were employed to characterize the chemical species of aluminumions in the soils. The proportion of monomeric inorganic Al to total Al in soil solution was in the range of 19% to 70%, that of monomeric organic Al (Al-OM) to total Al ranged from 7.7% to 69%, and that of the acid-soluble Al to total Al was generally smaller and was lower than 20% in most of the acid soils studied. The Al-OM concentration in soil solution was positively correlated with the content of dissolved organic carbon (DOC) and also affected by the concentration of Al³⁺. The complexes of aluminum with fluoride (Al-F) were the predominant forms of inorganic Al, and the proportion of Al-F complexes to total inorganic Al increased with pH. Under strongly acid condition, Al³⁺ was also a major form of inorganic Al, and the proportion of Al³⁺ to total inorganic Al decreased with increasing pH. The proportions of Al-OH and Al-SO₄ complexes to total inorganic Al were small and were not larger than 10% in the most acid soils. The concentration of inorganic Al in solution depended largely on pH and the concentration of total F in soil solution. The concentrat ions of Al-OM, Al³⁺, Al-F and Al-OH complexes in topsoil were higher than those in subsoil and decreased with the increase in soil depth. The chemical species of aluminumions were influenced by pH. The concentrations of Al-OM, Al³⁺, Al-F complexes and Al-OH complexes decreased with the increase in pH.     

Effects of dietary methionine hydroxy analogue supplement on growth,protein deposition and intestinal enzymes activities of juvenile Jian carp (Cyprinus carpio var. Jian)

A total of 900 juvenile Jian carp (Cyprinus carpio var. Jian) (8.24 ± 0.03 g) were fed practical diets containing graded levels of methionine hydroxy analogue (MHA) (0, 5.1, 7.6, 10.2, 12.7 and 15.3 g kg^?1 diet) for 60 days to investigate the effects of MHA on growth, protein deposition and intestinal enzymes activities. Per cent weight gain (PWG) significantly increased with increasing levels of MHA up to a point (P < 0.05), and thereafter declined. Feed efficiency was the highest when MHA level was at 5.1 g kg^?1 diet. Feed intake (FI) significantly enhanced with dietary MHA level up to a point (P < 0.05), beyond which it plateaued. Patterns of differences in protein production value, lipid production value, intestosomatic index, folds height, lipase, chymotrypsin, γ‐glutamyl transpeptidase, Na⁺, K⁺‐ATPase, creatinekinase, glutamate‐oxaloacetate transaminase and glutamate‐pyruvate transaminase activities were the same as that in PWG, whereas the trend of plasma ammonia content was opposite. Hepatopancreas protein content, trypsin and amylase activities followed the same trend as that of FI. The optimal supplemental level of MHA for fish meal and miscellaneous meals–based diet (6.9 g methionine kg^?1 diet) for PWG was 8.2 g kg^?1 diet by the quadratic regression analysis.     

坛紫菜中铝的形态分析及风险性评价

应用化学连续萃取法结合电感耦合等离子体质谱(ICP-MS)法对紫菜中铝的存在形态进行了初步研究,并对紫菜中铝食用安全性进行了评价。所测30份紫菜样品中铝的总含量范围为74.3~1 479 mg/kg,紫菜中铝的形态存在难溶态>有机态>无机态>水溶性游离态的分布规律。约75%的铝以难溶态存在。可溶态的铝主要以有机铝的形态存在,有机铝占总可溶态铝的72%左右。无机铝主要以Al(OH)₃的形态存在,水溶性游离态铝占无机铝的18.5%。通过模拟胃肠消化环境测定紫菜中铝的溶出率,结果表明,模拟胃液可溶出的铝占总铝的1.38%～6.80%,说明大部分铝在人体消化过程中不易溶出,对人体的潜在危害并不大。